Return to this vector's summary.
ID LORDEL preliminary; circular DNA; SYN; 5065 BP.
XX
AC IG9926;
XX
DT 01-JUL-1995 (Rel. 11, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli cosmid vector Lordel - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pUCSP from pUC12 & pSP64, Sp6 promoter
RC pUCT7 from pUC12 & pT7-2, T7 promoter
RC Lorus from Loric & pUCSP
RC Lordel from Lorus
RC LordelO from Lordel
RC LordelB from LordelO
RC LdBp12 from LordelB & pUC12
RC Loris from LdBp12
RC pUCT7Bdelta from pUCT7
RC LorspT7 from Loris & pUCT7Bdelta
RC LoristB from LorspT7
RA Cross S.H., Little P.F.;
RT "A cosmid vector for systematic chromosome walking";
RL Gene 49:9-22(1986).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NM (Lordel)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (Loric)(pSP64)(pUC12)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC12 remove BamHI-HindIII 27bp 249..276,
FT \ MCS/2653bp
FT 2. pSP64 Sau3AI/MboI-HindIII 256bp 2751..2999..8,
FT \ Sp6 promoter
FT -> pUCSP 2909bp
FT 1. Loric ClaI-HindIII 6192bp 300..6492
FT Klenow:
FT 2. pUCSP EcoRI-HindIII 274bp 231..249 - 276,
FT \ Sp6 promoter
FT \ pUC12 231..276
FT \ pSP64 ..8
FT Klenow:
FT -> Lorus 6466bp
FT 1. Lorus remove SspI-SstII/SacII 1411bp,
FT \ Loric 1279..2690/5055bp
FT T4 DNA polymerase
FT -> Lordel 5055bp [5193bp]"
FT - 1..979
FT /note="Loric 300..1278 979bp
FT SspI = AAT^ATT
FT SacII = CC GC^GG"
FT - 980..4787
FT /note="Loric 2684..6491 3808bp
FT HindIII = A^AGCTT"
FT - 4788..5047
FT /note="pSP64 2751..2999..11 260bp complement
FT MboI = Sau3AI = ^GATC
FT BamHI = G^GATCC"
FT - 5048..5065
FT /note="pUC12 231..248 18bp complement
FT EcoRI = G^AATT C
FT ClaI = AT^CGAT"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 5065 BP; 1152 A; 1310 C; 1287 G; 1313 T; 3 other;
cgataaatgg cttcagaaca ggcattcgcg tctgaatatc ctttggttcc cataccgtat
aaccatttgg ctgtccaagc tccgggttga tatcaacctg caatacggtg agcggtatat
cccagaactt cacaacttcc ctgacaaacc gatatgtcat tggatgttca caacctgtat
ccatgaaaac gtaatgcacg tctttacctg cccgtcgctt ttgctccatt agccagagca
aatatgctga cgtcctgcca ccggagaaac taacgacatt tatcatgcag ccctgtctcc
ccatctcgct ttccactcca gagccagtct cgcttcgtct gaccacttaa cgccacgctc
tgtaccgaat gcctgtataa gctctaatag ctccgcaaat tcgcctacac gcatcctgct
ggttgactgg cctattacca caaagccatt cccggcaagg ttaggaacaa catcctgctg
ctttaatgct gcggtaaaca cacacttcca gctttctgca tccagccagc gaccatgcca
ttcaacctga cgagagacgt cacctaagca ggcccatagc ttcctgtttt ggtctaagct
gcggttgcgt tcctgaatgg ttactacgat tggtttggtt gggtctggaa ggatttgctg
tactgcgtga atagcgtttt gctgatgtgc tggagatcga atttcaaagg ttagtttttt
catgacttcc ctctccccca aataaaaagg cctgcgatta ccagcaggcc tgttattagc
tcagtaatgt agatggtcat cttttaactc catataccgc caatacccgt ttcatcgcgg
cactctggcg acactcctta aaaaccaggt tcgtgctcat ctttccttcc cgttcttccc
tggtagcaaa ccggtaatac accgttcgcc agaccttacc ttcgataacc agaagacctg
cccgtgccat tttagccgcg ataatattca ttctgacgag ttctaacttg gcttcattgc
accgtttgac aggtaacttt gtaatctcgc taagttgaga atcggtgatt ctgtccattg
gtttattcca cccataggtt ttacgcagaa tggcaagcag cactttaaac tgtcgcttgg
tcagatctgc gcccgaataa gcctcaagca gcatatttga tagtctggcg taaccatcat
