| Figure 3 Genomic targets of SBF and MBF. Percentile ranks of intergenic fragments that met selection thresholds are indicated (blue-yellow colour scale). Loci with greater than 70% overall nucleotide sequence identity to another yeast locus (potentially cross-hybridizing), are indicated (closed circles). The combination of Cy3 and Cy5 labeled probes, the antibody used for immunoprecipitation (if used) and the culture conditions for each experiment are summarized (left panel). Experiments 9, 10, 17 and 18 involved independent crosslinking and immunoprecipitations. DNA microarrays that included all yeast ORFs and other features in addition to the intergenic fragments were used for experiments 3, 8, 13 and 14. |
