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ID   LN         preliminary; circular DNA; SYN; 5464 BP.
XX
AC   M28245;
XX
DT   28-JAN-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse Moloney murine sarcoma virus vector LN - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5464
RC   LN, LNSX, LNCX, LXSN from LNL6
RA   Miller A.D.;
RT   ;
RL   Unpublished (1989).
XX
RN   [2]
RC   LN, LNSX, LNCX, LXSN from LNL6
RA   Miller A.D., Rosman G.J.;
RT   "Improved retroviral vectors for gene transfer and expression";
RL   Biotechniques 7:980-990(1989).
XX
RN   [3]
RP   1-5464
RC   LN
RA   Miller A.D.;
RT   ;
RL   Submitted (22-SEP-1989) to GenBank on computer-readable media by:
RL   Miller A.D., Program in Molecular Medicine,
RL   Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
XX
RN   [4]
RC   LN, LNSX, LNCX, LXSN from LNL6
RA   Kuebbing D., Freidman S.;
RT   ;
RL   Unpublished (1991).
XX
CC   NCBI gi: 208842
CC   NM (LN)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (mouse)
CC   CP ()
CC   FN (gene transfer)(expression)
CC   SE ()
CC   PA (LNL6)
CC   BR (LNCX)(LNSX)(LXSN)
CC   OF ()
CC   OR (1 bp 5' to EcoRI site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. LNL6 remove 681bp,
FT                   \ MMLV LTR/packaging/neo/LTR/polyA/5464bp
FT                   -> LN 5464bp"
FT   misc_recomb     144..145
FT                   /note="mouse DNA end/Moloney murine sarcoma virus
FT                   (Mo-MuSV) DNA start"
FT   LTR             145..733
FT                   /note="RPT Moloney murine sarcoma virus (Mo-MuSV)
FT                   5' long terminal repeat"
FT   misc_signal     803..1612
FT                   /note="extended packaging signal"
FT   misc_recomb     1133..1134
FT                   /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT                   end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT   mutation        1193..1195
FT                   /note="GAG atg start codon to TAG stop codon"
FT   misc_recomb     1616..1617
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/transposon Tn5 DNA start"
FT   CDS             1656..2450
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)"
FT   misc_recomb     2467..2468
FT                   /note="transposon Tn5 DNA end/Moloney murine leukemia
FT                   virus (MoMuLV) DNA start"
FT   LTR             2510..3103
FT                   /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT                   3' long terminal repeat"
FT   misc_recomb     3172..3173
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/plasmid pBR322 DNA start"
XX
SQ   Sequence 5464 BP; 1256 A; 1496 C; 1410 G; 1302 T; 0 other;
     gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
     tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
     ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
     aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
     caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
     tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
     tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
     cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
     aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
     cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
     gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
     ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
     ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
     tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
     gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
     ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
     cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
     catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
     gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
     tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
     gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
     ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
     cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
     taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
     gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
     gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     ggggttcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
     tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg
     cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     tcaa
//