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Methods
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Tissues
and mRNA isolation:
Patients gave informal consent to participant in these studies before
the surgery. All tissues were surgically resected, snap frozen in liquid
nitrogen within a half hour after the resection, and stored in -80oC.
In most of the cases, both tumor and adjacent non-tumor tissues were collected.
Total RBA was extracted with RNeasy kit (Qiagne) and mRNA was isolated
from total RNA using FastTrack (Invitorgen) or Poly(A)Pure (Ambion) mRNA
purification kit. mRNA from cell lines was purified directly with FastTrack
(Invitrogen) kit. We combined mRNA from the following cells, in equal
quantities, to make the reference pool:
HepG2: HCC cell line
SNU398: HCC cell line
SNU1: Gastric adenocarcinoma cell line
Jurkat: T-cell line
RPMI: B-cell line
CCD-1070SK: Fibroblast cell line
Microarray Procedure
23075 c
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