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ID   CARNEGIE4  preliminary; circular DNA; SYN; 3631 BP.
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AC   M30841; ATCC37241;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Drosophila/E.coli plasmid vector Carnegie 4 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCIW1 from pUC8 & p6.1
RC   pCIW2 from pCIW1
RC   pCIW3 from pCIW2
RC   pCIW4 from pCIW3 & linker
RC   pV9, pV10, pV11 from pCIW4
RC   pV1 from pCIW4 & pUC8
RC   Carnegie 1 from pCIW4 & pUC8
RC   Carnegie 2 from Carnegie 1 & linker
RC   Carnegie 3 from Carnegie 1 & linker
RC   Carnegie 4 from Carnegie 1 & M13mp10
RC   Carnegie 20 or car20 from Carnegie 2 & Drosophila rosy gene
RA   Rubin G.M., Spradling A.C.;
RT   "Vectors for P element-mediated gene transfer in Drosophila";
RL   Nucleic Acids Res. 11:6341-6351(1983).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   This is a plasmid vector containing a non-autonomous P element from
CC   Drosophila melanogaster.  It should be used with a helper plasmid such
CC   as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (Carnegie 4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli K-12)(Drosophila melanogaster)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Carnegie 1)(M13mp10)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Carnegie 1 remove EcoRI-HindIII 30bp
FT                   \ 589..619, MCS/3586bp
FT                   2. M13mp10 EcoRI-HindIII 45bp 6232..6277, MCS
FT                   -> Carnegie 4 3631bp"
FT   -               1..588
FT                   /note="Carnegie 1 1..588 588bp
FT                   EcoRI = G^AATTC"
FT   -               589..633
FT                   /note="M13mp10 6232..6276 45bp
FT                   HindIII = A^AGCTT"
FT   -               634..3631
FT                   /note="Carnegie 1 619..3616 2998bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-XbaI-BamHI-SmaI-SstI-
FT                   EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-XbaI-BamHI-SmaI-
FT                   SstI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 3631 BP; 978 A; 862 C; 837 G; 954 T; 0 other;
     catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
     aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
     aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
     ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
     agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
     attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
     cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
     ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
     ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
     aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcgagctcg
     cccggggatc ctctagagtc gacctgcagc ccaagctttg cgtactcgca aattattaaa
     aataaaactt taaaaataat ttcgtctaat taatattatg agttaattca aaccccacgg
     acatgctaag ggttaatcaa caatcatatc gctgtctcac tcagactcaa tacgacactc
     agaatactat tcctttcact cgcacttatt gcaagcatac gttaagtgga tgtctcttgc
     cgacgggacc accttatgtt atttcatcat ggtctggcca ttctcatcgt gagcttccgg
     gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc
     cgaaataact gcttgttttt ttttttacca ttattaccat cgtgtttact gtttattgcc
     ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa
     tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa
     aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg
     ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca
     ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc
     accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggtgcggtat
     ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccttcttct tgaactcggg
     ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga
     gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc
     gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc
     cgatctgccg ctggactacg tgggtctggc c
//