Return to this vector's summary.
ID EMBL3RTARM preliminary; circular DNA; SYN; 9170 BP.
XX
AC U02425; U02453; X58667; X58668; X06780; IG1003; ATCC37266;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector lambda EMBL3 right arm - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC EMBL1 from lambda 1059 & pBR322
RC EMBL2 from EMBL1
RC EMBL2Sal, EMBL2RI from EMBL2
RC [pUC2 from M13mp2]
RC pUC(S-Bg), pUC(R-Bg) from pUC2
RC EMBL3 from EMBL2Sal & EMBL2RI & pUC(S-Bg)
RC EMBL4 from EMBL2Sal & EMBL2RI & pUC(R-Bg)
RC EMBL3AamBam, EMBL3Sam7, EMBL3Aam, EMBL3Bam from EMBL3
RC EMBL3AamSam, EMBL3BamSam from EMBL3
RA Frischauf A.M., Lehrach H., Poustka A., Murray N.E.;
RT "Lambda replacement vectors carrying polylinker sequences";
RL J. Mol. Biol. 170:827-842(1983).
XX
RN [2]
RC EMBL3, polylinker
RC EMBL4, polylinker
RA Goedert M., Rogers J., Wilson P.W.;
RT ;
RL Submitted (08-FEB-1988) to GenBank by:
RL Goedert M., Rogers J., Wilson P.W.,
RL Department of Physiology, University of Cambridge, Downing Street,
RL Cambridge CB2 3EG, UNITED KINGDOM.
XX
RN [3]
RC EMBL3, polylinker
RC EMBL4, polylinker
RA Schulman A.H.;
RT ;
RL Submitted (27-MAR-1991) to GenBank by:
RL Schulman A.H., Institute of Biotechnology, University of Helsinki,
RL Karvaamokuja 3, SF-00380 Helsinki, FINLAND.
XX
RN [4]
RP 1-20067, 1-9170
RC EMBL3 right/left arm
RA Kitts P.A.;
RT "CLONTECH Vectors On Disc version 1.1";
RL Unpublished (1993).
XX
RN [5]
RP 1-20067, 1-9170
RC EMBL3 right/left arm
RA Kitts P.A.;
RT ;
RL Submitted (07-OCT-1993) by:
RL Kitts P.A., CLONTECH Laboratories, Inc.,
RL 4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
CC EMBL3 size is between 43000 and 44000 bp.
CC left arm is 19300. right arm is 9200 bp. The stuffer is 14000 bp.
CC Phage with inserts have the Spi- phenotype. [3]
CC lambdaEMBL3 (ATCC 37266) and lambdaEMBL4 (ATCC 37267) differ only in
CC the orientation of the MCS surrounding the stuffer fragment.
CC Medium is 1592 SM buffer.
CC This sequence has been compiled from information in the sequence
CC databases, published literature and other sources; this vector has
CC not been completely sequenced. If you suspect there is an error in
CC this sequence, please contact CLONTECH's Technical Service
CC Department at (415) 424-8222 or (800) 662-2566, extension 3 or
CC E-mail CLONTECH@BIOTECHNET.COM.
