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ID   LHDCX      preliminary; circular DNA; SYN; 6837 BP.
XX
AC   M64754;
XX
DT   09-MAY-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse Moloney leukemia retrovirus vector MMLV LHDCX - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6837
RC   LHDCX from MMLV
RC   LXSHD from MMLV
RA   Stockshlaeder M.A., Storb R., Osborne W.R., Miller A.D.;
RT   "L-histidinol provides effective selection of
RT   retrovirus-vector-transduced keratinocytes without impairing their
RT   proliferative potential";
RL   Hum. Gene Ther. 2:33-39(1991).
XX
RN   [2]
RC   pLHMlPL from pLPL & mouse MT promoter & human PNP gene
RC   pLHMlAL from pLPL & mouse MT promoter & human ADA gene
RC   pLHMsPL from pLPL & mouse MT promoter & human PNP gene
RC   pLHMsAL from pLPL & mouse MT promoter & human ADA gene
RC   LHMlPL from pLHMlPL
RC   LHMlAL from pLHMlAL
RC   LHMsPL from pLHMsPL
RC   LHMsAL from pLHMsAL
RA   McIvor R.S., Johnson M.J., Miller A.D., Pitts S., Williams S.R.,
RA   Valerio D., Martin D.W., Verma I.M.;
RT   "Human purine nucleoside phosphorylase and adenosine deaminase:
RT   gene transfer into cultured cells and murine hematopoietic stem
RT   cells by using recombinant amphotropic retroviruses";
RL   Mol. Cell. Biol. 7:838-846(1987).
XX
RN   [3]
RC   LNEAL from MoMLV env gene & SV40 early promoter & human ADA gene
RC   LDEAL from MoMLV env gene & SV40 early promoter & human ADA gene
RA   McIvor R.S., Pitts S., Martin D.W.;
RT   ;
RL   New Approaches to Genetic Diseases 0:231-244(1987).
RL   Sasazuki T., ed., Academic, Tokyo.
XX
RN   [4]
RC   pLPL from pBR322 & human HPRT gene & MoMLV LTR & MoMSV LTR
RA   Miller A.D., Jolly D.J., Friedmann T., Verma I.M.;
RT   "A transmissible retrovirus expressing human hypoxanthine
RT   phosphoribosyltransferase (HPRT); gene transfer into cells
RT   obtained from humans deficient in HPRT";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:4709-4713(1983).
XX
RN   [5]
RC   pFR400 from mouse DHFR gene
RA   Simonsen C.C., Levinson A.D.;
RT   "Isolation and expression of an altered mouse dihydrofolate
RT   reductase cDNA";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499(1983).
XX
RN   [6]
RC   pPAM from neo gene & MoMULV
RA   Miller A.D., Law M.F., Verma I.M.;
RT   "Generation of helper-free amphotropic retroviruses that transduce
RT   a dominant-acting, methotrexate-resistant dihydrofolate reductase
RT   gene";
RL   Mol. Cell. Biol. 5:431-437(1985).
