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ID   FDTET      preliminary; DNA; SYN; 9183 BP.
XX
AC   ATCC37000;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector fd-tet - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   fd-tet from phage fd & pRT44, Tn10 tet gene
RA   Zacher A.N., Stock C.A., Golden J.W., Smith G.P.;
RT   "A new filamentous phage cloning vector: fd-tet";
RL   Gene 9:127-140(1980).
XX
RN   [2]
RC   fdCAT1 from fd-tet
RA   McCafferty J., Griffiths A.D., Winter G., Chiswell D.J.;
RT   "Phage antibodies: filamentous phage displaying antibody variable
RT   domains";
RL   Nature 348:552-554(1990).
XX
RN   [3]
RC   pRT44 from ColE1 & lambda::Tn10(1), Tn10 tet gene
RC   pRT61 from pRT44
RA   Jorgensen R., Berg D.E., Allet B., Reznikoff W.S.;
RT   "Restriction enzyme cleavage map of Tn10, a transposon which
RT   encodes tetracycline resistance";
RL   J. Bacteriol. 137:681-685(1979).
XX
RN   [4]
RC   fd11 from phage fd & pKB252
RC   fd101 from phage fd & pACYC177
RC   fd102 from phage fd & pACYC177
RC   fd103 from phage fd & pACYC177 & pACYC184
RC   fd104 from phage fd & pACYC177
RC   fd105 from phage fd & pACYC184
RC   fd106 from phage fd & pACYC177 & pACYC184
RC   fd107 from phage fd & pBR322
RC   fd109 from phage fd & pBR322
RC   from fd106 & pGVO421
RA   Herrmann R., Neugebauer K., Pirkl E., Zentgraf H., Schaller H.;
RT   "Conversion of bacteriophage fd into an efficient single-stranded
RT   DNA vector system";
RL   Mol. Gen. Genet. 177:231-242(1980).
XX
RN   [5]
RC   fdY1 from fd106 & YEp6, HIS3 gene
RC   fdY5 from fd106 & yeast 2 micron
RC   fdY7 from fdY1 & yeast 2 micron
RC   fdY9 from fdY1 & yeast 2 micron
RA   Singh H., Bieker J.J., Dumas L.B.;
RT   "Genetic transformation of Saccharomyces cerevisiae with
RT   single-stranded circular DNA vectors";
RL   Gene 20:441-449(1982).
XX
RN   [6]
RC   lambda::Tn10(1) from lambda & Tn10
RC   lambda::Tn10(2) from lambda & Tn10
RA   Jorgensen R.A.;
RT   ;
RL   Thesis [Univ. of Wisconsin] 0:0-0(1978).
XX
RN   [7]
RC   pGVO421 from pBR322 & Ti plasmid
RC   pGVO415 from RSF2124 & Ti plasmid
RA   Depicker A., De Wilde M., De Vos G., De Vos R., Van Montagu M.,
RA   Schell J.;
RT   "Molecular cloning of overlapping segments of the nopaline
RT   Ti-plasmid pTiC58 as a means to restriction endonuclease mapping";
RL   Plasmid 3:193-211(1980).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Has been used as the starting material to construct a vector
CC   expressing immunoglobulin variable region-gene III fusion proteins
CC   which can be screened by antigen with the intact bacteriophage
CC   (phage antibodies). [3]
CC   Distributed as infected E.coli JM101 (ATCC 33876). (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (fd-tet)
CC   CM (yes)
CC   NA (filamentous ss-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (E.coli JM101 [ATCC 33876])(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (phage fd)(Tn10)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   2. Tn10, tet gene