CC NCBI gi: 413791
CC NCBI gi: 413819
CC NM (lambda EMBL3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)(linear)
CC ST ()
CC TY (phage)
CC SP (CLONTECH)(Promega)(Stratagene)(ATCC)
CC HO (E.coli LE392)(E.coli O358)(E.coli O359)(E.coli K803)
CC HO (E.coli NM538)(E.coli NM539)(E.coli KW251)
CC HO (E.coli XL-1-Blue MRA)(E.coli XL-1-Blue MRA P2)
CC CP ()
CC FN (cloning 8000-23000 bp)(chromosome walking)
CC SE ()
CC PA (lambda 1059)
CC BR (EMBL4)
CC OF ()
CC OR (E.coli Y1090)
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda 1059 remove middle HindIII-HindIII
FT \ 21011bp, lambda 23131..44142/27491bp
FT \ lambda 23131 25158 27480 36896 37460 44142
FT 2. E. coli HindIII-HindIII 5700bp, trpE gene
FT -> EMBL1 33200bp
FT 1. EMBL1 EcoRI, lambda 21227
FT \ lambda 21227 26105 31748 39169 44973
FT mutation
FT EcoRI, lambda 44973
FT mutation
FT -> EMBL2 33200bp [no EcoRI]
FT 1. M13mp2, MCS
FT -> pUC2
FT 1. pUC2 EcoRI 2200bp, pUC4 327 or 369
FT EcoRI-BglII-EcoRI linker 18bp gaattcagatctgaattc
FT EcoRI-EcoRI
FT -> pUC(R-Bg) 2200bp [EcoRI-BglII-EcoRI]
FT 1. pUC2 SalI 2200bp, pUC4 342 or 354
FT SalI-BglII-SalI linker 18bp gtcgacagatctgtcgac
FT SalI-SalI
FT -> pUC(S-Bg) 2200bp
FT \ [EcoRI-BamHI-SalI-BglII-SalI-BamHI-EcoRI]
FT 1. EMBL2 remove small BamHI-BamHI
FT \ 16841bp lambda 5506..22347/16359bp
FT \ lambda 5506 22347 27973 34500 41733
FT 2. pUC(R-Bg) BglII 2200bp
FT -> EMBLRI
FT 1. EMBL2 remove small BamHI-BamHI
FT \ 16841bp lambda 5506..22347/16359bp
FT \ lambda 5506 22347 27973 34500 41733
FT 2. pUC(S-Bg) BglII 2200bp
FT -> EMBLSal
FT 1. EMBLRI remove middle EcoRI-EcoRI 2200bp
FT 2. E. coli EcoRI-EcoRI
FT -> phage
FT 1. EMBLSal remove middle EcoRI-EcoRI 2200bp
FT 2. E. coli EcoRI-EcoRI
FT -> phage2
FT 1. EMBLRI middle EcoRI-EcoRI 2200bp
FT 2. phage2 remove middle EcoRI-EcoRI, 27000bp
FT -> EMBL3 29237bp
FT 1. EMBLSal middle EcoRI-EcoRI 2200bp
FT 2. phage remove middle EcoRI-EcoRI, 40800bp
FT -> EMBL4 43000bp"
FT misc_feature 0..0
FT /note="sequence to the left and right of polylinker"
FT misc_binding 0..0
FT /note="MCS double SalI-BamHI-EcoRI-EcoRI-BamHI-SalI
FT ggatctgggtcgacctgcaggtcaacggatccgttgacctgcaggtcgac
FT ccagatctgggtcgaccggtcgacccagatcc"
FT misc_binding 0..0
FT /note="SIT EcoRI-EcoRI, left and right arms,
FT ggatctgggtcgacctgcaggtcaacg gatccgttgacctgcaggtcgac
FT ccagatctgggtcgaccggtcgacccagatcc"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT misc_binding 0..0
FT /note="SIT KpnI"
FT misc_binding 0..0
FT /note="SIT KpnI"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT misc_feature 0..0
FT /note="lambda 2001 stuffer fragment (13700 bp)"
FT CDS 0..0
FT /note="GEN bacteriophage lambda red+"
