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ID   LAWRIST16  preliminary; circular DNA; SYN; 5194 BP.
XX
AC   L19898;
XX
DT   24-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector LAWRIST16 - complete.
XX
KW   cloning vector; T7; T3; cos; neo gene.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5194
RC   LAWRIST16 from lambda, cos
RA   Bodenteich A., Chissoe S.L., Wang Z., Roe B.A.;
RT   "Nucleotide sequence of three cosmid cloning vectors: svPHEP, pTCF,
RT   and LAWRIST16";
RL   Unpublished (1993).
XX
CC   The packaged version of the cosmid cloning vector constructed by
CC   P. de Jong, Biomedical Sciences Division, LLNL, Livermore, CA.
CC   NCBI gi: 310737
CC   NM (LAWRIST16)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   2. T7, promoter
FT                   3. T3, promoter
FT                   4. Tn5, neo gene
FT                   -> LAWRIST16 5194bp"
FT   misc_feature    236..247
FT                   /note="SIT bacteriophage lambda cos site"
FT   promoter        577..597
FT                   /note="PRO bacteriophage T7"
FT   promoter        620..641
FT                   /note="PRO bacteriophage T3"
FT   rep_origin      1790..2229
FT                   /note="ORI bacteriophage lambda"
FT   CDS             complement(3678..4682)
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo);
FT                   11 stops when translation attempted, frame 1, code 0"
XX
SQ   Sequence 5194 BP; 1200 A; 1332 C; 1333 G; 1329 T; 0 other;
     gatcgatccg ggcaacgttg ttgccattgc tgcagggggg gggggggggg ggttccattg
     ttcattccac ggacaaaaac agagaaagga aacgacagag gccaaaaagc tcgctttcag
     cacctgtcgt ttcctttctt ttcagagggt attttaaata aaaacattaa gttatgacga
     agaagaacgg aaacgcctta aaccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc
     gccgccccgt aacctgtcgg atcaccggaa aggacccgta aagtgataat gattatcatc
     tacatatcac aacgtgcgtg gaggccatca aaccacgtca aataatcaat tatgacgcag
     gtatcgtatt aattgatctg catcaactta acgtaaaaac aacttcagac aatacaaatc
     agcgacactg aatacggggc aacctcatgt cccccccccc cccccctgca ggcgcagaac
     tggtaggtat ggaagatctc tagaattcgg ccgaaacggc caaaaaaagc ccgctcatta
     agcgggctaa ttcgcctcga ggtggcttat cgaaattaat acgactcact atagggagac
     ccaagcttag gatcctatgt attcccttta gtgagggtaa ttctcgagga aatggtcagc
     gaaccaatca gcagggtcat cgctagaaac catccttagc gaaagctaag gatttttttt
     atctggccgc cccggccgaa ttctagccag atccccgctg atttatgctg gttactgttg
     cgcctgttag cgcggcaacg tccggcgcac agaagctatt atgcgtcccc aggtaatgaa
     taattgcctc tttgcccgtc atacacttgc tcctttcagt ccgaacttag ctttgatttc
     tgcgatcttc gccagagcct gtgcacgatt tagaggtcta ccgcccatga caggaagttg
     ttttactggt tcagggatcg cctcaccacg gttaattctc gcagtcatat ggacaagctc
     atctgcggcc ttacggcgta attccgcatc agtaagcgca ttggcccgca tgttctgata
     caggttggta accagccagt agtgcgcgtt tgatttccac ggataagact ccgcatccgg
     atacaggcct cgcttccggc aatactcgta aaccatatca accagctcgc tgacgtttgg
     cagtccggcg gtaacggatg cttcttcccg gcaccatgca acaaactgcc cgggtgatgg
     cagaaatggt cgattctgcc gacgggctac gcgcattcct gcgttaacct gttccatcgt
     ggtgatcccg ttttcccgaa aagccagaac ccactggcga cggatttcgt tcacttcgtt
     ctggtcacgg ttagccaggc tcgccgggaa agttgccagt aactggctga acacaccgtt