FT                   -> lambda::Tn10(1) 2800bp
FT                   1. lambda::Tn10(1) 2800bp, Tn10 tet gene
FT                   insertion
FT                   2. ColE1 6646bp
FT                   -> pRT44 9400bp
FT                   1. phage fd BamHI 6408bp 6273..6273, near ori
FT                   2. pRT44 BglII-BglII 2775bp,
FT                   \ Tn10 tet/#J01830 628..3402
FT                   -> fd-tet 9183bp"
FT   -               1..6272
FT                   /note="phage fd 1..6272 6272bp
FT                   BamHI = G^GATCC
FT                   BglII = A^GATCT"
FT   -               6273..9047
FT                   /note="Tn10 628..3402 2775bp
FT                   BglII = A^GATCT
FT                   BamHI = G^GATCC"
FT   -               9048..9183
FT                   /note="phage fd 6273..6408 136bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage fd"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 9183 BP; 2307 A; 1819 C; 1900 G; 3157 T; 0 other;
     aatagtggac tcttgttcca aactggaaca acactcacaa ctaactcggc ctattctttt
     gatttataag gatttttgtc attttctgct tactggttaa aaaataagct gatttaacaa
     atatttaacg cgaaatttaa caaaacatta acgtttacaa tttaaatatt tgcttataca
     atcatcctgt ttttggggct tttctgatta tcaaccgggg tacatatgat tgacatgcta
     gttttacgat taccgttcat cgattctctt gtttgctcca gactttcagg taatgacctg
     atagcctttg tagacctctc aaaaatagct accctctccg gcatgaattt atcagctaga
     acggttgaat atcatattga cggtgatttg actgtctccg gcctttctca cccgtttgaa
     tctttgccta ctcattactc cggcattgca tttaaaatat atgagggttc taaaaatttt
     tatccctgcg ttgaaattaa ggcttcacca gcaaaagtat tacagggtca taatgttttt
     ggtacaaccg atttagcttt atgctctgag gctttattgc ttaattttgc taactctctg
     ccttgcttgt acgatttatt ggatgttaac gctactacca ttagtagaat tgatgccacc
     ttttcagctc gcgccccaaa tgaaaatata gctaaacagg ttattgacca tttgcgaaat
     gtatctaatg gtcaaactaa atctactcgt tcgcagaatt gggaatcaac tgttacatgg
     aatgaaactt ccagacaccg tactttagtt gcatatttaa aacatgttga actacagcac
     cagattcagc aattaagctc taagccatcc gcaaaaatga cctcttatca aaaggagcaa
     ttaaaggtac tgtctaatcc tgacctgttg gaatttgctt ccggtctggt tcgctttgag
     gctcgaattg aaacgcgata tttgaagtct ttcgggcttc ctcttaatct ttttgatgca
     attcgctttg cttctgacta taatagacag ggtaaagacc tgatttttga tttatggtca
     ttctcgtttt ctgaactgtt taaagcattt gagggggatt caatgaatat ttatgacgat
     tccgcagtat tggacgctat ccagtctaaa cattttacaa ttaccccctc tggcaaaact
     tcctttgcaa aagcctctcg ctattttggt ttctatcgtc gtctggttaa tgagggttat
     gatagtgttg ctcttaccat gcctcgtaat tccttttggc gttatgtatc tgcattagtt
     gagtgtggta ttcctaaatc tcaattgatg aatctttcca cctgtaataa tgttgttccg
     ttagttcgtt ttattaacgt agatttttcc tcccaacgtc ctgactggta taatgagcca
     gttcttaaaa tcgcataagg taattcaaaa tgattaaagt tgaaattaaa ccgtctcaag
     cgcaatttac tacccgttct ggtgtttctc gtcagggcaa gccttattca ctgaatgagc
     agctttgtta cgttgatttg ggtaatgaat atccggtgct tgtcaagatt actctcgacg