FT CDS 0..0
FT /note="GEN bacteriophage lambda gam+"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT misc_binding 0..0
FT /note="SIT SalI"
FT misc_binding 0..0
FT /note="SIT SalI"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT misc_binding 0..0
FT /note="SIT SstI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT CDS 0..0
FT /note="GEN bacteriophage lambda Spi+ reporter gene"
XX
SQ Sequence 9170 BP; 2534 A; 1996 C; 2251 G; 2389 T; 0 other;
ggatccgttg acctgcaggt cgacccagat ctgggtcgac cggtcgaccc agatccactc
gttattctcg gacgagtgtt cagtaatgaa cctctggaga gaaccatgta tatgatcgtt
atctgggttg gacttctgct tttaagccca gataactggc ctgaatatgt taatgagaga
atcggtattc ctcatgtgtg gcatgttttc gtctttgctc ttgcattttc gctagcaatt
aatgtgcatc gattatcagc tattgccagc gccagatata agcgatttaa gctaagaaaa
cgcattaaga tgcaaaacga taaagtgcga tcagtaattc aaaaccttac agaagagcaa
tctatggttt tgtgcgcagc ccttaatgaa ggcaggaagt atgtggttac atcaaaacaa
ttcccataca ttagtgagtt gattgagctt ggtgtgttga acaaaacttt ttcccgatgg
aatggaaagc atatattatt ccctattgag gatatttact ggactgaatt agttgccagc
tatgatccat ataatattga gataaagcca aggccaatat ctaagtaact agataagagg
aatcgatttt cccttaattt tctggcgtcc actgcatgtt atgccgcgtt cgccaggctt
gctgtaccat gtgcgctgat tcttgcgctc aatacgttgc aggttgcttt caatctgttt
gtggtattca gccagcactg taaggtctat cggatttagt gcgctttcta ctcgtgattt
cggtttgcga ttcagcgaga gaatagggcg gttaactggt tttgcgctta ccccaaccaa
caggggattt gctgctttcc attgagcctg tttctctgcg cgacgttcgc ggcggcgtgt
ttgtgcatcc atctggattc tcctgtcagt tagctttggt ggtgtgtggc agttgtagtc
ctgaacgaaa accccccgcg attggcacat tggcagctaa tccggaatcg cacttacggc
caatgcttcg tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc
agggcttaat ttttaagagc gtcaccttca tggtggtcag tgcgtcctgc tgatgtgctc
agtatcaccg ccagtggtat ttatgtcaac accgccagag ataatttatc accgcagatg
gttatctgta tgttttttat atgaatttat tttttgcagg ggggcattgt ttggtaggtg
agagatctga attgctatgt ttagtgagtt gtatctattt atttttcaat aaatacaatt
ggttatgtgt tttgggggcg atcgtgaggc aaagaaaacc cggcgctgag gccgggttat
tcttgttctc tggtcaaatc ttttttgtgc tcatacgtta aatctatcac cgcaagggat
aaatatctaa caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgcatgt
actaaggagg ttgtatggaa caacgcataa ccctgaaaga ttatgcaatg cgctttgggc
aaaccaagac agctaaagat ctcggcgtat atcaaagcgc gatcaacaag gccattcatg
caggccgaaa gattttttta actataaacg ctgatggaag cgtttatgcg gaagaggtaa
agcccttccc gagtaacaaa aaaacaacag cataaataac cccgctctta cacattccag
ccctgaaaaa gggcatcaaa ttaaaccaca cctatggtgt atgcatttat ttgcatacat
tcaatcaatt gttatctaag gaaatactta catatggttc gtgcaaacaa acgcaacgag
gctctacgaa tcgagagtgc gttgcttaac aaaatcgcaa tgcttggaac tgagaagaca
gcggaagctg tgggcgttga taagtcgcag atcagctggt ggaagaggga ctggattcca
aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg ttgacgacga catggctcga
ttggcgcgac aagttgctgc gattctcacc aataaaaaac gcccggcggc aaccgagcgt
tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtcatta
tgacaaatac agcaaaaata ctcaacttcg gcagaggtaa ctttgccgga caggagcgta
atgtggcaga tctcgatgat ggttacgcca gactatcaaa tatgctgctt gaggcttatt
cgggcgcaga tctgaccaag cgacagttta aagtgctgct tgccattctg cgtaaaacct
atgggtggaa taaaccaatg gacagaatca ccgattctca acttagcgag attacaaagt
tacctgtcaa acggtgcaat gaagccaagt tagaactcgt cagaatgaat attatcaagc
agcaaggcgg catgtttgga ccaaataaaa acatctcaga atggtgcatc cctcaaaacg
agggaaaatc ccctaaaacg agggataaaa catccctcaa attgggggat tgctatccct
caaaacaggg ggacacaaaa gacactatta caaaagaaaa aagaaaagat tattcgtcag
agaactctgg cgaatcctct gaccagccag aaaacgacct ttctgtggtg aaaccggatg
ctgcaattca gagcggcagc aagtggggga cagcagaaga cctgaccgcc gcagagtgga
tgtttgacat ggtgaagact atcgcaccat cagccagaaa accgaatttt gctgggtggg
ctaacgatat ccgcctgatg cgtgaacgtg acggacgtaa ccaccgcgac atgtgtgtgc
tgttccgctg ggcatgccag gacaacttct ggtccggtaa cgtgctgagc ccggccaaac
tccgcgataa gtggacccaa ctcgaaatca accgtaacaa gcaacaggca ggcgtgacag
ccagcaaacc aaaactcgac ctgacaaaca cagactggat ttacggggtg gatctatgaa
aaacatcgcc gcacagatgg ttaactttga ccgtgagcag atgcgtcgga tcgccaacaa
catgccggaa cagtacgacg aaaagccgca ggtacagcag gtagcgcaga tcatcaacgg
tgtgttcagc cagttactgg caactttccc ggcgagcctg gctaaccgtg accagaacga
agtgaacgaa atccgtcgcc agtgggttct ggcttttcgg gaaaacggga tcaccacgat
ggaacaggtt aacgcaggaa tgcgcgtagc ccgtcggcag aatcgaccat ttctgccatc
acccgggcag tttgttgcat ggtgccggga agaagcatcc gttaccgccg gactgccaaa
cgtcagcgag ctggttgata tggtttacga gtattgccgg aagcgaggcc tgtatccgga
tgcggagtct tatccgtgga aatcaaacgc gcactactgg ctggttacca acctgtatca
gaacatgcgg gccaatgcgc ttactgatgc ggaattacgc cgtaaggccg cagatgagct
tgtccatatg actgcgagaa ttaaccgtgg tgaggcgatc cctgaaccag taaaacaact
tcctgtcatg ggcggtagac ctctaaatcg tgcacaggct ctggcgaaga tcgcagaaat
caaagctaag ttcggactga aaggagcaag tgtatgacgg gcaaagaggc aattattcat
tacctgggga cgcataatag cttctgtgcg ccggacgttg ccgcgctaac aggcgcaaca
gtaaccagca taaatcaggc cgcggctaaa atggcacggg caggtcttct ggttatcgaa
ggtaaggtct ggcgaacggt gtattaccgg tttgctacca gggaagaacg ggaaggaaag
atgagcacga acctgatgaa caaactggat acgattggat tcgacaacaa aaaagacctg
cttatctcgg tgggcgattt ggttgatcgt ggtgcagaga acgttgaatg cctggaatta
atcacattcc cctggttcag agctgtacgt ggaaaccatg agcaaatgat gattgatggc
ttatcagagc gtggaaacgt taatcactgg ctgcttaatg gcggtggctg gttctttaat
ctcgattacg acaaagaaat tctggctaaa gctcttgccc ataaagcaga tgaacttccg
ttaatcatcg aactggtgag caaagataaa aaatatgtta tctgccacgc cgattatccc
tttgacgaat acgagtttgg aaagccagtt gatcatcagc aggtaatctg gaaccgcgaa
