Figure2: Human mast cells stimulated via the FceRI secrete IL-11.
(A) Production of IL-11 by 3 different populations (from Donors 4- 6) of human umbilical cord blood-derived mast cells (> 99% purity) that were incubated for 6 h without anti-IgE (“0”) or were maintained for 6 h after stimulation via the FceRI with anti-IgE at 10 mg/ml. Data represent mean + s.e.m. for 2-3 determinations for each population. (B) Kinetics of IL-11 production in human umbilical cord blood-derived mast cells after stimulation via the FceRI with anti-IgE at 1 mg/ml. Data represent mean + s.e.m. for 3 different populations of human mast cells (from Donors 7- 9). Values for anti-IgE stimulated vs. control cells were analyzed for statistical significance by the Student’s t test, 2-tailed.