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ID   COLE1      preliminary; circular DNA; PRO; 6646 BP.
XX
AC   J01566; M33100;
XX
DT   04-AUG-1986 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector Colicin E1 (ColE1) - complete.
XX
KW   cloning vector.
XX
OS   Escherichia coli
OC   Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RP   1008-1370
RC   pNT1 or pVH51 from pML21, ColE1 ori
RA   Tomizawa J.I., Ohmori H., Bird R.E.;
RT   "Origin of replication of colicin E1 plasmid DNA";
RL   Proc. Natl. Acad. Sci. U.S.A. 74:1865-1869(1977).
XX
RN   [2]
RP   1410-1599
RC   ColE1 from E. coli, relaxation site
RA   Bastia D.;
RT   "Determination of restriction sites and the nucleotide sequence
RT   surrounding the relaxation site of ColE1";
RL   J. Mol. Biol. 124:601-639(1978).
XX
RN   [3]
RP   623-832
RC   ColE1 from E. coli P678-54, colicin gene
RC   pAO100 or pVH51 from pML21, mini-ColE1 segment
RC   pAO2 [6] from pAO100
RA   Morita M., Oka A.;
RT   "The structure of a transcriptional unit on colicin E1 plasmid";
RL   Eur. J. Biochem. 97:435-443(1979).
XX
RN   [4]
RP   1-1613, 6577-6646
RC   pAO2, pAO3 from ColE1, ori
RC   pAO7 from pI258
RA   Oka A., Nomura N., Morita M., Sugisaki H., Sugimoto K.,
RA   Takanami M.;
RT   "Nucleotide sequence of small ColE1 derivatives: structure of the
RT   regions essential for autonomous replication and colicin E1
RT   immunity";
RL   Mol. Gen. Genet. 172:151-159(1979).
XX
RN   [5]
RP   1-192, 384-519, 6644-6646
RC   ColE1 from E. coli, initiation/termination
RA   Patient R.K.;
RT   "Characterization of in vitro transcription initiation and
RT   termination sites in Col E1 DNA";
RL   Nucleic Acids Res. 6:2647-2665(1979).
XX
RN   [6]
RP   4783-5353
RC   ColE1 from E. coli, colicin E1 gene promoter
RA   Ebina Y., Kishi F., Miki T., Kagamiyama H., Nakazawa T.,
RA   Nakazawa A.;
RT   "The nucleotide sequence surrounding the promoter region of colicin
RT   E1 gene";
RL   Gene 15:119-126(1981).
XX
RN   [7]
RP   639-753
RC   ColE1 from E. coli, replication
RA   Tomizawa J.I.;
RT   "Control of ColE1 plasmid replication: the process of binding of RNA
RT   I to the primer transcript";
RL   Cell 38:861-870(1984).
XX
RN   [8]
RP   5046-5110
RC   ColE1 from E. coli, colicin E1 gene promoter
RA   Parker R.C.;
RT   "Mitomycin C-induced bidirectional transcription from the colicin E1
RT   promoter region in plasmid ColE1";
RL   Biochim. Biophys. Acta 868:39-44(1986).
XX
RN   [9]
RP   57-123
RC   pXT5 from ColE1 & pBR322
RC   pSZ7 from pXT5
RA   Zhiang S., Yan L., Zubay G.;
RT   "Regulation of gene expression in plasmid ColE1: delayed expression
RT   of the kil gene";
RL   J. Bacteriol. 170:5460-5467(1988).
XX
RN   [10]
RC   ColE1 from E. coli, replication sites
RA   Inoue N., Uchida H.;
RT   "Transcription and initiation of ColE1 DNA replication in
RT   Escherichia coli K-12";
RL   J. Bacteriol. 173:1208-1214(1991).
XX
RN   [11]
RC   ColE1
RA   Lebowitz J.;
RT   ;
RL   Submitted (04-APR-1985) to GenBank on computer-readable media by:
RL   Lebowitz J., .
