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ID   EMBL3S6T7R preliminary; circular DNA; SYN; 9205 BP.
XX
AC   U02427;
XX
DT   08-NOV-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector lambda EMBL3 SP6/T7 right arm - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-9205
RC   EMBL3 SP6/T7 right arm
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.1";
RL   Unpublished (1993).
XX
RN   [2]
RP   1-9205
RC   EMBL3 SP6/T7 right arm
RA   Kitts P.A.;
RT   ;
RL   Submitted (07-OCT-1993) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
CC   This sequence has been compiled from information in the sequence
CC   databases, published literature and other sources; this vector has
CC   not been completely sequenced. If you suspect there is an error in
CC   this sequence, please contact CLONTECH's Technical Service
CC   Department at (415) 424-8222 or (800) 662-2566, extension 3 or
CC   E-mail CLONTECH@BIOTECHNET.COM.
CC   NCBI gi: 413793
CC   NM (EMBL3 SP6/T7 right arm)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (EMBL3)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. EMBL3 29237bp
FT                   2. Sp6 promoter
FT                   3. T7 promoter
FT                   -> EMBL3 SP6/T7 29330bp"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
XX
SQ   Sequence 9205 BP; 2546 A; 2002 C; 2253 G; 2404 T; 0 other;
     tctagaagct tgtcgacgaa ttcctaggat cctcgagctc ttctatagtg tcacctaaat
     ggccatttag gcctagagtc gacccagatc cactcgttat tctcggacga gtgttcagta
     atgaacctct ggagagaacc atgtatatga tcgttatctg ggttggactt ctgcttttaa
     gcccagataa ctggcctgaa tatgttaatg agagaatcgg tattcctcat gtgtggcatg
     ttttcgtctt tgctcttgca ttttcgctag caattaatgt gcatcgatta tcagctattg
     ccagcgccag atataagcga tttaagctaa gaaaacgcat taagatgcaa aacgataaag
     tgcgatcagt aattcaaaac cttacagaag agcaatctat ggttttgtgc gcagccctta
     atgaaggcag gaagtatgtg gttacatcaa aacaattccc atacattagt gagttgattg
     agcttggtgt gttgaacaaa actttttccc gatggaatgg aaagcatata ttattcccta
     ttgaggatat ttactggact gaattagttg ccagctatga tccatataat attgagataa
     agccaaggcc aatatctaag taactagata agaggaatcg attttccctt aattttctgg
     cgtccactgc atgttatgcc gcgttcgcca ggcttgctgt accatgtgcg ctgattcttg
     cgctcaatac gttgcaggtt gctttcaatc tgtttgtggt attcagccag cactgtaagg
     tctatcggat ttagtgcgct ttctactcgt gatttcggtt tgcgattcag cgagagaata
     gggcggttaa ctggttttgc gcttacccca accaacaggg gatttgctgc tttccattga
     gcctgtttct ctgcgcgacg ttcgcggcgg cgtgtttgtg catccatctg gattctcctg
     tcagttagct ttggtggtgt gtggcagttg tagtcctgaa cgaaaacccc ccgcgattgg
     cacattggca gctaatccgg aatcgcactt acggccaatg cttcgtttcg tatcacacac
     cccaaagcct tctgctttga atgctgccct tcttcagggc ttaattttta agagcgtcac
     cttcatggtg gtcagtgcgt cctgctgatg tgctcagtat caccgccagt ggtatttatg
     tcaacaccgc cagagataat ttatcaccgc agatggttat ctgtatgttt tttatatgaa
     tttatttttt gcaggggggc attgtttggt aggtgagaga tctgaattgc tatgtttagt
     gagttgtatc tatttatttt tcaataaata caattggtta tgtgttttgg gggcgatcgt
     gaggcaaaga aaacccggcg ctgaggccgg gttattcttg ttctctggtc aaatcttttt
