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ID   KSSV0CAT   preliminary; circular DNA; SYN; 4841 BP.
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AC   ATCC37608;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector KS+SV0CAT - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   KS+SV2CAT from pBluescript KS+ & pSV2cat
RC   KS+SV0CAT from pBluescript KS+ & pSV2cat
RA   Tsonis P.A., Manes T., Millan J.L., Goetinck P.F.;
RT   "CAT constructs with convenient sites for cloning and generating
RT   deletions";
RL   Nucleic Acids Res. 16:7745-7745(1988).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   HindIII-ApaI fragment from pSV2cat contains internal BamHI and EcoRI
CC   sites.
CC   Mammalian shuttle expression vector for promoter cloning with activity
CC   analysis by CAT (chloramphenicol acetyl transferase) assay.  Use
CC   KS+SV2CAT (ATCC 37609) as a control.
CC   Restriction digests of the clone give the following sizes (kb):
CC   SacI--4.4; Xba--4.4; BamHI--2.9, 1.6; SmaI--4.4; PstI--4.4;
CC   EcoRI--3.9, 0.32; HindIII--4.4. (ATCC staff)
CC   Constructed by inserting the HindIII-ApaI fragment of pSV2cat in
CC   HindIII-ApaI digested Bluescript KS+. Contains no SV40 promoter.
CC   (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (KS+SV0CAT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBluescript KS+)(pSV2-cat)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript KS+ remove HindIII-ApaI 34bp
FT                   \ 720..754, 2930bp [2929bp]
FT                   2. pSV2-cat ApaI-HindIII 1903bp 3099..5002, cat gene
FT                   -> KS+SV0CAT 4833bp [EcoRI in cat]"
FT   -               1..719
FT                   /note="pBluescript KS+ 1..719 719bp
FT                   HindIII = A^AGCT T
FT                   \         T TCGA^A"
FT   -               720..2630
FT                   /note="pSV2-cat 3095..5003..2 1911bp complement
FT                   ApaI = G GGCC^C
FT                   \      C^CCGG G"
FT   -               2631..4841
FT                   /note="pBluescript KS+ 754..2964 2211bp"
FT   misc_binding    0..0
FT                   /note="MCS SacI-BstXI-SacII-EagI-NotI-XbaI-SpeI-
FT                   BamHI-SmaI-PstI-EcoRI-EcoRV-HindIII-ApaI-KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-BstXI-SacII-EagI-NotI-XbaI-
FT                   SpeI-SmaI-PstI-EcoRV"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 4841 BP; 1308 A; 1110 C; 1129 G; 1294 T; 0 other;
     ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
     gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
     ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
     gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
     cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     gccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     tatcatgtct ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac
     ttgagaggac attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag
     gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat
     tttaaaatat ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc
     acaaatgtca acagcagaaa catacaagct gtcagctttg cacaagggcc cggtacccag
     cttttgttcc ctttagtgag ggttaattcc gagcttggcg taatcatggt catagctgtt
     tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//