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ID   CARNEGIE1  preliminary; circular DNA; SYN; 3616 BP.
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AC   M30841; ATCC37238;
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DT   15-MAR-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Drosophila/E.coli plasmid vector Carnegie 1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3616
RC   pCIW1 from pUC8 & p6.1
RC   pCIW2 from pCIW1
RC   pCIW3 from pCIW2
RC   pCIW4 from pCIW3 & linker
RC   pV9, pV10, pV11 from pCIW4
RC   pV1 from pCIW4 & pUC8
RC   Carnegie 1 from pCIW4 & pUC8
RC   Carnegie 2 from Carnegie 1 & linker
RC   Carnegie 3 from Carnegie 1 & linker
RC   Carnegie 4 from Carnegie 1 & M13mp10
RC   Carnegie 20 or car20 from Carnegie 2 & Drosophila rosy gene
RA   Rubin G.M., Spradling A.C.;
RT   "Vectors for P element-mediated gene transfer in Drosophila";
RL   Nucleic Acids Res. 11:6341-6351(1983).
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CC   This is a plasmid vector containing a non-autonomous P element from
CC   Drosophila melanogaster.  It should be used with a helper plasmid such
CC   as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (Carnegie 1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli K-12)(Drosophila melanogaster)(E.coli)
CC   CP ()
CC   FN (cloning)(transposable element)
CC   SE ()
CC   PA (pUC8)(Drosophila P element)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. non-autonomous P element in white locus
FT                   -> p6.1
FT                   1. pUC8 SalI 2665bp 247..247
FT                   2. p6.1 SalI-SalI, P element in white locus
FT                   -> pCIW1 5300bp
FT                   1. pCIW1 BamHI
FT                   BAL31 nuclease
FT                   -> pCIW2 4600bp
FT                   1. pCIW2 SalI
FT                   BAL31 nuclease
FT                   -> pCIW3 3900bp
FT                   1. pCIW3 PvuII
FT                   EcoRI linker 6bp gaattc
FT                   -> pCIW4 3900bp
FT                   1. pCIW4 remove small EcoRI-HindIII
FT                   2. pUC8 EcoRI-HindIII 30bp 231..261, MCS
FT                   -> Carnegie 1 3616bp"
FT   rep_origin      1..856
FT                   /note="ORI Drosophila P element"
FT   misc_recomb     856..857
FT                   /note="Drosophila P element DNA end/Drosophila white
FT                   locus DNA start"
FT   misc_recomb     1358..1359
FT                   /note="Drosophila white locus DNA end/pUC8 DNA start"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             1763..2623
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_recomb     3388..3389
FT                   /note="pUC8 DNA end/Drosophila white locus DNA start"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
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SQ   Sequence 3616 BP; 975 A; 857 C; 833 G; 951 T; 0 other;
     catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
     aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
     aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
     ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
     agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
     attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
     cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
     ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
     ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
     aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcccgggga
     tccgtcgacc tgcagccaag ctttgcgtac tcgcaaatta ttaaaaataa aactttaaaa
     ataatttcgt ctaattaata ttatgagtta attcaaaccc cacggacatg ctaagggtta
     atcaacaatc atatcgctgt ctcactcaga ctcaatacga cactcagaat actattcctt
     tcactcgcac ttattgcaag catacgttaa gtggatgtct cttgccgacg ggaccacctt
     atgttatttc atcatggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg
     gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg
     tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc aaaaagctaa
     tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa gtatttcccc
     ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac ttacacacct
     gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata tattttttat
     atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca ccacgtttcg
     tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt ttgttcgacg
     accttggagc gactgtcgtt agttccgcgc gattcggtgc ggtatttcac accgcatatg
     gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     aggggggcgg agcctatgga aaaacgcctt cttcttgaac tcgggctcgg tgccagtata
     cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa
     ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc
     cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga
     ctacgtgggt ctggcc
//