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ID   LORIC      preliminary; circular DNA; SYN; 6604 BP.
XX
AC   IG0003; ATCC37370;
XX
DT   20-NOV-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli cosmid vector Loric - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pPZ74 from pBR325 & pIJ303]
RC   [pBRNeo from pBR322 & pRZ112, neo gene]
RC   lambda NEO from lambda & pBRNeo
RC   plasmid from lambda NEO
RC   PiCos from pideltaplac & MUA-10
RC   Loric from PiCos & plasmid
RA   Little P.F., Cross S.H.;
RT   "A cosmid vector that facilitates restriction enzyme mapping";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:3159-3163(1985).
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RN   [2]
RC   pRZ121, pRZ123, pRZ124, pRZ129 from pRZ102
RC   pRZ141, pRZ143 from pRZ102
RC   pRZ149, pRZ154 from pRZ143 & pRZ112
RC   pRZ152 from pRZ112 & pRZ102
RC   pRZ172, pRZ174 from pRZ112 & pRZ102
RC   pRZ161, pRZ164, pRZ165, pRZ166 from pRZ102
RA   Rothstein S.J., Jorgensen R.A., Postle K., Reznikoff W.S.;
RT   "The inverted repeats of Tn5 are functionally different";
RL   Cell 19:795-805(1980).
XX
RN   [3]
RC   piBP from piVX & human beta-globin gene
RC   piBdeltaP from piBP
RC   piBPSV from piBdeltaP & pPO
RC   piSVHP, piSVHS from piBPSV
RC   piSVHPBP from pSV0d & piBdeltaP
RC   piSVHPdeltaBP from piSVHP & SV40
RC   piSV from piSVHPBP
RC   piSVHPplac, pSVHSplac from SV40 ori/enhancer & piSV & piVX
RC   lambda HbetaG1 from Charon 4A & human beta-globin gene
RC   piSVbeta from lambda HbetaG1 & piSVHPBP, human beta-globin gene
RC   [pideltaplac from lacZ gene & human beta-globin gene]
RA   Little P.F., Treisman R., Bierut L., Seed B., Maniatis T.;
RT   "Plasmid vectors for the rapid isolation and transcriptional
RT   analysis of human beta-globin gene alleles";
RL   Mol. Biol. Med. 1:473-488(1983).
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RN   [4]
RC   pPO from pBR322 & SV40 ori
RA   Hanahan D.;
RT   ;
RL   Unpublished (1983).
XX
CC   ATCC size is 6270 bp.
CC   Molecule is derived from the bacteriophage lambda origin of
CC   replication (oriC) and may be more stable than certain cosmids.
CC   Recombinants can be efficiently packaged to yield 100-300 ug
CC   DNA/liter.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (Loric)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(E.coli)
CC   CP ()
CC   FN (mapping site)
CC   SE ()
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   transposition
FT                   2. Tn5 5818bp
FT                   -> pRZ102 10179bp
FT                   1. pRZ102 remove pBR322 sequences
FT                   -> pRZ112
FT                   1. pRZ112 HincII-HincII 2499bp 188..2687, neo gene
FT                   BamHI linker 10bp nnggatccnn:BamHI linker 10bp
FT                   \ nnggatccnn
FT                   HindIII-BamHI 1493bp 1196..2689, neo/kan gene
FT                   2. pBR322 remove HindIII-BamHI 346bp 30..376, 4015bp
FT                   -> pBR-neo 5508bp
FT                   1. lambda remove HindIII-BamHI 4272bp 37460..41732,
FT                   \ 44230bp
FT                   \ HindIII 23131 25158 27480 36896 37460 44142;
FT                   \ BamHI 5506 22347 27973 34500 41733
FT                   2. pBRNeo HindIII-BamHI 1493bp 1196..2689, neo/kan
FT                   -> lambda NEO 44000bp
FT                   1. E. coli, lacZ gene
FT                   2. human beta-globin gene
FT                   -> pideltaplac
FT                   1. pideltaplac PstI
FT                   2. MUA-10 PstI-PstI 403bp, lambda cos
FT                   \ lambda HindII-HindII 403bp 48299..48502..200, cos
FT                   -> PiCos
FT                   1. PiCos EcoRI-EcoRI 630bp, lambda cos
