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ID   LORDEL     preliminary; circular DNA; SYN; 5065 BP.
XX
AC   IG9926;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector Lordel - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUCSP from pUC12 & pSP64, Sp6 promoter
RC   pUCT7 from pUC12 & pT7-2, T7 promoter
RC   Lorus from Loric & pUCSP
RC   Lordel from Lorus
RC   LordelO from Lordel
RC   LordelB from LordelO
RC   LdBp12 from LordelB & pUC12
RC   Loris from LdBp12
RC   pUCT7Bdelta from pUCT7
RC   LorspT7 from Loris & pUCT7Bdelta
RC   LoristB from LorspT7
RA   Cross S.H., Little P.F.;
RT   "A cosmid vector for systematic chromosome walking";
RL   Gene 49:9-22(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (Lordel)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Loric)(pSP64)(pUC12)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 remove BamHI-HindIII 27bp 249..276,
FT                   \ MCS/2653bp
FT                   2. pSP64 Sau3AI/MboI-HindIII 256bp 2751..2999..8,
FT                   \ Sp6 promoter
FT                   -> pUCSP 2909bp
FT                   1. Loric ClaI-HindIII 6192bp 300..6492
FT                   Klenow:
FT                   2. pUCSP EcoRI-HindIII 274bp 231..249 - 276,
FT                   \ Sp6 promoter
FT                   \ pUC12 231..276
FT                   \ pSP64 ..8
FT                   Klenow:
FT                   -> Lorus 6466bp
FT                   1. Lorus remove SspI-SstII/SacII 1411bp,
FT                   \ Loric 1279..2690/5055bp
FT                   T4 DNA polymerase
FT                   -> Lordel 5055bp [5193bp]"
FT   -               1..979
FT                   /note="Loric 300..1278 979bp
FT                   SspI = AAT^ATT
FT                   SacII = CC GC^GG"
FT   -               980..4787
FT                   /note="Loric 2684..6491 3808bp
FT                   HindIII = A^AGCTT"
FT   -               4788..5047
FT                   /note="pSP64 2751..2999..11 260bp complement
FT                   MboI = Sau3AI = ^GATC
FT                   BamHI =        G^GATCC"
FT   -               5048..5065
FT                   /note="pUC12 231..248 18bp complement
FT                   EcoRI = G^AATT C
FT                   ClaI =      AT^CGAT"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5065 BP; 1152 A; 1310 C; 1287 G; 1313 T; 3 other;
     cgataaatgg cttcagaaca ggcattcgcg tctgaatatc ctttggttcc cataccgtat
     aaccatttgg ctgtccaagc tccgggttga tatcaacctg caatacggtg agcggtatat
     cccagaactt cacaacttcc ctgacaaacc gatatgtcat tggatgttca caacctgtat
     ccatgaaaac gtaatgcacg tctttacctg cccgtcgctt ttgctccatt agccagagca
     aatatgctga cgtcctgcca ccggagaaac taacgacatt tatcatgcag ccctgtctcc
     ccatctcgct ttccactcca gagccagtct cgcttcgtct gaccacttaa cgccacgctc
     tgtaccgaat gcctgtataa gctctaatag ctccgcaaat tcgcctacac gcatcctgct
     ggttgactgg cctattacca caaagccatt cccggcaagg ttaggaacaa catcctgctg
     ctttaatgct gcggtaaaca cacacttcca gctttctgca tccagccagc gaccatgcca
     ttcaacctga cgagagacgt cacctaagca ggcccatagc ttcctgtttt ggtctaagct
     gcggttgcgt tcctgaatgg ttactacgat tggtttggtt gggtctggaa ggatttgctg
     tactgcgtga atagcgtttt gctgatgtgc tggagatcga atttcaaagg ttagtttttt
     catgacttcc ctctccccca aataaaaagg cctgcgatta ccagcaggcc tgttattagc
     tcagtaatgt agatggtcat cttttaactc catataccgc caatacccgt ttcatcgcgg
     cactctggcg acactcctta aaaaccaggt tcgtgctcat ctttccttcc cgttcttccc
     tggtagcaaa ccggtaatac accgttcgcc agaccttacc ttcgataacc agaagacctg
     cccgtgccat tttagccgcg ataatattca ttctgacgag ttctaacttg gcttcattgc
     accgtttgac aggtaacttt gtaatctcgc taagttgaga atcggtgatt ctgtccattg