XX
RN   [12]
RC   ColE1 from E. coli, relaxation sites
RC   ColE2 from E. coli, relaxation sites
RA   Lovett M.A., Guiney D.G., Helinski D.R.;
RT   "Relaxation complexes of plasmids ColE1 and ColE2: unique site of
RT   the nick in the open circular DNA of the relaxed complexes";
RL   Proc. Natl. Acad. Sci. U.S.A. 71:3854-3857(1974).
XX
CC   ColE1 plasmid makes Colicin E1, which inhibits other E. coli
CC   strains. Cells with the plasmid have immunity to Colicin E1.
CC   This is the L (light) strand.
CC   The open reading
CC   frames noted by [8] are named in FEATURES by the molecular mass of
CC   their predicted products or by the names given by [8].
CC   NM (ColE1)(Colicin E1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (L)(light)
CC   TY (plasmid)
CC   SP (Sigma)
CC   HO (E.coli C600)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (colicin E1)
CC   BR ()
CC   OF (ColE1Amp)
CC   OR (2 bp 3' to EcoRI site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, colicin gene
FT                   -> ColE1 6646bp"
FT   mRNA            complement(<59..409)
FT                   /note="RNA E. coli imm mRNA [4],[8]"
FT   CDS             complement(59..400)
FT                   /note="GEN E. coli colicin E1 immunity protein (imm)"
FT   misc_binding    0..0
FT                   /note="SIT AvaI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT SacII"
FT   misc_binding    0..0
FT                   /note="SIT ScaI"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   CDS             448..585
FT                   /note="GEN E. coli kil protein gene"
FT   misc_RNA        641..>641
FT                   /note="RNA E. coli RNAII"
FT   misc_RNA        complement(645..751)
FT                   /note="RNA E. coli RNAI (3' end +/- 1 bp) [5]"
FT   rep_origin      1197..1197
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT                   (+/- 1 bp) [1]"
FT   mRNA            1449..>3936
FT                   /note="RNA E. coli mob mRNA [4] [8];
FT                   5' end putative"
FT   misc_feature    complement(1465..1466)
FT                   /note="SIT E. coli relaxation (nic) cut site [2]"
FT   CDS             1609..1746
FT                   /note="GEN E. coli mob1 protein gene;
FT                   (GTG start codon)"
FT   CDS             complement(1612..1803)
FT                   /note="GEN E. coli RNAI inhibition modulator protein
FT                   gene; (rom - GTG start codon)"
FT   mRNA            complement(<3943..4887)
FT                   /note="RNA E. coli exc mRNA [4],[8]"
FT   CDS             complement(3943..4362)
FT                   /note="GEN E. coli entry exclusion protein 2 (exc2)"
FT   CDS             complement(4443..4862)
FT                   /note="GEN E. coli entry exclusion protein 1 (exc1)"
FT   mRNA            join(5056..6646,1..>585)
FT                   /note="RNA E. coli ColE1-kil mRNA [8]"
FT   misc_binding    5063..5093
FT                   /note="SIT LexA binding site [8]"
FT   mRNA            join(5056..6646,1..>585)
FT                   /note="RNA E. coli ColE1-kil mRNA [8]"
FT   CDS             join(5140..6646,1..62)
FT                   /note="GEN E. coli colicin E1 protein (cea)"
XX
SQ   Sequence 6646 BP; 1760 A; 1421 C; 1798 G; 1667 T; 0 other;
     ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
     aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
     aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
     ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
     ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
     ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
     ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
     ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
     ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
     gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
     acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
     ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
     ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
     ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
     cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
     gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc
     attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa
     tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt
     cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct
     cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
     aagcgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag
     cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
     cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc
     gagaggatgc tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt
     gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt
     tccggaaaac tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat
     aacctgaatc agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt
     caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggctgtc
     agggaacagg gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag
     tggtccggtt gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca
     gggtataccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac
     gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat
     ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg
     ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct
     acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc
     ttggcagacg ctgttaaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg
     caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt
     cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat
     tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat
     tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt
     tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa
     tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg
     agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa
     aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg
     cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg
     ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg
     aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga
     acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga
     aagagtgggt acatctgaag gagtgacaca gagtgacaga gctggaaaca catttgctga
     acgccttaga gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct
     tcgtggaatt gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc
     gggtcatgca gttgagtcag caggtgcaac acttgagcga gcagacagaa cgcttgagcc
     agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgt
     gttatcccta gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggcccttc
     gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
     cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgc
     agtttttagg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
     gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
     gccatcgaga taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag
     gtctttgctg cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa
     ttcttggctg tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc
     tttgtcttta gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt
     gtcggctttt tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg
     ccgggtatgt tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt
     tatcatggcg ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta
     aagtatttat tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc
     gttcagcgac cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc
     ctgagctggg ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc
     atatcctgtg cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagt
     tcccggagaa tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc
     ctttctggtg tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca
     aactcccgtc gtccgtccct gccggtacgg taactgacaa ggcctccgcg catatcacgg
     taaaactgtg aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc
     ataaagtgac agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt
     ctgagaacgg taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt
     gaaagactgt ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa
     atgcagcggc gtagctttta tgctgtatat aaaaccagtg gttatatgta cagtatttat
     ttttaactta ttgttttaaa agtcaaagag gattttataa tggaaaccgc ggtagcgtac
     tataaagatg gtgttcctta tgatgataag ggacaggtaa ttattactct tttgaatggt
     actcctgacg ggagtggctc tggcggcgga ggtggaaaag gaggcagtaa aagtgaaagt
     tctgcagcta ttcatgcaac tgctaaatgg tctactgctc aattaaagaa aacacaggca
     gagcaggctg cccgggcaaa agctgcagcg gaagcacagg cgaaagcaaa ggcaaacagg
     gatgcgctga ctcagcgcct gaaggatatc gtgaatgagg ctcttcgtca caatgcctca
     cgtacgcctt cagcaacaga gcttgctcat gctaataatg cagctatgca ggcggaagca
     gagcgtttgc gccttgcgaa agcagaagaa aaagcccgta aagaagcgga agcagcagaa
     aaggcttttc aggaagcaga acaacgacgt aaagagattg aacgggagaa ggctgaaaca
     gaacgccagt tgaaactggc tgaagctgaa gagaaacgac tggctgcatt gagtgaagaa
     gctaaagctg ttgagatcgc ccaaaaaaaa ctttctgctg cacaatctga agtggtgaaa
     atggatggag agattaagac tctcaattct cgtttaagct ccagtatcca tgcccgtgat
     gcagaaatga aaacgctcgc tggaaaacga aatgaactgg ctcaggcatc cgctaaatat
     aaagaactgg atgagctggt caaaaaacta tcaccaagag ccaatgatcc gcttcagaac
     cgtccttttt ttgaagcaac cagacgacgg gttggggccg gtaagattag agaagaaaaa
     caaaaacagg taacagcatc agaaacacgt attaaccgga taaatgctga tataactcag
     atccagaagg ctatttctca ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat
     gaagctgaag aaaatttgaa aaaagcacag aataatctcc ttaattcaca gattaaggat
     gctgttgatg caacagttag cttttatcaa acgctgactg aaaaatatgg tgaaaaatat
     tcgaaaatgg cacaggaact tgctgataag tctaaaggta agaaaatcgg caatgtgaat
     gaagctctcg ctgcttttga aaaatacaag gatgttttaa ataagaaatt cagcaaagcc
     gatcgtgatg ctatttttaa tgcgttggca tcggtgaagt atgatgactg ggctaaacat
     ttagatcagt ttgccaagta cttgaagatt acggggcatg tttcttttgg atatgatgtg
     gtatctgata tcctaaaaat taaggataca ggtgactgga agccactatt tcttacatta
     gagaagaaag ctgcagatgc aggggtgagt tatgttgttg ctttactttt tagcttgctt
     gctggaacta cattaggtat ttggggtatt gctattgtta caggaa
//