back Return to this vector's summary.
Contributed by:

 \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \
William R. Engels                     :     WREngels@facstaff.wisc.edu
Genetics Department, 445 Henry Mall   :         office: (608) 263-2213
University of Wisconsin               :            lab: (608) 262-5578
Madison, WI  53706                    :            FAX: (608) 262-2976
----------------------------------------------------------------------
DNA sequence  pP[CaSpeR]   7742  b.p. complete sequence

Reference: Pirrotta, V., 1988  Vectors for P-mediated transformation in
Drosophila. Biotechnology. 10: 437-456.

This is the entire circular plasmid sequence, starting with the P 3' end. 
The P[CaSpeR] transposon itself is in bp 1 -  4982
The white gene is in 5' to 3' orientation.

Sequencing was done by Greg Gloor, Dena Johnson-Schlitz and Nadine
Nassif in the Engels lab.

LOCUS       pCASPER     7742 BP   DNA                         PRELIMINARY
DEFINITION  pCASPER VECTOR
BASE COUNT    1985 a   1836 c   1780 g   2141 t
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcaggtc gactctagag gatccccggg cgagctcgaa ttctagtatg tatgtaagtt
      301 aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg
      361 gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt
      421 accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt
      481 tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc
      541 tttcgccacc gtttgtagcg ttacctagcg tcaatgtccg ccttcagttg cactttgtca
      601 gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc
      661 caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt
      721 tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg
      781 agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta
      841 gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg caacatcaaa
      901 ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaatcg cacaaacaaa
      961 tagtgacacg aaacagatta ttctggtagc tgtgctcgct atataagaca atttttaaga
     1021 tcatatcatg atcaagacat ctaaaggcat tcattttcga ctacattctt ttttacaaaa
     1081 aatataacaa ccagatattt taagctatgc acaaaaaata aataaaagta taaacctact
     1141 tcgtaggata cttcgttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     1201 agatccccta tcattgcagg gtgacagcgg acgcttcgca gagctgcatt aaccagggct
     1261 tcgggcaggc caaaaactac ggcacgctcc tgccacccag tccgccggag gactccggtt
     1321 cagggagcgg ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg
     1381 gggcggtcaa tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat
     1441 tctgcaacga gcgacacata ccggcgccca ggaaacattt gctcaagaac ggtgagtttc
     1501 tattcgcagt cggctgatct gtgtgaaatc ttaataaagg gtccaattac caatttgaaa
     1561 ctcagtttgc ggcgtggcct atccgggcga acttttggcc gtgatgggca gttccggtgc
     1621 cggaaagacg accctgctga atgcccttgc ctttcgatcg ccgcagggca tccaagtatc
     1681 gccatccggg atgcgactgc tcaatggcca acctgtggac gccaaggaga tgcaggccag
     1741 gtgcgcctat gtccagcagg atgacctctt tatcggctcc ctaacggcca gggaacacct
     1801 gattttccaa gccatggtgc ggatgccacg acatctgacc tatcggcagc gagtggcccg
     1861 cgtggatcag gtgatccagg agctttcgct cagcaaatgt cagcacacga tcatcggtgt
     1921 gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat tcgcctccga
     1981 ggcactaacc gatccgccgc ttctgatctg cgatgagccc acctccggac tggactcatt
     2041 taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca agaccgtcat
     2101 cctgaccatt catcagccgt cttccgagct gtttgagctc tttgacaaga tccttctgat
     2161 ggccgagggc agggtagctt tcttgggcac tcccagcgaa gccgtcgact tcttttccta
     2221 gtgagttcga tgtgtttatt aagggtatct agcattacat tacatctcaa ctcctatcca
     2281 gcgtgggtgc ccagtgtcct accaactaca atccggcgga cttttacgta caggtgttgg
     2341 ccgttgtgcc cggacgggag atcgagtccc gtgatcggat cgccaagata tggcgacaat
     2401 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     2461 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     2521 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     2581 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     2641 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     2701 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     2761 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     2821 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     2881 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     2941 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     3001 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     3061 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     3121 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     3181 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     3241 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     3301 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     3361 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     3421 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     3481 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     3541 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     3601 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     3661 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     3721 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     3781 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     3841 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     3901 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     3961 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     4021 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     4081 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     4141 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     4201 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     4261 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     4321 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     4381 aactagaatt aattcggctg ctgctctaaa cgacgcattt cgtactccaa agtacgaatt
     4441 ttttccctca agctcttatt ttcattaaac aatgaacagg acctaacgca cagtcacgtt
     4501 attgtttaca taaatgattt tttttactat tcaaacttac tctgtttgtg tactcccact
     4561 ggtatagcct tcttttatct tttctggttc aggctctatc actttactag gtacggcatc
     4621 tgcgttgagt cgcctccttt taaatgtctg accttttgca ggtgcagcct tccactgcga
     4681 atcattaaag tgggtatcac aaatttggga gttttcacca aggctgcacc caaggctctg
     4741 ctcccacaat tttctcttaa tagcacactt cggcacgtga attaatttta ctccagtcac
     4801 agctttgcag caaaatttgc aatatttcat ttttttttat tccacgtaag ggttaatgtt
     4861 ttcaaaaaaa aattcgtccg cacacaacct ttcctctcaa caagcaaacg tgcactgaat
     4921 ttaagtgtat acttcggtaa gcttcggctt tcgacgggac caccttatgt tatttcatca
     4981 tgggccagac ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg
     5041 atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc
     5101 tccacgtccg cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg
     5161 tacgacaacc atttgaggta tactggcacc gagcccgagt tcaagaagaa ggcgtttttc
     5221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5641 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5881 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5941 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6001 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6061 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6121 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6181 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6241 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6301 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6361 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6421 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6481 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6541 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6601 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6661 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6721 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6781 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6841 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6901 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6961 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     7021 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     7081 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     7141 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     7201 attgtactga gagtgcacca tatgcggtgt gaaataccgc accgaatcgc gcggaactaa
     7261 cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag
     7321 acagcgaaag agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc
     7381 gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt
     7441 ttttgatgta aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta
     7501 cttgtgggga tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt
     7561 ggcacaaata taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa
     7621 taatggtaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg
     7681 ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag
     7741 ac
//