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ID   LORUS      preliminary; circular DNA; SYN; 6470 BP.
XX
AC   IG9925;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector Lorus - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUCSP from pUC12 & pSP64, Sp6 promoter
RC   pUCT7 from pUC12 & pT7-2, T7 promoter
RC   Lorus from Loric & pUCSP
RC   Lordel from Lorus
RC   LordelO from Lordel
RC   LordelB from LordelO
RC   LdBp12 from LordelB & pUC12
RC   Loris from LdBp12
RC   pUCT7Bdelta from pUCT7
RC   LorspT7 from Loris & pUCT7Bdelta
RC   LoristB from LorspT7
RA   Cross S.H., Little P.F.;
RT   "A cosmid vector for systematic chromosome walking";
RL   Gene 49:9-22(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (Lorus)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Loric)(pSP64)(pUC12)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 remove BamHI-HindIII 27bp 249..276,
FT                   \ MCS/2653bp
FT                   2. pSP64 Sau3AI/MboI-HindIII 256bp 2751..2999..8,
FT                   \ Sp6 promoter
FT                   -> pUCSP 2909bp
FT                   1. Loric ClaI-HindIII 6192bp 300..6492
FT                   Klenow:
FT                   2. pUCSP EcoRI-HindIII 274bp 231..249 - 276,
FT                   \ Sp6 promoter
FT                   \ pUC12 231..276
FT                   \ pSP64 ..8
FT                   Klenow:
FT                   -> Lorus 6466bp"
FT   -               1..6192
FT                   /note="Loric 300..6491 6192bp
FT                   HindIII = A^AGCTT"
FT   -               6193..6452
FT                   /note="pSP64 2751..2999..11 260bp complement
FT                   MboI = Sau3AI = ^GATC
FT                   BamHI =        G^GATCC"
FT   -               6453..6470
FT                   /note="pUC12 231..248 18bp complement
FT                   EcoRI = G^AATT C
FT                   ClaI =      AT^CGAT"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6470 BP; 1417 A; 1691 C; 1638 G; 1721 T; 3 other;
     cgataaatgg cttcagaaca ggcattcgcg tctgaatatc ctttggttcc cataccgtat
     aaccatttgg ctgtccaagc tccgggttga tatcaacctg caatacggtg agcggtatat
     cccagaactt cacaacttcc ctgacaaacc gatatgtcat tggatgttca caacctgtat
     ccatgaaaac gtaatgcacg tctttacctg cccgtcgctt ttgctccatt agccagagca
     aatatgctga cgtcctgcca ccggagaaac taacgacatt tatcatgcag ccctgtctcc
     ccatctcgct ttccactcca gagccagtct cgcttcgtct gaccacttaa cgccacgctc
     tgtaccgaat gcctgtataa gctctaatag ctccgcaaat tcgcctacac gcatcctgct
     ggttgactgg cctattacca caaagccatt cccggcaagg ttaggaacaa catcctgctg
     ctttaatgct gcggtaaaca cacacttcca gctttctgca tccagccagc gaccatgcca
     ttcaacctga cgagagacgt cacctaagca ggcccatagc ttcctgtttt ggtctaagct
     gcggttgcgt tcctgaatgg ttactacgat tggtttggtt gggtctggaa ggatttgctg
     tactgcgtga atagcgtttt gctgatgtgc tggagatcga atttcaaagg ttagtttttt
     catgacttcc ctctccccca aataaaaagg cctgcgatta ccagcaggcc tgttattagc
     tcagtaatgt agatggtcat cttttaactc catataccgc caatacccgt ttcatcgcgg
     cactctggcg acactcctta aaaaccaggt tcgtgctcat ctttccttcc cgttcttccc
     tggtagcaaa ccggtaatac accgttcgcc agaccttacc ttcgataacc agaagacctg
     cccgtgccat tttagccgcg gcctgattta tgctggttac tgttgcgcct gttagcgcgg
     caacgtccgg cgcacagaag ctattatgcg tccccaggta atgaataatt gcctctttgc
     ccgtcataca cttgctcctt tcagtccgaa cttagctttg atttctgcga tcttcgccag
     agcctgtgca cgatttagag gtctaccgcc catgacagga agttgtttta ctggttcagg
     gatcgcctca ccacggttaa ttctcgcagt catatggaca agctcatctg cggccttacg
     gcgtaattcc gcatcagtaa gcgcattggc ccgcatgttc tgatacaggt tggtaaccag
     ccagtagtgc gcgtttgatt tccacggata agactccgca tccggataca ggcctcgctt
     ccggcaatac tcgtaaacca tatcaaccag ctcgctgacg tttggcagtc cggcggtaac
     ggatgcttct tcccggcacc atgcaacaaa ctgcccgggt gatggcagaa atggtcgatt
     ctgccgacgg gctacgcgca ttcctgcgtt aacctgttcc atcgtggtga tcccgttttc
     ccgaaaagcc agaacccact ggcgacggat ttcgttcact tcgttctggt cacggttagc
     caggctcgcc gggaaagttg ccagtaactg gctgaacaca ccgttgatga tctgcgctac
     ctgctgtacc tgcggctttt cgtcgtactg ttccggcatg ttgttggcga tccgacgcat
     ctgctcacgg tcaaagttaa ccatctgtgc ggcgatgttt ttcatagatc caccccgtaa
     atccagtctg tgtttgtcag gtcgagtttt ggtttgctgg ctgtcacgcc tgcctgttgc
     ttgttacggt tgatttcgag ttgggtccac ttatcgcgga gtttggccgg gctcagcacg
