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ID   FD478      preliminary; circular DNA; PHG; 6408 BP.
XX
AC   J02451; M10731; M10767; M21666; M21667; M21668; M21669; M21670;
AC   M25198;
XX
DT   22-FEB-1985 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector fd strain 478 - complete.
XX
KW   cloning vector.
XX
OS   Bacteriophage fd
OC   Viridae; ss-DNA nonenveloped viruses; Inoviridae.
XX
RN   [1]
RC   from phage fd, promoter
RA   Sugimoto K., Sugisaki H., Okamoto T., Takanami M.;
RT   "Studies on bacteriophage fd DNA. III. Nucleotide sequence preceding
RT   the RNA start-site on a promoter-containing fragment";
RL   Nucleic Acids Res. 2:2091-2100(1975).
XX
RN   [2]
RP   402-443
RC   phage fd from E. coli
RA   Schaller H., Gray C., Herrmann K.;
RT   "Nucleotide sequence of an RNA polymerase binding site from the DNA
RT   of bacteriophage fd";
RL   Proc. Natl. Acad. Sci. U.S.A. 72:737-741(1975).
XX
RN   [3]
RP   1196-1564
RC   from phage fd
RA   Sugimoto K., Sugisaki H., Okamoto T., Takanami M.;
RT   "Studies on bacteriophage fd DNA: IV The sequence of messenger RNA
RT   for the major coat protein gene";
RL   J. Mol. Biol. 110:487-507(1977).
XX
RN   [4]
RP   1-6408
RC   from phage fd
RA   Beck E., Sommer R., Auerswald E.A., Kurz C., Zink B.,
RA   Osterburg G., Schaller H., Sugimoto K., Sugisaki H., Okamoto T.,
RA   Takanami M.;
RT   "Nucleotide sequence of bacteriophage fd DNA";
RL   Nucleic Acids Res. 5:4495-4503(1978).
XX
RN   [5]
RP   5585-5771
RC   from phage fd
RA   Gray C., Sommer R., Polke C., Beck E., Schaller H.;
RT   "Structure of the origin of DNA replication of bacteriophage fd";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:50-53(1978).
XX
RN   [6]
RC   C3S::TnphoA from pJIH12 & phage f1 gene I fragments
RA   Horabin J.I., Webster R.E.;
RT   "An amino acid sequence which directs membrane insertion causes loss
RT   of membrane potential";
RL   J. Biol. Chem. 263:11575-11583(1988).
XX
RN   [7]
RC   pJIH1 from pKC30 & pGW7
RC   pJIH110, pJIH116 from pJIH1 & phage f1
RC   R6 from phage f1
RC   pJIH154 from pJIH1 & R6
RC   pJIH12 from pJIH1 & phage f1, gene I
RC   pJIH1215 from pJIH12 & phage f1, gene I
RC   pJIH13 from pACYC184 & pJIH1
RA   Horabin J.I., Webster R.E.;
RT   "Morphogenesis of f1 filamentous bacteriophage. Increased
RT   expression of gene I inhibits bacterial growth";
RL   J. Mol. Biol. 188:403-413(1986).
XX
CC   Sequence was compared with sumex tape.
CC   Replicative form is duplex.
