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ID   CARNEGIE3  preliminary; circular DNA; SYN; 3624 BP.
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AC   M30841; ATCC37240;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Drosophila/E.coli plasmid vector Carnegie 3 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCIW1 from pUC8 & p6.1
RC   pCIW2 from pCIW1
RC   pCIW3 from pCIW2
RC   pCIW4 from pCIW3 & linker
RC   pV9, pV10, pV11 from pCIW4
RC   pV1 from pCIW4 & pUC8
RC   Carnegie 1 from pCIW4 & pUC8
RC   Carnegie 2 from Carnegie 1 & linker
RC   Carnegie 3 from Carnegie 1 & linker
RC   Carnegie 4 from Carnegie 1 & M13mp10
RC   Carnegie 20 or car20 from Carnegie 2 & Drosophila rosy gene
RA   Rubin G.M., Spradling A.C.;
RT   "Vectors for P element-mediated gene transfer in Drosophila";
RL   Nucleic Acids Res. 11:6341-6351(1983).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   This is a plasmid vector containing a non-autonomous P element from
CC   Drosophila melanogaster.  It should be used with a helper plasmid such
CC   as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (Carnegie 3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli K-12)(Drosophila melanogaster)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Carnegie 1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Carnegie 1 SmaI 3616bp 596..596, MCS
FT                   KpnI linker 8bp cggtaccg
FT                   -> Carnegie 3 3624bp"
FT   -               1..595
FT                   /note="Carnegie 1 1..595 595bp
FT                   SmaI = CCC^GGG
FT                   KpnI =     cGGTAC^Cg"
FT   -               596..603
FT                   /note="cggtaccg 8bp
FT                   KpnI = cGGTAC^Cg
FT                   SmaI =       CCC^GGG"
FT   -               604..3624
FT                   /note="Carnegie 1 596..3616 3021bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-BamHI-KpnI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-BamHI-KpnI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 3624 BP; 976 A; 860 C; 836 G; 952 T; 0 other;
     catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
     aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
     aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
     ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
     agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
     attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
     cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
     ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
     ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
     aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttccccggta
     ccgggggatc cgtcgacctg cagccaagct ttgcgtactc gcaaattatt aaaaataaaa
     ctttaaaaat aatttcgtct aattaatatt atgagttaat tcaaacccca cggacatgct
     aagggttaat caacaatcat atcgctgtct cactcagact caatacgaca ctcagaatac
     tattcctttc actcgcactt attgcaagca tacgttaagt ggatgtctct tgccgacggg
     accaccttat gttatttcat catggtctgg ccattctcat cgtgagcttc cgggtgctcg
     catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
     actgcttgtt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa
     aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt
     atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt
     acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata
     ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc
     acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt
     gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggtgcgg tatttcacac
     cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     tgctcgtcag gggggcggag cctatggaaa aacgccttct tcttgaactc gggctcggtg
     ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg
     ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc
     acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg
     ccgctggact acgtgggtct ggcc
//