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ID   FKN16      preliminary; DNA; SYN; 8676 BP.
XX
AC   ATCC37002;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector fd fKN 16 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   fKN16 from fd-tet
RC   [fBH16 from fKN16 & linker]
RA   Nelson F.K., Friedman S.M., Smith G.P.;
RT   "Filamentous phage DNA cloning vectors: a noninfective mutant with
RT   a nonpolar deletion in gene III";
RL   Virology 108:338-350(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Provided as lyophilized viral DNA. When reconstituted with 50 ul of
CC   sterile distilled water, the DNA concentration is about 10x10(11)
CC   ssDNA molecules/ml in 1% SDS, 10 mM Tris pH8, 10 mM HCl, 1 mM EDTA.
CC   (ATCC staff)
CC   This vector contains a deletion in the phage gene III. It exhibits a
CC   higher level of biological containment than fd-tet (ATCC 37000).
CC   (ATCC staff)
CC   fd bacteriophage vector. (ATCC staff)
CC   Medium is 1592 SM buffer.
CC   NM (fKN 16)
CC   CM (yes)
CC   NA (filamentous ss-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteriophage fd)(E.coli K802)(E.coli C600)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (fd-tet)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. fd-tet remove Sau3A/MboI-BamHI 507bp
FT                   \ 2341..2848, gene III/9100bp
FT                   -> fKN16 9100bp"
FT   -               1..2340
FT                   /note="fd-tet 1..2340 2340bp
FT                   MboI = Sau3AI = ^GATC
FT                   BamHI =        G^GATCC"
FT   -               2341..8676
FT                   /note="fd-tet 2848..9183 6336bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage fd"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 8676 BP; 2189 A; 1704 C; 1773 G; 3010 T; 0 other;
     aatagtggac tcttgttcca aactggaaca acactcacaa ctaactcggc ctattctttt
     gatttataag gatttttgtc attttctgct tactggttaa aaaataagct gatttaacaa
     atatttaacg cgaaatttaa caaaacatta acgtttacaa tttaaatatt tgcttataca
     atcatcctgt ttttggggct tttctgatta tcaaccgggg tacatatgat tgacatgcta
     gttttacgat taccgttcat cgattctctt gtttgctcca gactttcagg taatgacctg
     atagcctttg tagacctctc aaaaatagct accctctccg gcatgaattt atcagctaga
     acggttgaat atcatattga cggtgatttg actgtctccg gcctttctca cccgtttgaa
     tctttgccta ctcattactc cggcattgca tttaaaatat atgagggttc taaaaatttt
     tatccctgcg ttgaaattaa ggcttcacca gcaaaagtat tacagggtca taatgttttt
     ggtacaaccg atttagcttt atgctctgag gctttattgc ttaattttgc taactctctg
     ccttgcttgt acgatttatt ggatgttaac gctactacca ttagtagaat tgatgccacc
     ttttcagctc gcgccccaaa tgaaaatata gctaaacagg ttattgacca tttgcgaaat
     gtatctaatg gtcaaactaa atctactcgt tcgcagaatt gggaatcaac tgttacatgg
     aatgaaactt ccagacaccg tactttagtt gcatatttaa aacatgttga actacagcac
     cagattcagc aattaagctc taagccatcc gcaaaaatga cctcttatca aaaggagcaa
     ttaaaggtac tgtctaatcc tgacctgttg gaatttgctt ccggtctggt tcgctttgag
     gctcgaattg aaacgcgata tttgaagtct ttcgggcttc ctcttaatct ttttgatgca
     attcgctttg cttctgacta taatagacag ggtaaagacc tgatttttga tttatggtca
     ttctcgtttt ctgaactgtt taaagcattt gagggggatt caatgaatat ttatgacgat
     tccgcagtat tggacgctat ccagtctaaa cattttacaa ttaccccctc tggcaaaact
     tcctttgcaa aagcctctcg ctattttggt ttctatcgtc gtctggttaa tgagggttat
     gatagtgttg ctcttaccat gcctcgtaat tccttttggc gttatgtatc tgcattagtt
     gagtgtggta ttcctaaatc tcaattgatg aatctttcca cctgtaataa tgttgttccg
     ttagttcgtt ttattaacgt agatttttcc tcccaacgtc ctgactggta taatgagcca
     gttcttaaaa tcgcataagg taattcaaaa tgattaaagt tgaaattaaa ccgtctcaag
     cgcaatttac tacccgttct ggtgtttctc gtcagggcaa gccttattca ctgaatgagc
     agctttgtta cgttgatttg ggtaatgaat atccggtgct tgtcaagatt actctcgacg
     aaggtcagcc agcgtatgcg cctggtctgt acaccgtgca tctgtcctcg ttcaaagttg