cgagatctgc cacattacgc tcctgtccgg caaagttacc tctgccgaag ttgagtattt
ttgctgtatt tgtcataatg actcctgttg atagatccag taatgacctc agaactccat
ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatcgcagc
aacttgtcgc gccaatcgag ccatgtcgtc gtcaacgacc ccccattcaa gaacagcaag
cagcattgag aactttggaa tccagtccct cttccacctg ctgatctgcg acttatcaac
gcccacagct tccgctgtct tctcagttcc aagcattgcg attttgttaa gcaacgcact
ctcgattcgt agagcctcgt tgcgtttgtt tgcacgaacc atatgtaagt atttccttag
ataacaattg attgaatgta tgcaaataaa tgcatacacc ataggtgtgg tttaatttga
tgcccttttt cagggctgga atgtgtaaga gcggggttat ttatgctgtt gtttttttgt
tactcgggaa gggctttacc tcttccgcat aaacgcttcc atcagcgttt atagttaaaa
aaatctttcg gcctgcatga atggccttgt tgatcgcgct ttgatatacg ccgagatctt
tagctgtctt ggtttgccca aagcgcattg cataatcttt cagggttatg cgttgttcca
tacaacctcc ttagtacatg caaccattat caccgccaga ggtaaaatag tcaacacgca
cggtgttaga tatttatccc ttgcggtgat agatttaacg tatgagcaca aaaaagaaac
cattaacaca agagcagctt gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga
aaaatgaact tggcttatcc caggaatctg tcgcagacaa gatggggatg gggcagtcag
gcgttggtgc tttatttaat ggcatcaatg cattaaatgc ttataacgcc gcattgcttg
caaaaattct caaagttagc gttgaagaat ttagcccttc aatcgccaga gaaatctacg
agatgtatga agcggttagt atgcagccgt cacttagaag tgagtatgag taccctgttt
tttctcatgt tcaggcaggg atgttctcac ctgagcttag aacctttacc aaaggtgatg
cggagagatg ggtaagcaca accaaaaaag ccagtgattc tgcattctgg cttgaggttg
aaggtaattc catgaccgca ccaacaggct ccaagccaag ctagcttcac gctgccgcaa
gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa
acggtgctga ccccggatga atgtcactac tgggctatct ggacaaggga aaacgcaagc
gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
ttatggacag caagcgaacc ggaattgcca gctggggcgc catctggtaa ggttgggaag
ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgagcagcg cggctatcgt
ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
gggactggct gctattgggc gaagtgccgg ggcaggatct ccagtcatct caccttgctc
ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggctgg
aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgcgatt tcgattccac
cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
ggagtgggga ggcacgatgg ccgctttggt cgaccggacg ggacgctcct gcgcctgata
cagaacgaat tgcttgcagg catctcatga gtgtgtcttc ccgttttccg cctgaggtca
ctgcgtggat ggagcgctgg cgcctgctgc gcgacggcga gctgctcacc acccactcga
gctggatact tcgcgtccgc cagggggaca tgccggcgat gctgaaggtc gcccgcattc
ccgatgaaga ggccggttac cgcctgttga cctggtggga cgggcagggc gccccccgac
tcttcgcctc ggcggcgggc gctctgctca tggagcgcgc gtccggggcc ggggaccttg
cacagatagc gtggtccggc caggacgacg aggcttgcag gatcaattcn ggatccnntt
gacatgaggt tgccccgtat tcagtgtcgc tgatttgtat tgtctgaagt tgtttttacg
ttaagttgat gcagatcaat taatacgata cctgcgtcat aattgattat ttgacgtggt
ttgatggcct ccacgcacgt tgtgatatgt agatgataat cattatcact ttacgggtcc
tttccggtga tccgacaggt tacggggcgg cgacctcgcg ggttttcgct atttatgaaa
attttccggt ttaaggcgtt tccgttcttc ttcgtcataa cttaatgttt ttatttaaaa
taccctctga aaagaaagga aacgacaggt gctgaaagcg aggctttttg gcctctgtcg
tttcctttct ctgtttttgt ccgtggaatg aacaatggaa gtcaaccagc ttgtattcta
tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
ggtaacgccg ttccgcaccc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
ccagatcccc gggcgagctc gaatt
//