back Return to this vector's summary.
ID   LNL6       preliminary; circular DNA; SYN; 6145 BP.
XX
AC   M63653;
XX
DT   25-FEB-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse Moloney leukemia retrovirus vector LNL6 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   LHL2 from hph gene & MoMuLV
RC   pLNL from N2
RC   LNL-XHC from pLNL & linker
RC   LNL6 from N2
RA   Bender M.A., Palmer T.D., Gelinas R.E., Miller A.D.;
RT   "Evidence that the packaging signal of moloney murine leukemia virus
RT   extends into the gag region";
RL   J. Virol. 61:1639-1646(1987).
XX
RN   [2]
RP   1-300
RC   LNL6 from N2
RA   Kuebbing D., Friedman S., Jacobson M.;
RT   ;
RL   Unpublished (1989).
XX
RN   [3]
RP   301-6145
RC   LNL6 from N2
RA   Miller A.D.;
RT   ;
RL   Unpublished (1989).
XX
RN   [4]
RP   1-6145
RC   LNL6
RA   Miller A.D.;
RT   ;
RL   Submitted (25-FEB-1992) by:
RL   Miller A.D., Program in Molecular Medicine,
RL   Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
XX
RN   [5]
RC   N2 from MoMuLV 5' LTR & Tn5 & MoMuLV 3'LTR
RC   N3 from MoMuLV 5' LTR & Tn5 & MoMuLV 3'LTR
RC   pXm5 from N2 & MMLV LTR/gag & neo gene
RA   Keller G., Paige P., Gilboa E., Wagner E.F.;
RT   "Expression of a foreign gene in myeloid and lymphoid cells
RT   derived from multipotent haematopoietic precursors";
RL   Nature 318:149-154(1985).
XX
RN   [6]
RC   LHL from MoMULV LTR & pLG90, hph gene
RC   LHL2 from MoMUSV LTR & pLG90, hph gene
RC   LSHL from MoMUSV LTR & pLG90, hph gene
RC   N2 from MoMULV LTR & neo gene
RC   LNSAL from N2 & ADA gene
RA   Palmer T.D., Hock R.A., Osborne W.R., Miller A.D.;
RT   "Efficient retrovirus-mediated transfer and expression of a human
RT   adenosine deaminase gene in diploid skin fibroblasts from an
RT   adenosine deaminase-deficient human";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:1055-1059(1987).
XX
RN   [7]
RC   SDHT from SFFV & pFR400, dhfr gene
RC   N2 from neo gene & MoMULV
RC   PAM from neo gene & MoMULV
RC   LNL2 from Tn5 neo gene & MoMULV
RA   Miller A.D., Trauber D.R., Buttimore C.;
RT   "Factors involved in the production of helper virus-free
RT   retrovirus vectors";
RL   Somatic Cell Mol. Genet. 12:175-183(1986).
XX
CC   NCBI gi: 208850
CC   NM (LNL6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (mouse)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (N2)(MoMSV)
CC   BR ()
CC   OF (LN)(LNCX)(LNSX)(LXSN)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. MoMuLV BglI-XhoI, 5' LTR
FT                   BAL31 nuclease
FT                   EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT                   2. Tn5 BglII-BamHI 1541bp 1516..3057, neo gene
FT                   EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT                   3. MoMuLV BamHI-PstI, 3' LTR
FT                   BAL31 nuclease
FT                   EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT                   -> N2
FT                   1. MoMuLV remove 1039..7673, 1698bp
FT                   2. Tn5 BglII-BamHI 1541bp 1516..3057, neo gene
FT                   -> N2 3239bp [MoMuLV LTR]
FT                   1. N2 complement ATG
FT                   -> LNL6 6145bp"
FT   misc_recomb     144..145
FT                   /note="mouse DNA end/Moloney murine sarcoma virus
FT                   (Mo-MuSV) DNA start"
FT   LTR             145..733
FT                   /note="Moloney murine sarcoma virus (Mo-MuSV)
FT                   5' long terminal repeat"
FT   misc_signal     803..1612
FT                   /note="extended packaging signal"
FT   misc_recomb     1133..1134
FT                   /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT                   end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT   mutation        1193..1195
FT                   /note="GAG atg start codon to TAG stop codon"
FT   misc_recomb     1616..1617
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/transposon Tn5 DNA start"
FT   CDS             1656..2450
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)"
FT   misc_recomb     3051..3052
FT                   /note="transposon Tn5 DNA end/Moloney murine
FT                   leukemia virus (MoMuLV) DNA start"
FT   LTR             3191..3784
FT                   /note="Moloney murine leukemia virus (Mo-MuLV)
FT                   3' long terminal repeat"
FT   misc_recomb     3853..3854
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/plasmid pBR322 DNA start"
XX
SQ   Sequence 6145 BP; 1397 A; 1700 C; 1607 G; 1441 T; 0 other;
     gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
     tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
     ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
     aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
     caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
     tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
     tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
     cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
     aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
     cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
     gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
     ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
     ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
     tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
     gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
     ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
     cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
     catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
     gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
     tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
     gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
     ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
     cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
     taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
     gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
     gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
     gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
     atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
     ggagtgggga ggcacgatgg ccgctttggt cgacccggac gggacgctcc tgcgcctgat
     acagaacgaa ttgcttgcag gcatctcatg agtgtgtctt cccgttttcc gcctgaggtc
     actgcgtgga tggagcgctg gcgcctgctg cgcgacggcg agctgctcac cacccactcg
     agggcgtgca gcgctgcaga ggccgagtgc agaactgctc caaagggacc tcaaggcttt
     ccgagggaca ctaggctgac tccatcgagc cagtgtagag ataagcttat cgattagtcc
     aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
     aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg
     ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
     catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     tatcacgagg ccctttcgtc ttcaa
//