Paquin, Adams, and Rosenzweig derived all of the evolved strains in this study by growing strain CP1AB in a glucose-limited chemostat at a dilution rate of 0.2/hour. A concentration of 8 g/L glucose was used. For more information about exactly how the experiments were done, see Paquin and Adams 1982 (CP1AB derivation) and 1983 (E1, E4-E8 derivation) and Ferea et al. 1999 (E2 and E3 derivation).