     tgtgctcata cgttaaatct atcaccgcaa gggataaata tctaacaccg tgcgtgttga
     ctattttacc tctggcggtg ataatggttg catgtactaa ggaggttgta tggaacaacg
     cataaccctg aaagattatg caatgcgctt tgggcaaacc aagacagcta aagatctcgg
     cgtatatcaa agcgcgatca acaaggccat tcatgcaggc cgaaagattt ttttaactat
     aaacgctgat ggaagcgttt atgcggaaga ggtaaagccc ttcccgagta acaaaaaaac
     aacagcataa ataaccccgc tcttacacat tccagccctg aaaaagggca tcaaattaaa
     ccacacctat ggtgtatgca tttatttgca tacattcaat caattgttat ctaaggaaat
     acttacatat ggttcgtgca aacaaacgca acgaggctct acgaatcgag agtgcgttgc
     ttaacaaaat cgcaatgctt ggaactgaga agacagcgga agctgtgggc gttgataagt
     cgcagatcag ctggtggaag agggactgga ttccaaagtt ctcaatgctg cttgctgttc
     ttgaatgggg ggtcgttgac gacgacatgg ctcgattggc gcgacaagtt gctgcgattc
     tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     gaggtcatta ctggatctat caacaggagt cattatgaca aatacagcaa aaatactcaa
     cttcggcaga ggtaactttg ccggacagga gcgtaatgtg gcagatctcg atgatggtta
     cgccagacta tcaaatatgc tgcttgaggc ttattcgggc gcagatctga ccaagcgaca
     gtttaaagtg ctgcttgcca ttctgcgtaa aacctatggg tggaataaac caatggacag
     aatcaccgat tctcaactta gcgagattac aaagttacct gtcaaacggt gcaatgaagc
     caagttagaa ctcgtcagaa tgaatattat caagcagcaa ggcggcatgt ttggaccaaa
     taaaaacatc tcagaatggt gcatccctca aaacgaggga aaatccccta aaacgaggga
     taaaacatcc ctcaaattgg gggattgcta tccctcaaaa cagggggaca caaaagacac
     tattacaaaa gaaaaaagaa aagattattc gtcagagaac tctggcgaat cctctgacca
     gccagaaaac gacctttctg tggtgaaacc ggatgctgca attcagagcg gcagcaagtg
     ggggacagca gaagacctga ccgccgcaga gtggatgttt gacatggtga agactatcgc
     accatcagcc agaaaaccga attttgctgg gtgggctaac gatatccgcc tgatgcgtga
     acgtgacgga cgtaaccacc gcgacatgtg tgtgctgttc cgctgggcat gccaggacaa
     cttctggtcc ggtaacgtgc tgagcccggc caaactccgc gataagtgga cccaactcga
     aatcaaccgt aacaagcaac aggcaggcgt gacagccagc aaaccaaaac tcgacctgac
     aaacacagac tggatttacg gggtggatct atgaaaaaca tcgccgcaca gatggttaac
     tttgaccgtg agcagatgcg tcggatcgcc aacaacatgc cggaacagta cgacgaaaag
     ccgcaggtac agcaggtagc gcagatcatc aacggtgtgt tcagccagtt actggcaact
     ttcccggcga gcctggctaa ccgtgaccag aacgaagtga acgaaatccg tcgccagtgg
     gttctggctt ttcgggaaaa cgggatcacc acgatggaac aggttaacgc aggaatgcgc
     gtagcccgtc ggcagaatcg accatttctg ccatcacccg ggcagtttgt tgcatggtgc
     cgggaagaag catccgttac cgccggactg ccaaacgtca gcgagctggt tgatatggtt
     tacgagtatt gccggaagcg aggcctgtat ccggatgcgg agtcttatcc gtggaaatca
     aacgcgcact actggctggt taccaacctg tatcagaaca tgcgggccaa tgcgcttact
     gatgcggaat tacgccgtaa ggccgcagat gagcttgtcc atatgactgc gagaattaac
     cgtggtgagg cgatccctga accagtaaaa caacttcctg tcatgggcgg tagacctcta
     aatcgtgcac aggctctggc gaagatcgca gaaatcaaag ctaagttcgg actgaaagga
     gcaagtgtat gacgggcaaa gaggcaatta ttcattacct ggggacgcat aatagcttct
     gtgcgccgga cgttgccgcg ctaacaggcg caacagtaac cagcataaat caggccgcgg
     ctaaaatggc acgggcaggt cttctggtta tcgaaggtaa ggtctggcga acggtgtatt
     accggtttgc taccagggaa gaacgggaag gaaagatgag cacgaacctg atgaacaaac