FT                   Klenow:Klenow
FT                   2. lambda NEO HindIII-BamHI 1493bp 1196..2689, neo/kan
FT                   Klenow:Klenow
FT                   -> Loric 6604bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-XbaI-HindIII-ClaI-SacII"
FT   CDS             4631..5426
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan)"
FT   rep_origin      6155..6557
FT                   /note="ORI bacteriophage lambda cos region"
XX
SQ   Sequence 6604 BP; 1447 A; 1716 C; 1665 G; 1769 T; 7 other;
     cgttgatttt ttaatgcaga atatgcagtt accgagatgt tccggtattt gcaaatcgaa
     tggttgttgc ttccaccatg cgaggatatc ttccttctca aagtctgaca gttcagcaag
     atatctgatt ccaggctttg gctttagccg cttcggttca tcagctctga tgccaatcca
     cgtggtgtaa ttccctcgcc cgaaatggtc atcacagtat ttggtgaagg gaacgagttt
     taatctgtca gtgcagaacg cgccgccgac gtatggagtg ccatatttct ttaccatatc
     gataaatggc ttcagaacag gcattcgcgt ctgaatatcc tttggttccc ataccgtata
     accatttggc tgtccaagct ccgggttgat atcaacctgc aatacggtga gcggtatatc
     ccagaacttc acaacttccc tgacaaaccg atatgtcatt ggatgttcac aacctgtatc
     catgaaaacg taatgcacgt ctttacctgc ccgtcgcttt tgctccatta gccagagcaa
     atatgctgac gtcctgccac cggagaaact aacgacattt atcatgcagc cctgtctccc
     catctcgctt tccactccag agccagtctc gcttcgtctg accacttaac gccacgctct
     gtaccgaatg cctgtataag ctctaatagc tccgcaaatt cgcctacacg catcctgctg
     gttgactggc ctattaccac aaagccattc ccggcaaggt taggaacaac atcctgctgc
     tttaatgctg cggtaaacac acacttccag ctttctgcat ccagccagcg accatgccat
     tcaacctgac gagagacgtc acctaagcag gcccatagct tcctgttttg gtctaagctg
     cggttgcgtt cctgaatggt tactacgatt ggtttggttg ggtctggaag gatttgctgt
     actgcgtgaa tagcgttttg ctgatgtgct ggagatcgaa tttcaaaggt tagttttttc
     atgacttccc tctcccccaa ataaaaaggc ctgcgattac cagcaggcct gttattagct
     cagtaatgta gatggtcatc ttttaactcc atataccgcc aatacccgtt tcatcgcggc
     actctggcga cactccttaa aaaccaggtt cgtgctcatc tttccttccc gttcttccct
     ggtagcaaac cggtaataca ccgttcgcca gaccttacct tcgataacca gaagacctgc
     ccgtgccatt ttagccgcgg cctgatttat gctggttact gttgcgcctg ttagcgcggc
     aacgtccggc gcacagaagc tattatgcgt ccccaggtaa tgaataattg cctctttgcc
     cgtcatacac ttgctccttt cagtccgaac ttagctttga tttctgcgat cttcgccaga
     gcctgtgcac gatttagagg tctaccgccc atgacaggaa gttgttttac tggttcaggg
     atcgcctcac cacggttaat tctcgcagtc atatggacaa gctcatctgc ggccttacgg
     cgtaattccg catcagtaag cgcattggcc cgcatgttct gatacaggtt ggtaaccagc
     cagtagtgcg cgtttgattt ccacggataa gactccgcat ccggatacag gcctcgcttc
     cggcaatact cgtaaaccat atcaaccagc tcgctgacgt ttggcagtcc ggcggtaacg
     gatgcttctt cccggcacca tgcaacaaac tgcccgggtg atggcagaaa tggtcgattc
     tgccgacggg ctacgcgcat tcctgcgtta acctgttcca tcgtggtgat cccgttttcc
     cgaaaagcca gaacccactg gcgacggatt tcgttcactt cgttctggtc acggttagcc
     aggctcgccg ggaaagttgc cagtaactgg ctgaacacac cgttgatgat ctgcgctacc
     tgctgtacct gcggcttttc gtcgtactgt tccggcatgt tgttggcgat ccgacgcatc
     tgctcacggt caaagttaac catctgtgcg gcgatgtttt tcatagatcc accccgtaaa
     tccagtctgt gtttgtcagg tcgagttttg gtttgctggc tgtcacgcct gcctgttgct
     tgttacggtt gatttcgagt tgggtccact tatcgcggag tttggccggg ctcagcacgt
     taccggacca gaagttgtcc tggcatgccc agcggaacag cacacacatg tcgcggtggt
     tacgtccgtc acgttcacgc atcaggcgga tatcgttagc ccacccagca aaattcggtt
     ttctggctga tggtgcgata gtcttcacca tgtcaaacat ccactctgcg gcggtcaggt
     cttctgctgt cccccacttg ctgccgctct gaattgcagc atccggtttc accacagaaa
     ggtcgttttc tggctggtca gaggattcgc cagaattctc tgacgaataa tcttttcttt
     tttcttttgt aatagtgtct tttgtgtccc cctgttttga gggatagcaa tcccccaatt
     tgagggatgt tttatccctc gttttagggg attttccctc gttttgaggg atgcaccatt