     ttaccggacc agaagttgtc ctggcatgcc cagcggaaca gcacacacat gtcgcggtgg
     ttacgtccgt cacgttcacg catcaggcgg atatcgttag cccacccagc aaaattcggt
     tttctggctg atggtgcgat agtcttcacc atgtcaaaca tccactctgc ggcggtcagg
     tcttctgctg tcccccactt gctgccgctc tgaattgcag catccggttt caccacagaa
     aggtcgtttt ctggctggtc agaggattcg ccagaattct ctgacgaata atcttttctt
     ttttcttttg taatagtgtc ttttgtgtcc ccctgttttg agggatagca atcccccaat
     ttgagggatg ttttatccct cgttttaggg gattttccct cgttttgagg gatgcaccat
     tctgagatgt ttttatttgg tccaaacatg ccgccttgct gcttgataat attcattctg
     acgagttcta acttggcttc attgcaccgt ttgacaggta actttgtaat ctcgctaagt
     tgagaatcgg tgattctgtc cattggttta ttccacccat aggttttacg cagaatggca
     agcagcactt taaactgtcg cttggtcaga tctgcgcccg aataagcctc aagcagcata
     tttgatagtc tggcgtaacc atcatcgaga tctgccacat tacgctcctg tccggcaaag
     ttacctctgc cgaagttgag tatttttgct gtatttgtca taatgactcc tgttgataga
     tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
     gttttttatt ggtgagaatc gcagcaactt gtcgcgccaa tcgagccatg tcgtcgtcaa
     cgacccccca ttcaagaaca gcaagcagca ttgagaactt tggaatccag tccctcttcc
     acctgctgat ctgcgactta tcaacgccca cagcttccgc tgtcttctca gttccaagca
     ttgcgatttt gttaagcaac gcactctcga ttcgtagagc ctcgttgcgt ttgtttgcac
     gaaccatatg taagtatttc cttagataac aattgattga atgtatgcaa ataaatgcat
     acaccatagg tgtggtttaa tttgatgccc tttttcaggg ctggaatgtg taagagcggg
     gttatttatg ctgttgtttt tttgttactc gggaagggct ttacctcttc cgcataaacg
     cttccatcag cgtttatagt taaaaaaatc tttcggcctg catgaatggc cttgttgatc
     gcgctttgat atacgccgag atctttagct gtcttggttt gcccaaagcg cattgcataa
     tctttcaggg ttatgcgttg ttccatacaa cctccttagt acatgcaacc attatcaccg
     ccagaggtaa aatagtcaac acgcacggtg ttagatattt atcccttgcg gtgatagatt
     taacgtatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc
     cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca
     gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta
     aatgcttata acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc
     ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt
     agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag
     cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt
     gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag
     ccaagctagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga
     acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc actactgggc
     tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     ggcgccatct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
     catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     gcaggacgag cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     gatctccagt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     atcgagcgag cacgtactcg gctggaagcc ggtcttgtcg atcaggatga tctggacgaa
     gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     tgccatcacg cgatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     cccaccccgg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc
     tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc
     cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc
     ggacgggacg ctcctgcgcc tgatacagaa cgaattgctt gcaggcatct catgagtgtg
     tcttcccgtt ttccgcctga ggtcactgcg tggatggagc gctggcgcct gctgcgcgac
     ggcgagctgc tcaccaccca ctcgagctgg atacttcgcg tccgccaggg ggacatgccg
     gcgatgctga aggtcgcccg cattcccgat gaagaggccg gttaccgcct gttgacctgg
     tgggacgggc agggcgcccc ccgactcttc gcctcggcgg cgggcgctct gctcatggag
     cgcgcgtccg gggccgggga ccttgcacag atagcgtggt ccggccagga cgacgaggct
     tgcaggatca attcnggatc cnnttgacat gaggttgccc cgtattcagt gtcgctgatt
     tgtattgtct gaagttgttt ttacgttaag ttgatgcaga tcaattaata cgatacctgc
     gtcataattg attatttgac gtggtttgat ggcctccacg cacgttgtga tatgtagatg
     ataatcatta tcactttacg ggtcctttcc ggtgatccga caggttacgg ggcggcgacc
     tcgcgggttt tcgctattta tgaaaatttt ccggtttaag gcgtttccgt tcttcttcgt
     cataacttaa tgtttttatt taaaataccc tctgaaaaga aaggaaacga caggtgctga
     aagcgaggct ttttggcctc tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa
     tggaagtcaa caagcttgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     ggttggacga accttctgag tttctggtaa cgccgttccg caccccggaa atggtcagcg
     aaccaatcag cagggtcatc gctagccaga tccccgggcg agctcgaatt
//