     aaggtcagcc agcgtatgcg cctggtctgt acaccgtgca tctgtcctcg ttcaaagttg
     gtcagttcgg ttctcttatg attgaccgtc tgcgcctcgt tccggctaag taacatggag
     caggtcgcgg atttcgacac aatttatcag gcgatgatac aaatctccgt tgtactttgt
     ttcgcgcttg gtataatcgc tgggggtcaa agatgagtgt tttagtgtat tctttcgcct
     ctttcgtttt aggttggtgc cttcgtagtg gcattacgta ttttacccgt ttaatggaaa
     cttcctcatg aaaaagtctt tagtcctcaa agcctccgta gccgttgcta ccctcgttcc
     gatgctgtct ttcgctgctg agggtgacga tcccgcaaaa gcggcctttg actccctgca
     agcctcagcg accgaatata tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc
     aactatcggt atcaagctgt ttaagaaatt cacctcgaaa gcaagctgat aaaccgatac
     aattaaaggc tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta
     ttcgcaattc ctttagttgt tcctttctat tctcactccg ctgaaactgt tgaaagttgt
     ttagcaaaac ctcatacaga aaattcattt actaacgtct ggaaagacga caaaacttta
     gatcgttacg ctaactatga gggctgtctg tggaatgcta caggcgttgt ggtttgtact
     ggtgacgaaa ctcagtgtta cggtacatgg gttcctattg ggcttgctat ccctgaaaat
     gagggtggtg gctctgaggg tggcggttct gagggtggcg gttctgaggg tggcggtact
     aaacctcctg agtacggtga tacacctatt ccgggctata cttatatcaa ccctctcgac
     ggcacttatc cgcctggtac tgagcaaaac cccgctaatc ctaatccttc tcttgaggag
     tctcagcctc ttaatacttt catgtttcag aataataggt tccgaaatag gcagggtgca
     ttaactgttt atacgggcac tgttactcaa ggcactgacc ccgttaaaac ttattaccag
     tacactcctg tatcatcaaa agccatgtat gacgcttact ggaacggtaa attcagagac
     tgcgctttcc attctggctt taatgaggat ccattcgttt gtgaatatca aggccaatcg
     tctgacctgc ctcaacctcc tgtcaatgct ggcggcggct ctggtggtgg ttctggtggc
     ggctctgagg gtggcggctc tgagggtggc ggttctgagg gtggcggctc tgagggtggc
     ggttccggtg gcggctccgg ttccggtgat tttgattatg aaaaaatggc aaacgctaat
     aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc taaaggcaaa
     cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg tgacgtttcc
     ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc ccaaatggct
     caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata tttaccttct
     ttgcctcagt cggttgaatg tcgcccttat gtctttggcg ctggtaaacc atatgaattt
     tctattgatt gtgacaaaat aaacttattc cgtggtgtct ttgcgtttct tttatatgtt
     gccaccttta tgtatgtatt ttcgacgttt gctaacatac tgcgtaataa ggagtcttaa
     tcatgccagt tcttttgggt attccgttat tattgcgttt cctcggtttc cttctggtaa
     ctttgttcgg ctatctgctt actttcctta aaaagggctt cggtaagata gctattgcta
     tttcattgtt tcttgctctt attattgggc ttaactcaat tcttgtgggt tatctctctg
     atattagcgc acaattaccc tctgattttg ttcagggcgt tcagttaatt ctcccgtcta
     atgcgcttcc ctgtttttat gttattctct ctgtaaaggc tgctattttc atttttgacg
     ttaaacaaaa aatcgtttct tatttggatt gggataaata aatatggctg tttattttgt
     aactggcaaa ttaggctctg gaaagacgct cgttagcgtt ggtaagattc aggataaaat
     tgtagctggg tgcaaaatag caactaatct tgatttaagg cttcaaaacc tcccgcaagt
     cgggaggttc gctaaaacgc ctcgcgttct tagaataccg gataagcctt ctatttctga
     tttgcttgct attggtcgtg gtaatgattc ctacgacgaa aataaaaacg gtttgcttgt
