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ID LHDCX preliminary; circular DNA; SYN; 6837 BP.
XX
AC M64754;
XX
DT 09-MAY-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Mouse Moloney leukemia retrovirus vector MMLV LHDCX - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-6837
RC LHDCX from MMLV
RC LXSHD from MMLV
RA Stockshlaeder M.A., Storb R., Osborne W.R., Miller A.D.;
RT "L-histidinol provides effective selection of
RT retrovirus-vector-transduced keratinocytes without impairing their
RT proliferative potential";
RL Hum. Gene Ther. 2:33-39(1991).
XX
RN [2]
RC pLHMlPL from pLPL & mouse MT promoter & human PNP gene
RC pLHMlAL from pLPL & mouse MT promoter & human ADA gene
RC pLHMsPL from pLPL & mouse MT promoter & human PNP gene
RC pLHMsAL from pLPL & mouse MT promoter & human ADA gene
RC LHMlPL from pLHMlPL
RC LHMlAL from pLHMlAL
RC LHMsPL from pLHMsPL
RC LHMsAL from pLHMsAL
RA McIvor R.S., Johnson M.J., Miller A.D., Pitts S., Williams S.R.,
RA Valerio D., Martin D.W., Verma I.M.;
RT "Human purine nucleoside phosphorylase and adenosine deaminase:
RT gene transfer into cultured cells and murine hematopoietic stem
RT cells by using recombinant amphotropic retroviruses";
RL Mol. Cell. Biol. 7:838-846(1987).
XX
RN [3]
RC LNEAL from MoMLV env gene & SV40 early promoter & human ADA gene
RC LDEAL from MoMLV env gene & SV40 early promoter & human ADA gene
RA McIvor R.S., Pitts S., Martin D.W.;
RT ;
RL New Approaches to Genetic Diseases 0:231-244(1987).
RL Sasazuki T., ed., Academic, Tokyo.
XX
RN [4]
RC pLPL from pBR322 & human HPRT gene & MoMLV LTR & MoMSV LTR
RA Miller A.D., Jolly D.J., Friedmann T., Verma I.M.;
RT "A transmissible retrovirus expressing human hypoxanthine
RT phosphoribosyltransferase (HPRT); gene transfer into cells
RT obtained from humans deficient in HPRT";
RL Proc. Natl. Acad. Sci. U.S.A. 80:4709-4713(1983).
XX
RN [5]
RC pFR400 from mouse DHFR gene
RA Simonsen C.C., Levinson A.D.;
RT "Isolation and expression of an altered mouse dihydrofolate
RT reductase cDNA";
RL Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499(1983).
XX
RN [6]
RC pPAM from neo gene & MoMULV
RA Miller A.D., Law M.F., Verma I.M.;
RT "Generation of helper-free amphotropic retroviruses that transduce
RT a dominant-acting, methotrexate-resistant dihydrofolate reductase
RT gene";
RL Mol. Cell. Biol. 5:431-437(1985).
XX
CC NM (LHDCX)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (mouse)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (Moloney murine leukemia virus)(Moloney murine sarcoma virus)(SV40)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. LNCX remove 1100bp, neo gene
FT \ 1656..2450/6620bp
FT 2. E. coli 1354bp, hisD gene
FT -> LHDCX 6837bp"
FT misc_recomb 174..175
FT /note="mouse DNA end/Moloney murine sarcoma virus
FT (Mo-MuSV) DNA start"
FT LTR 175..763
FT /note="RPT Moloney murine sarcoma virus (Mo-MuSV)
FT 5' long terminal repeat"
FT misc_signal 833..1642
FT /note="extended packaging signal"
FT misc_recomb 1163..1164
FT /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT mutation 1223..1225
FT /note="GAG atg start codon to TAG stop codon"
FT misc_recomb 1662..1663
FT /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT end/E. coli hisD DNA start"
FT CDS 1672..2976
FT /note="ANT E. coli histidinol dehydrogenase gene
FT histidinol resistance gene (his)"
FT misc_recomb 3016..3017
FT /note="E. coli hisD DNA end/human cytomegalavirus
FT start"
FT promoter 3017..3834
FT /note="PRO human cytomegalovirus early gene"
FT misc_recomb 3828..3829
FT /note="human cytomegalovirus DNA end/Moloney murine
FT leukemia virus (Mo-MuLV) DNA start"
FT LTR 3883..4476
FT /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT 3' long terminal repeat"
FT misc_recomb 4545..4546
FT /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT end/plasmid pBR322 DNA start"
XX
SQ Sequence 6837 BP; 1610 A; 1877 C; 1722 G; 1628 T; 0 other;
gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
cctcactcct tctctaggcg ccggaattcg ttaactcgag gatcccggac catgagcttc
aataccctga ttgactggaa cagctgtagc cctgaacagc agcgtgcgct gctgacgcgt
ccggcgattt ccgcctctga cagtattacc cggacggtca gcgatattct ggataatgta
aaaacgcgcg gtgacgatgc cctgcgtgaa tacagcgcta aatttgataa aacagaagtg
acagcgctac gcgtcacccc tgaagagatc gccgccgccg gcgcgcgtct gagcgacgaa
ttaaaacagg cgatgaccgc tgccgtcaaa aatattgaaa cgttccattc cgcgcagacg
ctaccgcctg tagatgtgga aacccagcca ggcgtgcgtt gccagcaggt tacgcgtccc
gtctcgtctg tcggtctgta tattcccggc ggctcggctc cgctcttctc aacggtgctg
atgctggcga cgccggcgcg cattgcggga tgccagaagg tggttctgtg ctcgccgccg
cccatcgctg atgaaatcct ctatgcggcg caactgtgtg gcgtgcagga aatctttaac
gtcggcggcg cgcaggcgat tgccgctctg gccttcggca gcgagtccgt accgaaagtg
gataaaattt ttggccccgg caacgccttt gtaaccgaag ccaaacgtca ggtcagccag
cgtctcgacg gcgcggctat cgatatgcca gccgggccgt ctgaagtact ggtgatcgca
gacagcggcg caacaccgga tttcgtcgct tctgacctgc tctcccaggc tgagcacggc
ccggattccc aggtgatcct gctgacgcct gatgctgaca ttgcccgcaa ggtggcggag
gcggtagaac gtcaactggc ggaactgccg cgcgcggaca ccgcccggca ggccctgagc
gccagtcgtc tgattgtgac caaagattta gcgcagtgcg tcgccatctc taatcagtat
gggccggaac acttaatcat ccagacgcgc aatgcgcgcg atttggtgga tgcgattacc
agcgcaggct cggtatttct cggcgactgg tcgccggaat ccgccggtga ttacgcttcc
ggaaccaacc atgttttacc gacctatggc tatactgcta cctgttccag ccttgggtta
gcggatttcc agaaacggat gaccgttcag gaactgtcga aagcgggctt ttccgctctg
gcatcaacca ttgaaacatt ggcggcggca gaacgtctga ccgcccataa aaatgccgtg
accctgcgcg taaacgccct caaggagcaa gcatgagcac tgaaaacact ctcagcgtcg
ctgacttagc ccgggatccg gccattagcc atattattca ttggttatat agcataaatc
aatattggct attggccatt gcatacgttg tatccatatc ataatatgta catttatatt
ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc atgtacggtg ggaggtctat
ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccaagct tgttaacatc
gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag
acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt
cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaa
//