E3 spores frogged to glucose and acetate minimal plates 

Plate schematic (A, G, or D before the E3 spore represents the carbon source onto which the tetrad was dissected: acetate, glycerol, or glucose, respectively.

AE3.4A AE3.4B AE3.4C AE3.4D
GE3.4ADE3.12A DE3.12B DE3.12C DE3.12D CP1AB
GE3.4CGE3.1B GE3.1C GE3.1D CP1AB
E3 (flocculent)E3 (flocculent) E3 (flocculent) E3 (not flocculent) E3 (not flocculent) E3 (not flocculent)
DE3.1ADE3.1C DE3.1D DE3.2B DE3.2C DE3.2D
GE3.3A GE3.3B GE3.3C GE3.3D

0.5% glucose minimal plate at 25 degees



1% acetate plate at 25 degrees



0.5% glucose minimal plate at 37 degrees



1% acetate plate at 37 degrees