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Cells were grown in the chemostat with glucose limitation then harvested and fixed in formaldehedye. Cells were stained with DAPI to compare at the abundance of mitochondrial DNA in the parental and evolved strains. Imaging was performed on a deltavision microscope (Applied Imaging Systems) and twenty 0.2 micron sections were deconvolved for DAPI images. Isolates from the parental (P) and three evolved populations E1, E2, and E3 respectively.
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