     gtcagttcgg ttctcttatg attgaccgtc tgcgcctcgt tccggctaag taacatggag
     caggtcgcgg atttcgacac aatttatcag gcgatgatac aaatctccgt tgtactttgt
     ttcgcgcttg gtataatcgc tgggggtcaa agatgagtgt tttagtgtat tctttcgcct
     ctttcgtttt aggttggtgc cttcgtagtg gcattacgta ttttacccgt ttaatggaaa
     cttcctcatg aaaaagtctt tagtcctcaa agcctccgta gccgttgcta ccctcgttcc
     gatgctgtct ttcgctgctg agggtgacga tcccgcaaaa gcggcctttg actccctgca
     agcctcagcg accgaatata tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc
     aactatcggt atcaagctgt ttaagaaatt cacctcgaaa gcaagctgat aaaccgatac
     aattaaaggc tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta
     ttcgcaattc ctttagttgt tcctttctat tctcactccg ctgaaactgt tgaaagttgt
     ttagcaaaac ctcatacaga aaattcattt actaacgtct ggaaagacga caaaacttta
     gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt
     ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt
     gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     ttaatgaata atttccgtca atatttacct tctttgcctc agtcggttga atgtcgccct
     tatgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttcgacg
     tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttactttcc
     ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     ggcttaactc aattcttgtg ggttatctct ctgatattag cgcacaatta ccctctgatt
     ttgttcaggg cgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     attgggataa ataaatatgg ctgtttattt tgtaactggc aaattaggct ctggaaagac
     gctcgttagc gttggtaaga ttcaggataa aattgtagct gggtgcaaaa tagcaactaa
     tcttgattta aggcttcaaa acctcccgca agtcgggagg ttcgctaaaa cgcctcgcgt
     tcttagaata ccggataagc cttctatttc tgatttgctt gctattggtc gtggtaatga
     ttcctacgac gaaaataaaa acggtttgct tgttcttgat gaatgcggta cttggtttaa
     tacccgttca tggaatgaca aggaaagaca gccgattatt gattggtttc ttcatgctcg
     taaattggga tgggatatta tttttcttgt tcaggattta tctattgttg ataaacaggc
     gcgttctgca ttagctgaac acgttgttta ttgtcgccgt ctggacagaa ttactttacc
     ctttgtcggc actttatatt ctcttgttac tggctcaaaa atgcctctgc ctaaattaca
     tgttggtgtt gttaaatatg gtgattctca attaagccct actgttgagc gttggcttta
     tactggtaag aatttatata acgcatatga cactaaacag gctttttcca gtaattatga
     ttcaggtgtt tattcatatt taacccctta tttatcacac ggtcggtatt tcaaaccatt
     aaatttaggt cagaagatga aattaactaa aatatatttg aaaaagtttt ctcgcgttct
     ttgtcttgcg ataggatttg catcagcatt tacatatagt tatataaccc aacctaagcc
     ggaggttaaa aaggtagtct ctcagaccta tgattttgat aaattcacta ttgactcttc
     tcagcgtctt aatctaagct atcgctatgt tttcaaggat tctaagggaa aattaattaa
     tagcgacgat ttacagaagc aaggttattc catcacatat attgatttat gtactgtttc
     aattaaaaaa ggtaattcaa atgaaattgt taaatgtaat taattttgtt ttcttgatgt
     ttgtttcatc atcttctttt gctcaagtaa ttgaaatgaa taattcgcct ctgcgcgatt
     tcgtgacttg gtattcaaag caaacaggtg aatctgttat tgtctcacct gatgttaaag
     gtacagtgac tgtatattcc tctgacgtta agcctgaaaa tttacgcaat ttctttatct
     ctgttttacg tgctaataat tttgatatgg ttggctcaat tccttccata attcagaaat
     ataacccaaa tagtcaggat tatattgatg aattgccatc atctgatatt caggaatatg
     atgataattc cgctccttct ggtggtttct ttgttccgca aaatgataat gttactcaaa
     catttaaaat taataacgtt cgcgcaaagg atttaataag ggttgtagaa ttgtttgtta
     aatctaatac atctaaatcc tcaaatgtat tatctgttga tggttctaac ttattagtag
     ttagcgcccc taaagatatt ttagataacc ttccgcaatt tctttctact gttgatttgc
     caactgacca gatattgatt gaaggattaa ttttcgaggt tcagcaaggt gatgctttag
     atttttcctt tgctgctggc tctcagcgcg gcactgttgc tggtggtgtt aatactgacc
     gtctaacctc tgttttatct tctgcgggtg gttcgttcgg tatttttaac ggcgatgttt
     tagggctatc agttcgcgca ttaaagacta atagccattc aaaaatattg tctgtgcctc
     gtattcttac gctttcaggt cagaagggtt ctatttctgt tggccagaat gtccctttta
     ttactggtcg tgtaactggt gaatctgcca atgtaaataa tccatttcag acggttgagc
     gtcaaaatgt tggtatttct atgagtgttt ttcccgttgc aatggctggc ggtaatattg
