aAlthough some genes were chosen for their possible relevance to tumor phenotype, genes were primarily intended to serve as markers for regions of DNA amplification or deletion. ^bChromosomal locations of genes are indicated by arrows in Fig. 3a in the accompanying publication. ^cDNA copy number determination by quantitative PCR (S. Gelmini et al., 1997, Clin Chem 43, 752-8) was performed using a TaqMan instrument (ABI), with PE Applied Biosystems TaqMan PCR reagents and protocols. Gene-specific primers and fluorogenic probes were designed from 3’UTR DNA sequences using PrimerExpress (ABI) software. Relative gene copy numbers were derived using the formula 2^DCT where DCT is the difference in amplification cycles required to detect amplification product from equal starting concentrations of normal genomic DNA compared to tumor genomic DNA (R.M. Pitti et al., 1998, Nature 396, 699-703). All assays were performed in duplicate; mean values are reported.