     gatgatctgc gctacctgct gtacctgcgg cttttcgtcg tactgttccg gcatgttgtt
     ggcgatccga cgcatctgct cacggtcaaa gttaaccatc tgtgcggcga tgtttttcat
     agatccaccc cgtaaatcca gtctgtgttt gtcaggtcga gttttggttt gctggctgtc
     acgcctgcct gttgcttgtt acggttgatt tcgagttggg tccacttatc gcggagtttg
     gccgggctca gcacgttacc ggaccagaag ttgtcctggc atgcccagcg gaacagcaca
     cacatgtcgc ggtggttacg tccgtcacgt tcacgcatca ggcggatatc gttagcccac
     ccagcaaaat tcggttttct ggctgatggt gcgatagtct tcaccatgtc aaacatccac
     tctgcggcgg tcaggtcttc tgctgtcccc cacttgctgc cgctctgaat tgcagcatcc
     ggtttcacca cagaaaggtc gttttctggc tggtcagagg attcgccaga attctctgac
     gaataatctt ttcttttttc ttttgtaata gtgtcttttg tgtccccctg ttttgaggga
     tagcaatccc ccaatttgag ggatgtttta tccctcgttt taggggattt tccctcgttt
     tgagggatgc accattctga gatgttttta tttggtccaa acatgccgcc ttgctgcttg
     ataatattca ttctgacgag ttctaacttg gcttcattgc accgtttgac aggtaacttt
     gtaatctcgc taagttgaga atcggtgatt ctgtccattg gtttattcca cccataggtt
     ttacgcagaa tggcaagcag cactttaaac tgtcgcttgg tcagatctgc gcccgaataa
     gcctcaagca gcatatttga tagtctggcg taaccatcat cgagatctgc cacattacgc
     tcctgtccgg caaagttacc tctgccgaag ttgagtattt ttgctgtatt tgtcataatg
     actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
     cggttgccgc cgggcgtttt ttattggtga gaatcgcagc aacttgtcgc gccaatcgag
     ccatgtcgtc gtcaacgacc ccccattcaa gaacagcaag cagcattgag aactttggaa
     tccagtccct cttccacctg ctgatctgcg acttatcaac gcccacagct tccgctgtct
     tctcagttcc aagcattgcg attttgttaa gcaacgcact ctcgattcgt agagcctcgt
     tgcgtttgtt tgcacgaacc atatgtaagt atttccttag ataacaattg attgaatgta
     tgcaaataaa tgcatacacc ataggtgtgg tttaatttga tgcccttttt cagggctgga
     atgtgtaaga gcggggttat ttatgctgtt gtttttttgt tactcgggaa gggctttacc
     tcttccgcat aaacgcttcc atcagcgttt atagttaaaa aaatctttcg gcctgcatga
     atggccttgt tgatcgcgct ttgatatacg ccgagatctt tagctgtctt ggtttgccca
     aagcgcattg cataatcttt cagggttatg cgttgttcca tacaacctcc ttagtacatg
     caaccattat caccgccaga ggtaaaatag tcaacacgca cggtgttaga tatttatccc
     ttgcggtgat agatttaacg tatgagcaca aaaaagaaac cattaacaca agagcagctt
     gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
     caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
     ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
     gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
     atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
     atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
     accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
     ccaacaggct ccaagccaag ctagcttcac gctgccgcaa gcactcaggg cgcaagggct
     gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa acggtgctga ccccggatga
     atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag
     cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac
     cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
     tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag
     gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     catgctggag ttcttcgccc accccgggct cgatcccctc gcgagttggt tcagctgctg
     cctgaggctg gacgacctcg cggagttcta ccggcagtgc aaatccgtcg gcatccagga
     aaccagcagc ggctatccgc gcatccatgc ccccgaactg caggagtggg gaggcacgat
     ggccgctttg gtcccggatc aattcgcgcg accg
//