Figure 1
The same section of the microarray is shown for three independent hybridizations comparing RNA isolated at the 8 hour time-point after serum treatment to RNA from serum-deprived cells. Each microarray contained 9996 elements, including 9804 human cDNAs, representing 8613 different genes. mRNA from serum-deprived cells was used to prepare cDNA labeled with Cy3-dUTP and mRNA harvested from cells at different times after serum stimulation was used to prepare cDNA labeled with Cy5-dUTP. The two cDNA probes were mixed and simultaneously hybridized to the microarray. The image of the subsequent scan shows genes whose mRNAs are more abundant in the serum-deprived fibroblasts (i.e., suppressed by serum treatment) as green spots and genes whose mRNAs are more abundant in the serum treated fibroblasts appear as red spots. Yellow spots represent genes whose expression does not vary significantly between the two samples. The arrows indicate the spots representing the following genes: 1) Protein disulphide isomerase-related protein P5, 2) Interleukin-8 precursor, 3) EST AA057170, 4) Vascular endothelial growth factor.