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ID   LNCX       preliminary; circular DNA; SYN; 6620 BP.
XX
AC   M28247;
XX
DT   28-JAN-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse Moloney murine sarcoma virus vector LNCX - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6620
RC   LN, LNSX, LNCX, LXSN from LNL6
RA   Miller A.D.;
RT   ;
RL   Unpublished (1989).
XX
RN   [2]
RC   LN, LNSX, LNCX, LXSN from LNL6
RA   Miller A.D., Rosman G.J.;
RT   "Improved retroviral vectors for gene transfer and expression";
RL   Biotechniques 7:980-990(1989).
XX
RN   [3]
RP   1-6620
RC   LNCX
RA   Miller A.D.;
RT   ;
RL   Submitted (22-SEP-1989) to GenBank on computer-readable media by:
RL   Miller A.D., Program in Molecular Medicine,
RL   Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
XX
RN   [4]
RC   LN, LNSX, LNCX, LXSN from LNL6
RA   Kuebbing D., Freidman S.;
RT   ;
RL   Unpublished (1991).
XX
CC   NCBI gi: 208846
CC   NM (LNCX)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (mouse)
CC   CP ()
CC   FN (gene transfer)(expression)
CC   SE ()
CC   PA (LNL6)
CC   BR (LN)(LNSX)(LXSN)
CC   OF ()
CC   OR (1 bp 5' to EcoRI site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. LNL6 remove 681bp,
FT                   \ MMLV LTR/packaging/neo/LTR/polyA/5464bp
FT                   2. CMV 1138bp, immediate early promoter
FT                   3. linker HindIII-HpaI-ClaI 18bp aagcttgttaacatcgat
FT                   -> LNCX 6620bp"
FT   misc_recomb     144..145
FT                   /note="mouse DNA end/Moloney murine sarcoma virus
FT                   (Mo-MuSV) DNA start"
FT   LTR             145..733
FT                   /note="RPT Moloney murine sarcoma virus (Mo-MuSV)
FT                   5' long terminal repeat"
FT   misc_signal     803..1612
FT                   /note="extended packaging signal"
FT   misc_recomb     1133..1134
FT                   /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT                   end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT   mutation        1193..1195
FT                   /note="GAG atg start codon to TAG stop codon"
FT   misc_recomb     1616..1617
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/transposon Tn5 DNA start"
FT   CDS             1656..2450
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)"
FT   misc_recomb     2799..2800
FT                   /note="transposon Tn5 DNA end/human cytomegalovirus
FT                   DNA start"
FT   promoter        2800..3617
FT                   /note="PRO human cytomegalovirus immediate early gene"
FT   misc_recomb     3617..3618
FT                   /note="human cytomegalovirus DNA end/Moloney murine
FT                   leukemia virus (Mo-MuLV) DNA start"
FT   LTR             3666..4259
FT                   /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT                   3' long terminal repeat"
FT   misc_recomb     4328..4329
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/plasmid pBR322 DNA start"
XX
SQ   Sequence 6620 BP; 1528 A; 1807 C; 1692 G; 1593 T; 0 other;
     gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
     tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
     ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
     aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
     caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
     tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
     tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
     cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
     aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
     cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
     gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
     ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
     ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
     tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
     gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
     ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
     cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
     catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
     gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
     tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
     gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
     ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
     cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
     taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
     gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
     gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
     gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
     atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
     ggagtgggga ggcacgatgg ccgctttggt cgaggcggat ccggccatta gccatattat
     tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatccat
     atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
     tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     ggcatgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     gcggccccaa gcttgttaac atcgataaaa taaaagattt tatttagtct ccagaaaaag
     gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
     gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
     taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     cgaggccctt tcgtcttcaa
//