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ID KSSV0CAT preliminary; circular DNA; SYN; 4841 BP.
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AC ATCC37608;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Vertebrate/E.coli plasmid vector KS+SV0CAT - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC KS+SV2CAT from pBluescript KS+ & pSV2cat
RC KS+SV0CAT from pBluescript KS+ & pSV2cat
RA Tsonis P.A., Manes T., Millan J.L., Goetinck P.F.;
RT "CAT constructs with convenient sites for cloning and generating
RT deletions";
RL Nucleic Acids Res. 16:7745-7745(1988).
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CC Created by Moore, July 1995, under contract with NCBI.
CC HindIII-ApaI fragment from pSV2cat contains internal BamHI and EcoRI
CC sites.
CC Mammalian shuttle expression vector for promoter cloning with activity
CC analysis by CAT (chloramphenicol acetyl transferase) assay. Use
CC KS+SV2CAT (ATCC 37609) as a control.
CC Restriction digests of the clone give the following sizes (kb):
CC SacI--4.4; Xba--4.4; BamHI--2.9, 1.6; SmaI--4.4; PstI--4.4;
CC EcoRI--3.9, 0.32; HindIII--4.4. (ATCC staff)
CC Constructed by inserting the HindIII-ApaI fragment of pSV2cat in
CC HindIII-ApaI digested Bluescript KS+. Contains no SV40 promoter.
CC (personal communication)
CC Medium is 1227 LB plus ampicillin.
CC NM (KS+SV0CAT)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(vertebrate cells)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBluescript KS+)(pSV2-cat)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBluescript KS+ remove HindIII-ApaI 34bp
FT \ 720..754, 2930bp [2929bp]
FT 2. pSV2-cat ApaI-HindIII 1903bp 3099..5002, cat gene
FT -> KS+SV0CAT 4833bp [EcoRI in cat]"
FT - 1..719
FT /note="pBluescript KS+ 1..719 719bp
FT HindIII = A^AGCT T
FT \ T TCGA^A"
FT - 720..2630
FT /note="pSV2-cat 3095..5003..2 1911bp complement
FT ApaI = G GGCC^C
FT \ C^CCGG G"
FT - 2631..4841
FT /note="pBluescript KS+ 754..2964 2211bp"
FT misc_binding 0..0
FT /note="MCS SacI-BstXI-SacII-EagI-NotI-XbaI-SpeI-
FT BamHI-SmaI-PstI-EcoRI-EcoRV-HindIII-ApaI-KpnI"
FT misc_binding 0..0
FT /note="SIT unique SacI-BstXI-SacII-EagI-NotI-XbaI-
FT SpeI-SmaI-PstI-EcoRV"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT CDS 0..0
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (cat); chloramphenicol resistance gene (cmr/cml);
FT reporter gene"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 4841 BP; 1308 A; 1110 C; 1129 G; 1294 T; 0 other;
ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac
aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
gccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
tatcatgtct ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac
ttgagaggac attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag
gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat
tttaaaatat ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc
acaaatgtca acagcagaaa catacaagct gtcagctttg cacaagggcc cggtacccag
cttttgttcc ctttagtgag ggttaattcc gagcttggcg taatcatggt catagctgtt
tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//