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ID   CARNEGIE2  preliminary; circular DNA; SYN; 3622 BP.
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AC   M30841; ATCC37239;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Drosophila/E.coli plasmid vector Carnegie 2 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCIW1 from pUC8 & p6.1
RC   pCIW2 from pCIW1
RC   pCIW3 from pCIW2
RC   pCIW4 from pCIW3 & linker
RC   pV9, pV10, pV11 from pCIW4
RC   pV1 from pCIW4 & pUC8
RC   Carnegie 1 from pCIW4 & pUC8
RC   Carnegie 2 from Carnegie 1 & linker
RC   Carnegie 3 from Carnegie 1 & linker
RC   Carnegie 4 from Carnegie 1 & M13mp10
RC   Carnegie 20 from Carnegie 2 & Drosophila rosy gene
RA   Rubin G.M., Spradling A.C.;
RT   "Vectors for P element-mediated gene transfer in Drosophila";
RL   Nucleic Acids Res. 11:6341-6351(1983).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   This is a plasmid vector containing a non-autonomous P element from
CC   Drosophila melanogaster.  It should be used with a helper plasmid such
CC   as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (Carnegie 2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli K-12)(Drosophila melanogaster)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Carnegie 1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Carnegie 1 SmaI 3616bp 596..596, MCS
FT                   HpaI linker 6bp gttaac
FT                   -> Carnegie 2 3622bp"
FT   -               1..595
FT                   /note="Carnegie 1 1..595 595bp
FT                   SmaI = CCC^GGG
FT                   HpaI =     GTT^AAC"
FT   -               596..601
FT                   /note="gttaac 6bp
FT                   HpaI = GTT^AAC
FT                   SmaI =     CCC^GGG"
FT   -               602..3622
FT                   /note="Carnegie 1 596..3616 3021bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-BamHI-HpaI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-BamHI-HpaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 3622 BP; 977 A; 858 C; 834 G; 953 T; 0 other;
     catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
     aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
     aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
     ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
     agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
     attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
     cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
     ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
     ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
     aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcccgttaa
     cggggatccg tcgacctgca gccaagcttt gcgtactcgc aaattattaa aaataaaact
     ttaaaaataa tttcgtctaa ttaatattat gagttaattc aaaccccacg gacatgctaa
     gggttaatca acaatcatat cgctgtctca ctcagactca atacgacact cagaatacta
     ttcctttcac tcgcacttat tgcaagcata cgttaagtgg atgtctcttg ccgacgggac
     caccttatgt tatttcatca tggtctggcc attctcatcg tgagcttccg ggtgctcgca
     tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac
     tgcttgtttt tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa
     agctaatgta attatatttg tgccaataaa aacaagatat gacctataga atacaagtat
     ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac
     acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt
     ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac
     gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt
     tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg
     catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     ctcgtcaggg gggcggagcc tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc
     agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
     gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
     gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc
     gctggactac gtgggtctgg cc
//