XX
CC   NM (LHDCX)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (mouse)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Moloney murine leukemia virus)(Moloney murine sarcoma virus)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. LNCX remove 1100bp, neo gene
FT                   \ 1656..2450/6620bp
FT                   2. E. coli 1354bp, hisD gene
FT                   -> LHDCX 6837bp"
FT   misc_recomb     174..175
FT                   /note="mouse DNA end/Moloney murine sarcoma virus
FT                   (Mo-MuSV) DNA start"
FT   LTR             175..763
FT                   /note="RPT Moloney murine sarcoma virus (Mo-MuSV)
FT                   5' long terminal repeat"
FT   misc_signal     833..1642
FT                   /note="extended packaging signal"
FT   misc_recomb     1163..1164
FT                   /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT                   end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT   mutation        1223..1225
FT                   /note="GAG atg start codon to TAG stop codon"
FT   misc_recomb     1662..1663
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/E. coli hisD DNA start"
FT   CDS             1672..2976
FT                   /note="ANT E. coli histidinol dehydrogenase gene
FT                   histidinol resistance gene (his)"
FT   misc_recomb     3016..3017
FT                   /note="E. coli hisD DNA end/human cytomegalavirus
FT                   start"
FT   promoter        3017..3834
FT                   /note="PRO human cytomegalovirus early gene"
FT   misc_recomb     3828..3829
FT                   /note="human cytomegalovirus DNA end/Moloney murine
FT                   leukemia virus (Mo-MuLV) DNA start"
FT   LTR             3883..4476
FT                   /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT                   3' long terminal repeat"
FT   misc_recomb     4545..4546
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/plasmid pBR322 DNA start"
XX
SQ   Sequence 6837 BP; 1610 A; 1877 C; 1722 G; 1628 T; 0 other;
     gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
     tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
     ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
     agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
     atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
     ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
     acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
     gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
     gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
     atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
     cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
     tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
     tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
     tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
     ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
     ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
     acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     cctcactcct tctctaggcg ccggaattcg ttaactcgag gatcccggac catgagcttc
     aataccctga ttgactggaa cagctgtagc cctgaacagc agcgtgcgct gctgacgcgt
     ccggcgattt ccgcctctga cagtattacc cggacggtca gcgatattct ggataatgta
     aaaacgcgcg gtgacgatgc cctgcgtgaa tacagcgcta aatttgataa aacagaagtg
     acagcgctac gcgtcacccc tgaagagatc gccgccgccg gcgcgcgtct gagcgacgaa
     ttaaaacagg cgatgaccgc tgccgtcaaa aatattgaaa cgttccattc cgcgcagacg
     ctaccgcctg tagatgtgga aacccagcca ggcgtgcgtt gccagcaggt tacgcgtccc
     gtctcgtctg tcggtctgta tattcccggc ggctcggctc cgctcttctc aacggtgctg
     atgctggcga cgccggcgcg cattgcggga tgccagaagg tggttctgtg ctcgccgccg
     cccatcgctg atgaaatcct ctatgcggcg caactgtgtg gcgtgcagga aatctttaac
     gtcggcggcg cgcaggcgat tgccgctctg gccttcggca gcgagtccgt accgaaagtg
     gataaaattt ttggccccgg caacgccttt gtaaccgaag ccaaacgtca ggtcagccag
     cgtctcgacg gcgcggctat cgatatgcca gccgggccgt ctgaagtact ggtgatcgca
     gacagcggcg caacaccgga tttcgtcgct tctgacctgc tctcccaggc tgagcacggc
     ccggattccc aggtgatcct gctgacgcct gatgctgaca ttgcccgcaa ggtggcggag
     gcggtagaac gtcaactggc ggaactgccg cgcgcggaca ccgcccggca ggccctgagc
     gccagtcgtc tgattgtgac caaagattta gcgcagtgcg tcgccatctc taatcagtat
     gggccggaac acttaatcat ccagacgcgc aatgcgcgcg atttggtgga tgcgattacc
     agcgcaggct cggtatttct cggcgactgg tcgccggaat ccgccggtga ttacgcttcc
     ggaaccaacc atgttttacc gacctatggc tatactgcta cctgttccag ccttgggtta
     gcggatttcc agaaacggat gaccgttcag gaactgtcga aagcgggctt ttccgctctg
     gcatcaacca ttgaaacatt ggcggcggca gaacgtctga ccgcccataa aaatgccgtg
     accctgcgcg taaacgccct caaggagcaa gcatgagcac tgaaaacact ctcagcgtcg
     ctgacttagc ccgggatccg gccattagcc atattattca ttggttatat agcataaatc
     aatattggct attggccatt gcatacgttg tatccatatc ataatatgta catttatatt
     ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
     tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc atgtacggtg ggaggtctat
     ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccaagct tgttaacatc
     gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
     tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
     tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag
     acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt
     cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaa
//