back Return to this vector's summary.
ID   LORISTB    preliminary; circular DNA; SYN; 5614 BP.
XX
AC   M15423;
XX
DT   16-JUL-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector LoristB - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5614
RC   pUCSP from pUC12 & pSP64, Sp6 promoter
RC   pUCT7 from pUC12 & pT7-2, T7 promoter
RC   Lorus from Loric & pUCSP
RC   Lordel from Lorus
RC   LordelO from Lordel
RC   LordelB from LordelO
RC   LdBp12 from LordelB & pUC12
RC   Loris from LdBp12
RC   pUCT7Bdelta from pUCT7
RC   LorspT7 from Loris & pUCT7Bdelta
RC   LoristB from LorspT7
RA   Cross S.H., Little P.F.;
RT   "A cosmid vector for systematic chromosome walking";
RL   Gene 49:9-22(1986).
XX
CC   NM (LoristB)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (chromosome walking)
CC   SE ()
CC   PA (lambda)(Sp6)(T7)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 remove BamHI-HindIII 27bp 249..276,
FT                   \ MCS/2653bp
FT                   2. pSP64 HindIII-Sau3AI/MboI 256bp 2751..2999..8,
FT                   \ Sp6 promoter
FT                   -> pUCSP 2909bp
FT                   1. Loric ClaI-HindIII 6192bp 300..6492
FT                   :Klenow
FT                   2. pUCSP HindIII-EcoRI 274bp 231..249 - 276,
FT                   \ Sp6 promoter
FT                   :Klenow
FT                   -> Lorus 6466bp
FT                   1. Lorus remove SspI-SstII/SacII 1411bp,
FT                   \ Loric 1279..2690/5193bp
FT                   T4 DNA polymerase
FT                   -> Lordel 5193bp
FT                   1. Lordel BamHI 5193bp, Loric 6076..6076
FT                   Klenow
FT                   -> LordelO 5193bp [ClaI]
FT                   1. LordelO HindIII-HindIII 5181bp,
FT                   \ Loric 6504..6604..6492
FT                   Klenow
FT                   EcoRI 5181bp, Loric 2494..2494
FT                   BamHI linker 6bp ggatcc
FT                   -> LordelB 5187bp
FT                   1. LordelB XbaI 5187bp, Loric 6497..6497
FT                   Klenow
FT                   2. pUC12 EcoRI 2680bp 231..231
FT                   Klenow
FT                   -> LdBp12 7867bp
FT                   1. LdBp12 remove HindIII-HindIII, pUC12 ori
FT                   \ pUC12 276..276
FT                   -> Loris 7800bp [SstI]
FT                   1. pUC12 remove HindIII-HaeII 168bp 276..444,
FT                   \ MCS/2512bp
FT                   2. pT7-2 HindIII-HaeII 202bp 239..441, T7 promoter
FT                   -> pUCT7 2714bp
FT                   1. pUCT7 BamHI 2714bp 249..249
FT                   Klenow
FT                   -> pUCT7Bdelta 2714bp
FT                   1. Loris HindIII 7800bp, pUC12 276..276
FT                   2. pUCT7Bdelta HindIII 2714bp 276..276
FT                   -> LorspT7 10500bp
FT                   1. LorspT7 remove SstI/SacI-SstI/SacI,
FT                   \ pUC12 ori/pUC12 241
FT                   -> LoristB 5614bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos region"
XX
SQ   Sequence 5614 BP; 1279 A; 1460 C; 1440 G; 1435 T; 0 other;
     atcgatccgg gcaacgttgt tgccattgct gcaggggggg gggggggggg gggggggggt
     tgacttccat tgttcattcc acggacaaaa acagagaaag gaaacgacag aggccaaaaa
     gcctcgcttt cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat
     taagttatga cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa
     aacccgcgag gtcgccgccc gtaacctgtc ggatcaccgg aaaggacccg taaagtgata
     atgattatca tctacatatc acaacgtgcg tggaggccat caaaccacgt caaataatca
     attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa acaacttcag
     acaatacaaa tcagcgacac tgaatacggg gcaacctcat gtcaaccccc cccccccccc
     cccccccccc ctgcaggcgg agaactggta ggtatggaag atctctagag aattcgagct
     cgcccgggga tcgatcctct agagtcctga tgcggtattt tctccttacg catctgtgcg
     gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     gccagtatat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     gcgaggccca gctggcttat cgaaattaat acgactcact atagggagac cggaagctta
     ggatcctatg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
     attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct
     tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac
     gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca
     gcagggtcat cgctagccag atccccgggc gagctgcggc ctgatttatg ctggttactg
     ttgcgcctgt tagcgcggca acgtccggcg cacagaagct attatgcgtc cccaggtaat
     gaataattgc ctctttgccc gtcatacact tgctcctttc agtccgaact tagctttgat
     ttctgcgatc ttcgccagag cctgtgcacg atttagaggt ctaccgccca tgacaggaag
     ttgttttact ggttcaggga tcgcctcacc acggttaatt ctcgcagtca tatggacaag
     ctcatctgcg gccttacggc gtaattccgc atcagtaagc gcattggccc gcatgttctg
     atacaggttg gtaaccagcc agtagtgcgc gtttgatttc cacggataag actccgcatc
     cggatacagg cctcgcttcc ggcaatactc gtaaaccata tcaaccagct cgctgacgtt
     tggcagtccg gcggtaacgg atgcttcttc ccggcaccat gcaacaaact gcccgggtga
     tggcagaaat ggtcgattct gccgacgggc tacgcgcatt cctgcgttaa cctgttccat
     cgtggtgatc ccgttttccc gaaaagccag aacccactgg cgacggattt cgttcacttc
     gttctggtca cggttagcca ggctcgccgg gaaagttgcc agtaactggc tgaacacacc
     gttgatgatc tgcgctacct gctgtacctg cggcttttcg tcgtactgtt ccggcatgtt
     gttggcgatc cgacgcatct gctcacggtc aaagttaacc atctgtgcgg cgatgttttt
     catagatcca ccccgtaaat ccagtctgtg tttgtcaggt cgagttttgg tttgctggct
     gtcacgcctg cctgttgctt gttacggttg atttcgagtt gggtccactt atcgcggagt
     ttggccgggc tcagcacgtt accggaccag aagttgtcct ggcatgccca gcggaacagc
     acacacatgt cgcggtggtt acgtccgtca cgttcacgca tcaggcggat atcgttagcc
     cacccagcaa aattcggttt tctggctgat ggtgcgatag tcttcaccat gtcaaacatc
     cactctgcgg cggtcaggtc ttctgctgtc ccccacttgc tgccgctctg aattgcagca
     tccggtttca ccacagaaag gtcgttttct ggctggtcag aggattcgcc agaattctct
     gacgaataat cttttctttt ttcttttgta atagtgtctt ttgtgtcccc ctgttttgag
     ggatagcaat cccccaattt gagggatgtt ttatccctcg ttttagggga ttttccctcg
     ttttgaggga tgcaccattc tgagatgttt ttatttggtc caaacatgcc gccttgctgc
     ttgataatat tcattctgac gagttctaac ttggcttcat tgcaccgttt gacaggtaac
     tttgtaatct cgctaagttg agaatcggtg attctgtcca ttggtttatt ccacccatag
     gttttacgca gaatggcaag cagcacttta aactgtcgct tggtcagatc tgcgcccgaa
     taagcctcaa gcagcatatt tgatagtctg gcgtaaccat catcgagatc tgccacatta
     cgctcctgtc cggcaaagtt acctctgccg aagttgagta tttttgctgt atttgtcata
     atgactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     gctcggttgc cgccgggcgt tttttattgg tgagaatcgc agcaacttgt cgcgccaatc
     gagccatgtc gtcgtcaacg accccccatt caagaacagc aagcagcatt gagaactttg
     gaatccagtc cctcttccac ctgctgatct gcgacttatc aacgcccaca gcttccgctg
     tcttctcagt tccaagcatt gcgattttgt taagcaacgc actctcgatt cgtagagcct
     cgttgcgttt gtttgcacga accatatgta agtatttcct tagataacaa ttgattgaat
     gtatgcaaat aaatgcatac accataggtg tggtttaatt tgatgccctt tttcagggct
     ggaatgtgta agagcggggt tatttatgct gttgtttttt tgttactcgg gaagggcttt
     acctcttccg cataaacgct tccatcagcg tttatagtta aaaaaatctt tcggcctgca
     tgaatggcct tgttgatcgc gctttgatat acgccgagat ctttagctgt cttggtttgc
     ccaaagcgca ttgcataatc tttcagggtt atgcgttgtt ccatacaacc tccttagtac
     atgcaaccat tatcaccgcc agaggtaaaa tagtcaacac gcacggtgtt agatatttat
     cccttgcggt gatagattta acgtatgagc acaaaaaaga aaccattaac acaagagcag
     cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta
     tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt
     aatggcatca atgcattaaa tgcttataac gccgcattgc ttacaaaaat tctcaaagtt
     agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt
     agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca
     gggatgttct cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc
     acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc
     gcaccaacag gctccaagcc aagctagctt cacgctgccg caagcactca gggcgcaagg
     gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
     caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     tggcgaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     ctcatgctgg agttcttcgc ccaccccggg ctcgatcccc tcgcgagttg gttcagctgc
     tgcctgaggc tggacgacct cgcggagttc taccggcagt gcaaatccgt cggcatccag
     gaaaccagca gcggctatcc gcgcatccat gcccccgaac tgcaggagtg gggaggcacg
     atggccgctt tggtcggatc aattcgcgcg accg
//