CC   NM (bacteriophage fd strain 478)
CC   CM (yes)
CC   NA (filamentous ss-DNA)(ds-DNA)
CC   TP (linear)(circular)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (fd)
CC   BR ()
CC   OF ()
CC   OR (at HindII site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli
FT                   -> phage fd strain 478 6408bp"
FT   CDS             join(6007..6408,1..831)
FT                   /note="GEN bacteriophage fd II gene (rf replication,
FT                   nicking)"
FT   CDS             496..831
FT                   /note="GEN bacteriophage fd X gene (function unknown)"
FT   CDS             843..1106
FT                   /note="GEN bacteriophage fd V gene (single-stranded
FT                   binding protein)"
FT   CDS             1108..1209
FT                   /note="GEN bacteriophage fd VII gene (morphogenesis)"
FT   CDS             1206..1304
FT                   /note="GEN bacteriophage fd IX gene (minor coat
FT                   protein)"
FT   mRNA            <1301..1551
FT                   /note="bacteriophage fd mRNA (3' end +/- 14 bp)"
FT   CDS             1301..1522
FT                   /note="GEN bacteriophage fd VIII gene (major coat
FT                   protein)"
FT   CDS             1579..2853
FT                   /note="GEN bacteriophage fd III gene (adsorption
FT                   protein)"
FT   CDS             2856..3194
FT                   /note="GEN bacteriophage fd VI gene (morphogenesis)"
FT   CDS             3197..4243
FT                   /note="GEN bacteriophage fd I gene (morphogenesis)"
FT   misc_feature    3817..4081
FT                   /note="bacteriophage fd gene I internal signal
FT                   sequence [6]"
FT   CDS             4221..5501
FT                   /note="GEN bacteriophage fd IV gene (morphogenesis)"
FT   rep_origin      5782..5782
FT                   /note="ORI bacteriophage fd origin of viral strand
FT                   synthesis"
XX
SQ   Sequence 6408 BP; 1578 A; 1295 C; 1325 G; 2210 T; 0 other;
     aacgctacta ccattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
     atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
     cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
     gttgcatatt taaaacatgt tgaactacag caccagattc agcaattaag ctctaagcca
     tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactgtctaa tcctgacctg
     ttggaatttg cttccggtct ggttcgcttt gaggctcgaa ttgaaacgcg atatttgaag
     tctttcgggc ttcctcttaa tctttttgat gcaattcgct ttgcttctga ctataataga
     cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
     tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
     aaacatttta caattacccc ctctggcaaa acttcctttg caaaagcctc tcgctatttt
     ggtttctatc gtcgtctggt taatgagggt tatgatagtg ttgctcttac catgcctcgt
     aattcctttt ggcgttatgt atctgcatta gttgagtgtg gtattcctaa atctcaattg
     atgaatcttt ccacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
     tcctcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
     aaatgattaa agttgaaatt aaaccgtctc aagcgcaatt tactacccgt tctggtgttt
     ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
     aatatccggt gcttgtcaag attactctcg acgaaggtca gccagcgtat gcgcctggtc
     tgtacaccgt gcatctgtcc tcgttcaaag ttggtcagtt cggttctctt atgattgacc
     gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     caaagcctcc gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     cgatcccgca aaagcggcct ttgactccct gcaagcctca gcgaccgaat atatcggtta
     tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aacctcatac agaaaattca
     tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt
     ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg aaactcagtg ttacggtaca
     tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     cagaataata ggttccgaaa taggcagggt gcattaactg tttatacggg cactgttact
     caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
     gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt
     ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt
     gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     ttaatgaata atttccgtca atatttacct tctttgcctc agtcggttga atgtcgccct
     tatgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttcgacg
     tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttactttcc
     ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     ggcttaactc aattcttgtg ggttatctct ctgatattag cgcacaatta ccctctgatt
     ttgttcaggg cgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     attgggataa ataaatatgg ctgtttattt tgtaactggc aaattaggct ctggaaagac
     gctcgttagc gttggtaaga ttcaggataa aattgtagct gggtgcaaaa tagcaactaa
     tcttgattta aggcttcaaa acctcccgca agtcgggagg ttcgctaaaa cgcctcgcgt
     tcttagaata ccggataagc cttctatttc tgatttgctt gctattggtc gtggtaatga
     ttcctacgac gaaaataaaa acggtttgct tgttcttgat gaatgcggta cttggtttaa
     tacccgttca tggaatgaca aggaaagaca gccgattatt gattggtttc ttcatgctcg
     taaattggga tgggatatta tttttcttgt tcaggattta tctattgttg ataaacaggc
     gcgttctgca ttagctgaac acgttgttta ttgtcgccgt ctggacagaa ttactttacc
     ctttgtcggc actttatatt ctcttgttac tggctcaaaa atgcctctgc ctaaattaca
     tgttggtgtt gttaaatatg gtgattctca attaagccct actgttgagc gttggcttta
     tactggtaag aatttatata acgcatatga cactaaacag gctttttcca gtaattatga
     ttcaggtgtt tattcatatt taacccctta tttatcacac ggtcggtatt tcaaaccatt
     aaatttaggt cagaagatga aattaactaa aatatatttg aaaaagtttt ctcgcgttct
     ttgtcttgcg ataggatttg catcagcatt tacatatagt tatataaccc aacctaagcc
     ggaggttaaa aaggtagtct ctcagaccta tgattttgat aaattcacta ttgactcttc
     tcagcgtctt aatctaagct atcgctatgt tttcaaggat tctaagggaa aattaattaa
     tagcgacgat ttacagaagc aaggttattc catcacatat attgatttat gtactgtttc
     aattaaaaaa ggtaattcaa atgaaattgt taaatgtaat taattttgtt ttcttgatgt
     ttgtttcatc atcttctttt gctcaagtaa ttgaaatgaa taattcgcct ctgcgcgatt
     tcgtgacttg gtattcaaag caaacaggtg aatctgttat tgtctcacct gatgttaaag
     gtacagtgac tgtatattcc tctgacgtta agcctgaaaa tttacgcaat ttctttatct
     ctgttttacg tgctaataat tttgatatgg ttggctcaat tccttccata attcagaaat
     ataacccaaa tagtcaggat tatattgatg aattgccatc atctgatatt caggaatatg
     atgataattc cgctccttct ggtggtttct ttgttccgca aaatgataat gttactcaaa
     catttaaaat taataacgtt cgcgcaaagg atttaataag ggttgtagaa ttgtttgtta
     aatctaatac atctaaatcc tcaaatgtat tatctgttga tggttctaac ttattagtag
     ttagcgcccc taaagatatt ttagataacc ttccgcaatt tctttctact gttgatttgc
     caactgacca gatattgatt gaaggattaa ttttcgaggt tcagcaaggt gatgctttag
     atttttcctt tgctgctggc tctcagcgcg gcactgttgc tggtggtgtt aatactgacc
     gtctaacctc tgttttatct tctgcgggtg gttcgttcgg tatttttaac ggcgatgttt
     tagggctatc agttcgcgca ttaaagacta atagccattc aaaaatattg tctgtgcctc
     gtattcttac gctttcaggt cagaagggtt ctatttctgt tggccagaat gtccctttta
     ttactggtcg tgtaactggt gaatctgcca atgtaaataa tccatttcag acggttgagc
     gtcaaaatgt tggtatttct atgagtgttt ttcccgttgc aatggctggc ggtaatattg
     ttttagatat aaccagtaag gccgatagtt tgagttcttc tactcaggca agtgatgtta
     ttactaatca aagaagtatt gcgacaacgg ttaatttgcg tgatggtcag actcttttgc
     tcggtggcct cactgattac aaaaacactt ctcaagattc tggtgtgccg ttcctgtcta
     aaatcccttt aatcggcctc ctgtttagct cccgttctga ttctaacgag gaaagcacgt
     tgtacgtgct cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     ttcgctttct tcccttcctt tctcgccacg ttctccggct ttccccgtca agctctaaat
     cgggggatcc ctttagggtt ccgatttagt gctttacggc acctcgacct ccaaaaactt
     gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaca
     actaactcgg cctattcttt tgatttataa ggatttttgt cattttctgc ttactggtta
     aaaaataagc tgatttaaca aatatttaac gcgaaattta acaaaacatt aacgtttaca
     atttaaatat ttgcttatac aatcatcctg tttttggggc ttttctgatt atcaaccggg
     gtacatatga ttgacatgct agttttacga ttaccgttca tcgattctct tgtttgctcc
     agactttcag gtaatgacct gatagccttt gtagacctct caaaaatagc taccctctcc
     ggcatgaatt tatcagctag aacggttgaa tatcatattg acggtgattt gactgtctcc
     ggcctttctc acccgtttga atctttgcct actcattact ccggcattgc atttaaaata
     tatgagggtt ctaaaaattt ttatccctgc gttgaaatta aggcttcacc agcaaaagta
     ttacagggtc ataatgtttt tggtacaacc gatttagctt tatgctctga ggctttattg
     cttaattttg ctaactctct gccttgcttg tacgatttat tggatgtt
//