     ctgagatgtt tttatttggt ccaaacatgc cgccttgctg cttgataata ttcattctga
     cgagttctaa cttggcttca ttgcaccgtt tgacaggtaa ctttgtaatc tcgctaagtt
     gagaatcggt gattctgtcc attggtttat tccacccata ggttttacgc agaatggcaa
     gcagcacttt aaactgtcgc ttggtcagat ctgcgcccga ataagcctca agcagcatat
     ttgatagtct ggcgtaacca tcatcgagat ctgccacatt acgctcctgt ccggcaaagt
     tacctctgcc gaagttgagt atttttgctg tatttgtcat aatgactcct gttgatagat
     ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     ttttttattg gtgagaatcg cagcaacttg tcgcgccaat cgagccatgt cgtcgtcaac
     gaccccccat tcaagaacag caagcagcat tgagaacttt ggaatccagt ccctcttcca
     cctgctgatc tgcgacttat caacgcccac agcttccgct gtcttctcag ttccaagcat
     tgcgattttg ttaagcaacg cactctcgat tcgtagagcc tcgttgcgtt tgtttgcacg
     aaccatatgt aagtatttcc ttagataaca attgattgaa tgtatgcaaa taaatgcata
     caccataggt gtggtttaat ttgatgccct ttttcagggc tggaatgtgt aagagcgggg
     ttatttatgc tgttgttttt ttgttactcg ggaagggctt tacctcttcc gcataaacgc
     ttccatcagc gtttatagtt aaaaaaatct ttcggcctgc atgaatggcc ttgttgatcg
     cgctttgata tacgccgaga tctttagctg tcttggtttg cccaaagcgc attgcataat
     ctttcagggt tatgcgttgt tccatacaac ctccttagta catgcaacca ttatcaccgc
     cagaggtaaa atagtcaaca cgcacggtgt tagatattta tcccttgcgg tgatagattt
     aacgtatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc
     ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag
     acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa
     atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc
     cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta
     gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc
     ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg
     attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc
     caagctagct tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa
     cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca ctactgggct
     atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     gcgccatctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc
     atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     caggacgagc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     atctccagtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     tcgagcgagc acgtactcgg ctggaagccg gtcttgtcga tcaggatgat ctggacgaag
     agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     gccatcacgc gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     ccaccccggg ctcgatcccc tcgcgagttg gttcagctgc tgcctgaggc tggacgacct
     cgcggagttc taccggcagt gcaaatccgt cggcatccag gaaaccagca gcggctatcc
     gcgcatccat gcccccgaac tgcaggagtg gggaggcacg atggccgctt tggtcgaccg
     gacgggacgc tcctgcgcct gatacagaac gaattgcttg caggcatctc atgagtgtgt
     cttcccgttt tccgcctgag gtcactgcgt ggatggagcg ctggcgcctg ctgcgcgacg
     gcgagctgct caccacccac tcgagctgga tacttcgcgt ccgccagggg gacatgccgg
     cgatgctgaa ggtcgcccgc attcccgatg aagaggccgg ttaccgcctg ttgacctggt
     gggacgggca gggcgccccc cgactcttcg cctcggcggc gggcgctctg ctcatggagc
     gcgcgtccgg ggccggggac cttgcacaga tagcgtggtc cggccaggac gacgaggctt
     gcaggatcaa ttcnggatcc nnttgacatg aggttgcccc gtattcagtg tcgctgattt
     gtattgtctg aagttgtttt tacgttaagt tgatgcagat caattaatac gatacctgcg
     tcataattga ttatttgacg tggtttgatg gcctccacgc acgttgtgat atgtagatga
     taatcattat cactttacgg gtcctttccg gtgatccgac aggttacggg gcggcgacct
     cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc
     ataacttaat gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa
     agcgaggctt tttggcctct gtcgtttcct ttctctgttt ttgtccgtgg aatgaacaat
     ggaagtcaac aannntctag anaagcttac tcattaggca ccccaggctt tacactttat
     gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     attc
//