     tggatacgat tggattcgac aacaaaaaag acctgcttat ctcggtgggc gatttggttg
     atcgtggtgc agagaacgtt gaatgcctgg aattaatcac attcccctgg ttcagagctg
     tacgtggaaa ccatgagcaa atgatgattg atggcttatc agagcgtgga aacgttaatc
     actggctgct taatggcggt ggctggttct ttaatctcga ttacgacaaa gaaattctgg
     ctaaagctct tgcccataaa gcagatgaac ttccgttaat catcgaactg gtgagcaaag
     ataaaaaata tgttatctgc cacgccgatt atccctttga cgaatacgag tttggaaagc
     cagttgatca tcagcaggta atctggaacc gcgaacgaat cagcaactca caaaacggga
     tcgtgaaaga aatcaaaggc gcggacacgt tcatctttgg tcatacgcca gcagtgaaac
     cactcaagtt tgccaaccaa atgtatatcg ataccggcgc agtgttctgc ggaaacctaa
     cattgattca ggtacaggga gaaggcgcat gagactcgaa agcgtagcta aatttcattc
     gccaaaaagc ccgatgatga gcgactcacc acgggccacg gcttctgact ctctttccgg
     tactgatgtg atggctgcta tggggatggc gcaatcacaa gccggattcg gtatggctgc
     attctgcggt aagcacgaac tcagccagaa cgacaaacaa aaggctatca actatctgat
     gcaatttgca cacaaggtat cggggaaata ccgtggtgtg gcaaagcttg aaggaaatac
     taaggcaaag gtactgcaag tgctcgcaac attcgcttat gcggattatt gccgtagtgc
     cgcgacgccg ggggcaagat gcagagattg ccatggtaca ggccgtgcgg ttgatattgc
     caaaacagag ctgtggggga gagttgtcga gaaagagtgc ggaagatgca aaggcgtcgg
     ctattcaagg atgccagcaa gcgcagcata tcgcgctgtg acgatgctaa tcccaaacct
     tacccaaccc acctggtcac gcactgttaa gccgctgtat gacgctctgg tggtgcaatg
     ccacaaagaa gagtcaatcg cagacaacat tttgaatgcg gtcacacgtt agcagcatga
     ttgccacgga tggcaacata ttaacggcat gatattgact tattgaataa aattgggtaa
     atttgactca acgatgggtt aattcgctcg ttgtggtagt gagatgaaaa gaggcggcgc
     ttactaccga ttccgcctag ttggtcactt cgacgtatcg tctggaactc caaccatcgc
     aggcagagag gtctgcaaaa tgcaatcccg aaacagttcg caggtaatag ttagagcctg
     cataacggtt tcgggatttt ttatatctgc acaacaggta agagcattga gtcgataatc
     gtgaagagtc ggcgagcctg gttagccagt gctctttccg ttgtgctgaa ttaagcgaat
     accggaagca gaaccggatc accaaatgcg tacaggcgtc atcgccgccc agcaacagca
     caacccaaac tgagccgtag ccactgtctg tcctgaactc attagtaata gttacgctgc
     ggccttttac acatgacctt cgtgaaagcg ggtggcagga ggtcgcgcta acaacctcct
     gccgttttgc ccgtgcatat cggtcacgaa caaatctgat tactaaacac agtagcctgg
     atttgttcta tcagtaatcg accttattcc taattaaata gagcaaatcc ccttattggg
     ggtaagacat gaagatgcca gaaaaacatg acctgttggc cgccattctc gcggcaaagg
     aacaaggcat cggggcaatc cttgcgtttg caatggcgta ccttcgcggc agatataatg
     gcggtgcgtt tacaaaaaca gtaatcgacg caacgatgtg cgccattatc gcctggttca
     ttcgtgacct tctcgacttc gccggactaa gtagcaatct cgcttatata acgagcgtgt
     ttatcggcta catcggtact gactcgattg gttcgcttat caaacgcttc gctgctaaaa
     aagccggagt agaagatggt agaaatcaat aatcaacgta aggcgttcct cgatatgctg
     gcgtggtcgg agggaactga taacggacgt cagaaaacca gaaatcatgg ttatgacgtc
     attgtaggcg gagagctatt tactgattac tccgatcacc ctcgcaaact tgtcacgcta
     aacccaaaac tcaaatcaac aggcgccgga cgctaccagc ttctttcccg ttggtgggat
     gcctaccgca agcagcttgg cctgaaagac ttctctccga aaagtcagga cgctgtggca
     ttgcagcaga ttaaggagcg tggcgcttta cctatgattg atcgtggtga tatccgtcag
     gcaatcgacc gttgcagcaa tatctgggct tcactgccgg gcgctggtta tggtcagttc
