Figure 1: Stereotyped host responses to diverse heat-killed bacteria. Stereotyped host responses to diverse heat-killed bacteria. PBMCs isolated from an apheresis sample from a single healthy donor were treated with the heat-killed bacteria B. pertussis LPS (BpLPS)(1 ug/ml), B. pertussis strain 338 (Bp338)(0.35 particles/cell), B. pertussis Minnesota1 isolate (BpMinn1)(1.6 particles/cell), E. coli clinical isolate (0.4 particles/cell), S. aureus clinical isolate 1 (1.6 particles/cell), S. aureus clinical isolate 2 (2.5 particles/cell), and ionomycin (1uM) plus PMA (25ng/ml). For the mock time course, PBMCs were treated with sterile liquid media. Time point measurements were collected at 0, 0.5, 1, 2, 4, 6, 12, and 24 hours. High quality data were selected and zero-transformed as described (see Methods) and a gene variation filter was defined based the occurrence of at least 4 measurements varying at least 2.5-fold from the mean across all stimulus time points. a, The expression of the resulting 920 cDNAs is displayed in hierarchical cluster format. Each row represents a single array element and each column a separate experimental mRNA sample. Experiments are organized by increasing time within each time course, the first column representing the zero time point, the last representing the 24-hour time point. Each expression measurement represents the ratio of fluorescence from the hybridized experimental sample to the reference sample, and is displayed as relative to the averaged zero time point. As indicated, the scale extends from fluorescence ratios of -2 to +2 in log2 units. Gray indicates missing or excluded data. b, and c, represent the common induction and common repression clusters, respectively, which are expanded portions of the larger cluster. Genes of interest are indicated by their names.