cgaatcagca actcacaaaa cgggatcgtg aaagaaatca aaggcgcgga cacgttcatc
tttggtcata cgccagcagt gaaaccactc aagtttgcca accaaatgta tatcgatacc
ggcgcagtgt tctgcggaaa cctaacattg attcaggtac agggagaagg cgcatgagac
tcgaaagcgt agctaaattt cattcgccaa aaagcccgat gatgagcgac tcaccacggg
ccacggcttc tgactctctt tccggtactg atgtgatggc tgctatgggg atggcgcaat
cacaagccgg attcggtatg gctgcattct gcggtaagca cgaactcagc cagaacgaca
aacaaaaggc tatcaactat ctgatgcaat ttgcacacaa ggtatcgggg aaataccgtg
gtgtggcaaa gcttgaagga aatactaagg caaaggtact gcaagtgctc gcaacattcg
cttatgcgga ttattgccgt agtgccgcga cgccgggggc aagatgcaga gattgccatg
gtacaggccg tgcggttgat attgccaaaa cagagctgtg ggggagagtt gtcgagaaag
agtgcggaag atgcaaaggc gtcggctatt caaggatgcc agcaagcgca gcatatcgcg
ctgtgacgat gctaatccca aaccttaccc aacccacctg gtcacgcact gttaagccgc
tgtatgacgc tctggtggtg caatgccaca aagaagagtc aatcgcagac aacattttga
atgcggtcac acgttagcag catgattgcc acggatggca acatattaac ggcatgatat
tgacttattg aataaaattg ggtaaatttg actcaacgat gggttaattc gctcgttgtg
gtagtgagat gaaaagaggc ggcgcttact accgattccg cctagttggt cacttcgacg
tatcgtctgg aactccaacc atcgcaggca gagaggtctg caaaatgcaa tcccgaaaca
gttcgcaggt aatagttaga gcctgcataa cggtttcggg attttttata tctgcacaac
aggtaagagc attgagtcga taatcgtgaa gagtcggcga gcctggttag ccagtgctct
ttccgttgtg ctgaattaag cgaataccgg aagcagaacc ggatcaccaa atgcgtacag
gcgtcatcgc cgcccagcaa cagcacaacc caaactgagc cgtagccact gtctgtcctg
aactcattag taatagttac gctgcggcct tttacacatg accttcgtga aagcgggtgg
caggaggtcg cgctaacaac ctcctgccgt tttgcccgtg catatcggtc acgaacaaat
ctgattacta aacacagtag cctggatttg ttctatcagt aatcgacctt attcctaatt
aaatagagca aatcccctta ttgggggtaa gacatgaaga tgccagaaaa acatgacctg
ttggccgcca ttctcgcggc aaaggaacaa ggcatcgggg caatccttgc gtttgcaatg
gcgtaccttc gcggcagata taatggcggt gcgtttacaa aaacagtaat cgacgcaacg
atgtgcgcca ttatcgcctg gttcattcgt gaccttctcg acttcgccgg actaagtagc
aatctcgctt atataacgag cgtgtttatc ggctacatcg gtactgactc gattggttcg
cttatcaaac gcttcgctgc taaaaaagcc ggagtagaag atggtagaaa tcaataatca
acgtaaggcg ttcctcgata tgctggcgtg gtcggaggga actgataacg gacgtcagaa
aaccagaaat catggttatg acgtcattgt aggcggagag ctatttactg attactccga
tcaccctcgc aaacttgtca cgctaaaccc aaaactcaaa tcaacaggcg ccggacgcta
ccagcttctt tcccgttggt gggatgccta ccgcaagcag cttggcctga aagacttctc
tccgaaaagt caggacgctg tggcattgca gcagattaag gagcgtggcg ctttacctat
gattgatcgt ggtgatatcc gtcaggcaat cgaccgttgc agcaatatct gggcttcact
gccgggcgct ggttatggtc agttcgagca taaggctgac agcctgattg caaaattcaa
agaagcgggc ggaacggtca gagagattga tgtatgagca gagtcaccgc gattatctcc
gctctggtta tctgcatcat cgtctgcctg tcatgggctg ttaatcatta ccgtgataac
gccattacct acaaagccca gcgcgacaaa aatgccagag aactgaagct ggcgaacgcg