     gagcataagg ctgacagcct gattgcaaaa ttcaaagaag cgggcggaac ggtcagagag
     attgatgtat gagcagagtc accgcgatta tctccgctct ggttatctgc atcatcgtct
     gcctgtcatg ggctgttaat cattaccgtg ataacgccat tacctacaaa gcccagcgcg
     acaaaaatgc cagagaactg aagctggcga acgcggcaat tactgacatg cagatgcgtc
     agcgtgatgt tgctgcgctc gatgcaaaat acacgaagga gttagctgat gctaaagctg
     aaaatgatgc tctgcgtgat gatgttgccg ctggtcgtcg tcggttgcac atcaaagcag
     tctgtcagtc agtgcgtgaa gccaccaccg cctccggcgt ggataatgca gcctcccccc
     gactggcaga caccgctgaa cgggattatt tcaccctcag agagaggctg atcactatgc
     aaaaacaact ggaaggaacc cagaagtata ttaatgagca gtgcagatag agttgcccat
     atcgatgggc aactcatgca attattgtga gcaatacaca cgcgcttcca gcggagtata
     aatgcctaaa gtaataaaac cgagcaatcc atttacgaat gtttgctggg tttctgtttt
     aacaacattt tctgcgccgc cacaaatttt ggctgcatcg acagttttct tctgcccaat
     tccagaaacg aagaaatgat gggtgatggt ttcctttggt gctactgctg ccggtttgtt
     ttgaacagta aacgtctgtt gagcacatcc tgtaataagc agggccagcg cagtagcgag
     tagcattttt ttcatggtgt tattcccgat gctttttgaa gttcgcagaa tcgtatgtgt
     agaaaattaa acaaacccta aacaatgagt tgaaatttca tattgttaat atttattaat
     gtatgtcagg tgcgatgaat cgtcattgta ttcccggatt aactatgtcc acagccctga
     cggggaactt ctctgcggga gtgtccggga ataattaaaa cgatgcacac agggtttagc
     gcgtacacgt attgcattat gccaacgccc cggtgctgac acggaagaaa ccggacgtta
     tgatttagcg tggaaagatt tgtgtagtgt tctgaatgct ctcagtaaat agtaatgaat
     tatcaaaggt atagtaatat cttttatgtt catggatatt tgtaacccat cggaaaactc
     ctgctttagc aagattttcc ctgtattgct gaaatgtgat ttctcttgat ttcaacctat
     cataggacgt ttctataaga tgcgtgtttc ttgagaattt aacatttaca acctttttaa
     gtccttttat taacacggtg ttatcgtttt ctaacacgat gtgaatatta tctgtggcta
     gatagtaaat ataatgtgag acgttgtgac gttttagttc agaataaaac aattcacagt
     ctaaatcttt tcgcacttga tcgaatattt ctttaaaaat ggcaacctga gccattggta
     aaaccttcca tgtgatacga gggcgcgtag tttgcattat cgtttttatc gtttcaatct
     ggtctgacct ccttgtgttt tgttgatgat ttatgtcaaa tattaggaat gttttcactt
     aatagtattg gttgcgtaac aaagtgcggt cctgctggca ttctggaggg aaatacaacc
     gacagatgta tgtaaggcca acgtgctcaa atcttcatac agaaagattt gaagtaatat
     tttaaccgct agatgaagag caagcgcatg gagcgacaaa atgaataaag aacaatctgc
     tgatgatccc tccgtggatc tgattcgtgt aaaaaatatg cttaatagca ccatttctat
     gagttaccct gatgttgtaa ttgcatgtat agaacataag gtgtctctgg aagcattcag
     agcaattgag gcagcgttgg tgaagcacga taataatatg aaggattatt ccctggtggt
     tgactgatca ccataactgc taatcattca aactatttag tctgtgacag agccaacacg
     cagtctgtca ctgtcaggaa agtggtaaaa ctgcaactca attactgcaa tgccctcgta
     attaagtgaa tttacaatat cgtcctgttc ggagggaaga acgcgggatg ttcattcttc
     atcactttta attgatgtat atgctctctt ttctgacgtt agtctccgac ggcaggcttc
     aatgacccag gctgagaaat tcccggaccc tttttgctca agagcgatgt taatttgttc
     aatcatttgg ttaggaaagc ggatgttgcg ggttgttgtt ctgcgggttc tgttcttcgt
     tgacatgagg ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac
     gttaagttga tgcagatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg
     tttgatggcc tccacgcacg ttgtgatatg tagatgataa tcattatcac tttacgggtc
     ctttccggtg atccgacagg ttacg
//