     tcttgatgaa tgcggtactt ggtttaatac ccgttcatgg aatgacaagg aaagacagcc
     gattattgat tggtttcttc atgctcgtaa attgggatgg gatattattt ttcttgttca
     ggatttatct attgttgata aacaggcgcg ttctgcatta gctgaacacg ttgtttattg
     tcgccgtctg gacagaatta ctttaccctt tgtcggcact ttatattctc ttgttactgg
     ctcaaaaatg cctctgccta aattacatgt tggtgttgtt aaatatggtg attctcaatt
     aagccctact gttgagcgtt ggctttatac tggtaagaat ttatataacg catatgacac
     taaacaggct ttttccagta attatgattc aggtgtttat tcatatttaa ccccttattt
     atcacacggt cggtatttca aaccattaaa tttaggtcag aagatgaaat taactaaaat
     atatttgaaa aagttttctc gcgttctttg tcttgcgata ggatttgcat cagcatttac
     atatagttat ataacccaac ctaagccgga ggttaaaaag gtagtctctc agacctatga
     ttttgataaa ttcactattg actcttctca gcgtcttaat ctaagctatc gctatgtttt
     caaggattct aagggaaaat taattaatag cgacgattta cagaagcaag gttattccat
     cacatatatt gatttatgta ctgtttcaat taaaaaaggt aattcaaatg aaattgttaa
     atgtaattaa ttttgttttc ttgatgtttg tttcatcatc ttcttttgct caagtaattg
     aaatgaataa ttcgcctctg cgcgatttcg tgacttggta ttcaaagcaa acaggtgaat
     ctgttattgt ctcacctgat gttaaaggta cagtgactgt atattcctct gacgttaagc
     ctgaaaattt acgcaatttc tttatctctg ttttacgtgc taataatttt gatatggttg
     gctcaattcc ttccataatt cagaaatata acccaaatag tcaggattat attgatgaat
     tgccatcatc tgatattcag gaatatgatg ataattccgc tccttctggt ggtttctttg
     ttccgcaaaa tgataatgtt actcaaacat ttaaaattaa taacgttcgc gcaaaggatt
     taataagggt tgtagaattg tttgttaaat ctaatacatc taaatcctca aatgtattat
     ctgttgatgg ttctaactta ttagtagtta gcgcccctaa agatatttta gataaccttc
     cgcaatttct ttctactgtt gatttgccaa ctgaccagat attgattgaa ggattaattt
     tcgaggttca gcaaggtgat gctttagatt tttcctttgc tgctggctct cagcgcggca
     ctgttgctgg tggtgttaat actgaccgtc taacctctgt tttatcttct gcgggtggtt
     cgttcggtat ttttaacggc gatgttttag ggctatcagt tcgcgcatta aagactaata
     gccattcaaa aatattgtct gtgcctcgta ttcttacgct ttcaggtcag aagggttcta
     tttctgttgg ccagaatgtc ccttttatta ctggtcgtgt aactggtgaa tctgccaatg
     taaataatcc atttcagacg gttgagcgtc aaaatgttgg tatttctatg agtgtttttc
     ccgttgcaat ggctggcggt aatattgttt tagatataac cagtaaggcc gatagtttga
     gttcttctac tcaggcaagt gatgttatta ctaatcaaag aagtattgcg acaacggtta
     atttgcgtga tggtcagact cttttgctcg gtggcctcac tgattacaaa aacacttctc
     aagattctgg tgtgccgttc ctgtctaaaa tccctttaat cggcctcctg tttagctccc
     gttctgattc taacgaggaa agcacgttgt acgtgctcgt caaagcaacc atagtacgcg
     ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     tccggctttc cccgtcaagc tctaaatcgg gggatctatg attccctttg tcaacagcaa
     tggatcactg aaaatggttc aatgatcaca ttaagtggta ttcaatattt tcatgaaatg
     ggaattgacg ttccttccaa acattcacgt aaaatctgtt gtgcgtgttt agattggagt
     gaacgccgtt tccatttagg tgggtacgtt ggagccgcat tattttcgct ttatgaatct
     aaagggtggt taactcgaca tcttggttac cgtgaagtta ccatcacgga aaaaggttat
     gctgctttta agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt
     caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc
     gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat
     gctcttgatc ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata
     atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt
     catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta
     gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt
     ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca
     aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg
     cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt
     taatcacttt acttttatct aatctagaca tcattaattc ctaatttttg ttgacactct
     atcattgata gagttatttt accactccct atcagtgata gagaaaagtg aaatgaatag
     ttcgacaaag atcgcattgg taattacgtt actcgatgcc atggggattg gccttatcat
     gccagtcttg ccaacgttat tacgtgaatt tattgcttcg gaagatatcg ctaaccactt
     tggcgtattg cttgcacttt atgcgttaat gcaggttatc tttgctcctt ggcttggaaa
     aatgtctgac cgatttggtc ggcgcccagt gctgttgttg tcattaatag gcgcatcgct
     ggattactta ttgctggctt tttcaagtgc gctttggatg ctgtatttag gccgtttgct
     ttcagggatc acaggagcta ctggggctgt cgcggcatcg gtcattgccg ataccacctc
     agcttctcaa cgcgtgaagt ggttcggttg gttaggggca agttttgggc ttggtttaat
     agcggggcct attattggtg gttttgcagg agagatttca ccgcatagtc ccttttttat
     cgctgcgttg ctaaatattg tcactttcct tgtggttatg ttttggttcc gtgaaaccaa
     aaatacacgt gataatacag ataccgaagt aggggttgag acgcaatcga attcggtata
     catcacttta tttaaaacga tgcccatttt gttgattatt tatttttcag cgcaattgat
     aggccaaatt cccgcaacgg tgtgggtgct atttaccgaa aatcgttttg gatggaatag
     catgatggtt ggcttttcat tagcgggtct tggtctttta cactcagtat tccaagcctt
     tgtggcagga agaatagcca ctaaatgggg cgaaaaaacg gcagtactgc tcgaatttat
     tgcagatagt agtgcatttg cctttttagc gtttatatct gaaggttggt tagatttccc
     tgttttaatt ttattggctg gtggtgggat cgctttacct gcattacagg gagtgatgtc
     tatccaaaca aagagtcatg agcaaggtgc tttacaggga ttattggtga gccttaccaa
     tgcaaccggt gttattggcc cattactgtt tactgttatt tataatcatt cactaccaat
     ttgggatggc tggatttgga ttattggttt agcgttttac tgtattatta tcctgctatc
     gatgaccttc atgttaaccc ctcaagctca ggggagtaaa caggagacaa gtgcttagtt
     atttcgtcac caaatgatgt tattccgcga aatataatga ccctcttgat aacccaagag
     ggcatttttt acgataaaga agatttagct tcaaataaaa cctatctatt ttatttatct
     ttcaagctca ataaaaagcc gcggtaaata gcaataaatt ggcctttttt atcggcaagc
     tcttttaggt ttttcgcatg tattgcgata tgcataaacc agccattgag taagttttta
     agcacatcac tatcataagc tttaagttgg ttctcttgga tcaatttgct gacaatggcg
     tttaccttac cagtaatgta ttcaaggcta attttttcaa gttcattcca accaatgata
     ggcatcactt cttggatagg gataaggttt ttattattat caataatata atcaagataa
     tgttcaaata tactttctaa ggcagaccaa ccatttgtta aatcagtttt tgttgtgatg
     taggcatcaa tcataattaa ttgctgctta taacaggcac tgagtaattg ttttttattt
     ttaaagtgat gataaaaggc acctttggtc accaacgctt ttcccgagat ccctttaggg
     ttccgattta gtgctttacg gcacctcgac ctccaaaaac ttgatttggg tgatggttca
     cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     ttt
//