Header Line Info

• EXP Name of the experiment, based on the array id.
• NAME Name of the sample that is at a given position.
• TYPE Indicates whether a element is a control.
• CH1I The average total signal for each spot for Cy3
• CH1B The local background calculated for each spot for Cy3
• CH1D The difference between the total and background for each element.
• CH2I The average total signal for each spot for Cy5.
• CH2B The local background calculated for each spot for Cy5
• CH2D The difference between the total and background for each element.
• CH2IN The average total signal for each spot for Cy5 normalized so that the average log ratio of all well measured spots is equal to 0.
• CH2BN The local background calculated for each spot for Cy5 normalized so that the average log ratio of all well measured spots is equal to 0.
• CH2DN The difference between the total and background for each element normalized so that the average log ratio of all well measured spots is equal to 0.
• RAT1 CH1D/CH2D
• RAT2 CH2D/CH1D
• RAT1N CH1D/CH2DN
• RAT2N CH2DN/CH1D
• CRT1 and CRT2 Unused.
• CORR The correlation of the signal at each pixel of a spot in CH1 to CH2.
• REGR The ratio estimated by the slope of the line fit to the distribution of pixels in each spot.
• RFLAG Unused.
• PLAT The plate ID from which a sample was printed.
• PROW The plate row from which a sample was printed.
• PCOL The plate column from which a sample was printed.
• FLAG A user defined field which identifies spots which are visually of low quality. The value 0 is good, all other values represent poor quality spots.
• SIZE The size (in bp) of the gene.
• SPOT The spot number on the array.
• LEFT The left coordinate of the spot.
• TOP The top coordinate of the spot.
• RIGHT The right coordinate of the spot.
• BOT The bottom coordinate of the spot.
• FAILED Identifies spots for which the PCR is not likely to have worked. A value of 0 is good, a value of 1 indicates no PCR product was observed, and a value of more than 1 indicates how many distint bands were visualed by gel electrophoresis.