     ttttagatat aaccagtaag gccgatagtt tgagttcttc tactcaggca agtgatgtta
     ttactaatca aagaagtatt gcgacaacgg ttaatttgcg tgatggtcag actcttttgc
     tcggtggcct cactgattac aaaaacactt ctcaagattc tggtgtgccg ttcctgtcta
     aaatcccttt aatcggcctc ctgtttagct cccgttctga ttctaacgag gaaagcacgt
     tgtacgtgct cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     ttcgctttct tcccttcctt tctcgccacg ttctccggct ttccccgtca agctctaaat
     cgggggatct atgattccct ttgtcaacag caatggatca ctgaaaatgg ttcaatgatc
     acattaagtg gtattcaata ttttcatgaa atgggaattg acgttccttc caaacattca
     cgtaaaatct gttgtgcgtg tttagattgg agtgaacgcc gtttccattt aggtgggtac
     gttggagccg cattattttc gctttatgaa tctaaagggt ggttaactcg acatcttggt
     taccgtgaag ttaccatcac ggaaaaaggt tatgctgctt ttaagaccca ctttcacatt
     taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg
     caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag
     taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta
     aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt
     ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac
     ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt
     tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc
     tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat
     acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga
     ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag
     acatcattaa ttcctaattt ttgttgacac tctatcattg atagagttat tttaccactc
     cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat tggtaattac
     gttactcgat gccatgggga ttggccttat catgccagtc ttgccaacgt tattacgtga
     atttattgct tcggaagata tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt
     aatgcaggtt atctttgctc cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc
     agtgctgttg ttgtcattaa taggcgcatc gctggattac ttattgctgg ctttttcaag
     tgcgctttgg atgctgtatt taggccgttt gctttcaggg atcacaggag ctactggggc
     tgtcgcggca tcggtcattg ccgataccac ctcagcttct caacgcgtga agtggttcgg
     ttggttaggg gcaagttttg ggcttggttt aatagcgggg cctattattg gtggttttgc
     aggagagatt tcaccgcata gtcccttttt tatcgctgcg ttgctaaata ttgtcacttt
     ccttgtggtt atgttttggt tccgtgaaac caaaaataca cgtgataata cagataccga
     agtaggggtt gagacgcaat cgaattcggt atacatcact ttatttaaaa cgatgcccat
     tttgttgatt atttattttt cagcgcaatt gataggccaa attcccgcaa cggtgtgggt
     gctatttacc gaaaatcgtt ttggatggaa tagcatgatg gttggctttt cattagcggg
     tcttggtctt ttacactcag tattccaagc ctttgtggca ggaagaatag ccactaaatg
     gggcgaaaaa acggcagtac tgctcgaatt tattgcagat agtagtgcat ttgccttttt
     agcgtttata tctgaaggtt ggttagattt ccctgtttta attttattgg ctggtggtgg
     gatcgcttta cctgcattac agggagtgat gtctatccaa acaaagagtc atgagcaagg
     tgctttacag ggattattgg tgagccttac caatgcaacc ggtgttattg gcccattact
     gtttactgtt atttataatc attcactacc aatttgggat ggctggattt ggattattgg
     tttagcgttt tactgtatta ttatcctgct atcgatgacc ttcatgttaa cccctcaagc
     tcaggggagt aaacaggaga caagtgctta gttatttcgt caccaaatga tgttattccg
     cgaaatataa tgaccctctt gataacccaa gagggcattt tttacgataa agaagattta
     gcttcaaata aaacctatct attttattta tctttcaagc tcaataaaaa gccgcggtaa
     atagcaataa attggccttt tttatcggca agctctttta ggtttttcgc atgtattgcg
     atatgcataa accagccatt gagtaagttt ttaagcacat cactatcata agctttaagt
     tggttctctt ggatcaattt gctgacaatg gcgtttacct taccagtaat gtattcaagg
     ctaatttttt caagttcatt ccaaccaatg ataggcatca cttcttggat agggataagg
     tttttattat tatcaataat ataatcaaga taatgttcaa atatactttc taaggcagac
     caaccatttg ttaaatcagt ttttgttgtg atgtaggcat caatcataat taattgctgc
     ttataacagg cactgagtaa ttgtttttta tttttaaagt gatgataaaa ggcacctttg
     gtcaccaacg cttttcccga gatcccttta gggttccgat ttagtgcttt acggcacctc
     gacctccaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     gtttttcgcc ctttgacgtt ggagtccacg ttcttt
//