gcaattactg acatgcagat gcgtcagcgt gatgttgctg cgctcgatgc aaaatacacg
aaggagttag ctgatgctaa agctgaaaat gatgctctgc gtgatgatgt tgccgctggt
cgtcgtcggt tgcacatcaa agcagtctgt cagtcagtgc gtgaagccac caccgcctcc
ggcgtggata atgcagcctc cccccgactg gcagacaccg ctgaacggga ttatttcacc
ctcagagaga ggctgatcac tatgcaaaaa caactggaag gaacccagaa gtatattaat
gagcagtgca gatagagttg cccatatcga tgggcaactc atgcaattat tgtgagcaat
acacacgcgc ttccagcgga gtataaatgc ctaaagtaat aaaaccgagc aatccattta
cgaatgtttg ctgggtttct gttttaacaa cattttctgc gccgccacaa attttggctg
catcgacagt tttcttctgc ccaattccag aaacgaagaa atgatgggtg atggtttcct
ttggtgctac tgctgccggt ttgttttgaa cagtaaacgt ctgttgagca catcctgtaa
taagcagggc cagcgcagta gcgagtagca tttttttcat ggtgttattc ccgatgcttt
ttgaagttcg cagaatcgta tgtgtagaaa attaaacaaa ccctaaacaa tgagttgaaa
tttcatattg ttaatattta ttaatgtatg tcaggtgcga tgaatcgtca ttgtattccc
ggattaacta tgtccacagc cctgacgggg aacttctctg cgggagtgtc cgggaataat
taaaacgatg cacacagggt ttagcgcgta cacgtattgc attatgccaa cgccccggtg
ctgacacgga agaaaccgga cgttatgatt tagcgtggaa agatttgtgt agtgttctga
atgctctcag taaatagtaa tgaattatca aaggtatagt aatatctttt atgttcatgg
atatttgtaa cccatcggaa aactcctgct ttagcaagat tttccctgta ttgctgaaat
gtgatttctc ttgatttcaa cctatcatag gacgtttcta taagatgcgt gtttcttgag
aatttaacat ttacaacctt tttaagtcct tttattaaca cggtgttatc gttttctaac
acgatgtgaa tattatctgt ggctagatag taaatataat gtgagacgtt gtgacgtttt
agttcagaat aaaacaattc acagtctaaa tcttttcgca cttgatcgaa tatttcttta
aaaatggcaa cctgagccat tggtaaaacc ttccatgtga tacgagggcg cgtagtttgc
attatcgttt ttatcgtttc aatctggtct gacctccttg tgttttgttg atgatttatg
tcaaatatta ggaatgtttt cacttaatag tattggttgc gtaacaaagt gcggtcctgc
tggcattctg gagggaaata caaccgacag atgtatgtaa ggccaacgtg ctcaaatctt
catacagaaa gatttgaagt aatattttaa ccgctagatg aagagcaagc gcatggagcg
acaaaatgaa taaagaacaa tctgctgatg atccctccgt ggatctgatt cgtgtaaaaa
atatgcttaa tagcaccatt tctatgagtt accctgatgt tgtaattgca tgtatagaac
ataaggtgtc tctggaagca ttcagagcaa ttgaggcagc gttggtgaag cacgataata
atatgaagga ttattccctg gtggttgact gatcaccata actgctaatc attcaaacta
tttagtctgt gacagagcca acacgcagtc tgtcactgtc aggaaagtgg taaaactgca
actcaattac tgcaatgccc tcgtaattaa gtgaatttac aatatcgtcc tgttcggagg
gaagaacgcg ggatgttcat tcttcatcac ttttaattga tgtatatgct ctcttttctg
acgttagtct ccgacggcag gcttcaatga cccaggctga gaaattcccg gacccttttt
gctcaagagc gatgttaatt tgttcaatca tttggttagg aaagcggatg ttgcgggttg
ttgttctgcg ggttctgttc ttcgttgaca tgaggttgcc ccgtattcag tgtcgctgat
ttgtattgtc tgaagttgtt tttacgttaa gttgatgcag atcaattaat acgatacctg
cgtcataatt gattatttga cgtggtttga tggcctccac gcacgttgtg atatgtagat
gataatcatt atcactttac gggtcctttc cggtgatccg acaggttacg
//