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ID   KSSV2CAT   preliminary; circular DNA; SYN; 5373 BP.
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AC   ATCC37609;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector KS+SV2CAT - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   KS+SV2CAT from pBluescript KS+ & pSV2cat
RC   KS+SV0CAT from pBluescript KS+ & pSV2cat
RA   Tsonis P.A., Manes T., Millan J.L., Goetinck P.F.;
RT   "CAT constructs with convenient sites for cloning and generating
RT   deletions";
RL   Nucleic Acids Res. 16:7745-7745(1988).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Mammalian shuttle expression vector containing a CAT (chloramphenicol
CC   acetyl transferase) gene expressed under an SV40 promoter. To be used
CC   as a control for promoter activity in conjuction with the promoter
CC   cloning plasmid KS+SV0CAT (ATCC 37608).
CC   Additional polylinker sites are ApaI and KpnI.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--2.8, 2.1; SmaI--4.7; PstI--4.7; EcoRI--4.l, 0.72; ClaI--4.7.
CC   (ATCC staff)
CC   Constructed by inserting the AccI-ApaI fragment of pSV2cat into the
CC   EcoRV site of Bluescript KS+.  Ends were blunt. (personal
CC   communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (KS+SV2CAT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(vertebrate cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBluescript KS+)(pSV2-cat)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript KS+ EcoRV 2964bp 716..716
FT                   2. pSV2-cat ApaI-AccI 2409bp 3099..5003..505,
FT                   \ SV40 promoter/cat gene
FT                   blunt end:blunt end
FT                   -> KS+SV2CAT 5373bp"
FT   -               1..715
FT                   /note="pBluescript KS+ 1..715 715bp
FT                   EcoRV =   GAT^ATC
FT                   ApaI = G GGCC^C"
FT   -               716..3124
FT                   /note="pSV2-cat 3099..5003..504 2409bp
FT                   AccI =   GT^MKAC
FT                   EcoRV = GAT^ATC"
FT   -               3125..5373
FT                   /note="pBluescript KS+ 716..2964 2249bp"
FT   misc_binding    0..0
FT                   /note="MCS SacI-BstXI-SacII-EagI-NotI-XbaI-SpeI-
FT                   BamHI-SmaI-PstI-EcoRI-HindIII-ClaI-SalI-HincII-
FT                   AccI-XhoI-DraI"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-BstXI-SacII-EagI-NotI-XbaI-
FT                   SpeI-SmaI-PstI-HindIII-ClaI-
FT                   SalI-HincII-AccI-XhoI-DraI-ApaI-KpnI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO SV40"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
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SQ   Sequence 5373 BP; 1435 A; 1245 C; 1299 G; 1394 T; 0 other;
     ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
     gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
     ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatcttgt
     gcaaagctga cagcttgtat gtttctgctg ttgacatttg tgggctgttt accaacactt
     ctggaacaca gcagtggaag ggacttccca gatattttaa aattaccctt agaaagcggt
     ctgtgaaaaa cccctaccca atttcctttt tgttaagtga cctaattaac aggaggacac
     agagggtgga tgggcagcct atgattggaa tgtcctctca agtagaggag gttagggttt
     atgaggacac agaggagctt cctggggatc cagacatgat aagatacatt gatgagtttg
     gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca aatattcctt
     attaacccct ttacaaatta aaaagctaaa ggtacacaat ttttgagcat agttattaat
     agcagacact ctatgcctgt gtggagtaag aaaaaacagt atgttatgat tataactgtt
     atgcctactt ataaaggtta cagaatattt ttccataatt ttcttgtata gcagtgcagc
     tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg
     actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt
     ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc
     ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt
     tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
     acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
     tgtcacacca cagaagtaag gttccttcac aaagatccgg cgaatttctg ccattcatcc
     gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat aactgcctta
     aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac
     cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     ctccatttta gcttccttag ctcctgaaaa tctcgccaag ctttttgcaa aagcctaggc
     ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     tgctttgcat acttctgcct gctggggagc ctggttgctg actaattgag atgcatgctt
     tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
     cacagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     gtgtatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc agcttttgtt
     ccctttagtg agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     ttccgcgcac atttccccga aaagtgccac ctg
//