     gtttattcca cccataggtt ttacgcagaa tggcaagcag cactttaaac tgtcgcttgg
     tcagatctgc gcccgaataa gcctcaagca gcatatttga tagtctggcg taaccatcat
     cgagatctgc cacattacgc tcctgtccgg caaagttacc tctgccgaag ttgagtattt
     ttgctgtatt tgtcataatg actcctgttg atagatccag taatgacctc agaactccat
     ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatcgcagc
     aacttgtcgc gccaatcgag ccatgtcgtc gtcaacgacc ccccattcaa gaacagcaag
     cagcattgag aactttggaa tccagtccct cttccacctg ctgatctgcg acttatcaac
     gcccacagct tccgctgtct tctcagttcc aagcattgcg attttgttaa gcaacgcact
     ctcgattcgt agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttag
     ataacaattg attgaatgta tgcaaataaa tgcatacacc ataggtgtgg tttaatttga
     tgcccttttt cagggctgga atgtgtaaga gcggggttat ttatgctgtt gtttttttgt
     tactcgggaa gggctttacc tcttccgcat aaacgcttcc atcagcgttt atagttaaaa
     aaatctttcg gcctgcatga atggccttgt tgatcgcgct ttgatatacg ccgagatctt
     tagctgtctt ggtttgccca aagcgcattg cataatcttt cagggttatg cgttgttcca
     tacaacctcc ttagtacatg caaccattat caccgccaga ggtaaaatag tcaacacgca
     cggtgttaga tatttatccc ttgcggtgat agatttaacg tatgagcaca aaaaagaaac
     cattaacaca agagcagctt gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga
     aaaatgaact tggcttatcc caggaatctg tcgcagacaa gatggggatg gggcagtcag
     gcgttggtgc tttatttaat ggcatcaatg cattaaatgc ttataacgcc gcattgcttg
     caaaaattct caaagttagc gttgaagaat ttagcccttc aatcgccaga gaaatctacg
     agatgtatga agcggttagt atgcagccgt cacttagaag tgagtatgag taccctgttt
     tttctcatgt tcaggcaggg atgttctcac ctgagcttag aacctttacc aaaggtgatg
     cggagagatg ggtaagcaca accaaaaaag ccagtgattc tgcattctgg cttgaggttg
     aaggtaattc catgaccgca ccaacaggct ccaagccaag ctagcttcac gctgccgcaa
     gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa
     acggtgctga ccccggatga atgtcactac tgggctatct ggacaaggga aaacgcaagc
     gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
     ttatggacag caagcgaacc ggaattgcca gctggggcgc catctggtaa ggttgggaag
     ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgagcagcg cggctatcgt
     ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     gggactggct gctattgggc gaagtgccgg ggcaggatct ccagtcatct caccttgctc
     ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggctgg
     aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgcgatt tcgattccac
     cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
     gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
     atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
     ggagtgggga ggcacgatgg ccgctttggt cgaccggacg ggacgctcct gcgcctgata
     cagaacgaat tgcttgcagg catctcatga gtgtgtcttc ccgttttccg cctgaggtca
     ctgcgtggat ggagcgctgg cgcctgctgc gcgacggcga gctgctcacc acccactcga
     gctggatact tcgcgtccgc cagggggaca tgccggcgat gctgaaggtc gcccgcattc
     ccgatgaaga ggccggttac cgcctgttga cctggtggga cgggcagggc gccccccgac
     tcttcgcctc ggcggcgggc gctctgctca tggagcgcgc gtccggggcc ggggaccttg
     cacagatagc gtggtccggc caggacgacg aggcttgcag gatcaattcn ggatccnntt
     gacatgaggt tgccccgtat tcagtgtcgc tgatttgtat tgtctgaagt tgtttttacg
     ttaagttgat gcagatcaat taatacgata cctgcgtcat aattgattat ttgacgtggt
     ttgatggcct ccacgcacgt tgtgatatgt agatgataat cattatcact ttacgggtcc
     tttccggtga tccgacaggt tacggggcgg cgacctcgcg ggttttcgct atttatgaaa
     attttccggt ttaaggcgtt tccgttcttc ttcgtcataa cttaatgttt ttatttaaaa
     taccctctga aaagaaagga aacgacaggt gctgaaagcg aggctttttg gcctctgtcg
     tttcctttct ctgtttttgt ccgtggaatg aacaatggaa gtcaaccagc ttgtattcta
     tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     ggtaacgccg ttccgcaccc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     ccagatcccc gggcgagctc gaatt
//