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ID   LXSH       preliminary; circular DNA; SYN; 6259 BP.
XX
AC   M77239;
XX
DT   03-OCT-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse Moloney leukemia retrovirus vector MMLV LXSH - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6259
RC   LXSH from retroviral LTR & linker & SV40 early promoter & hph gene
RC   PHDCX from retrovirus
RC   from LN
RC   from LNSX
RC   from LNCX
RC   from LXSN
RC   from PHDCX
RC   from LXSHD
RC   from LXSH
RA   Miller A.D., Miller D.G., Garcia J.V., Lynch C.M.;
RT   "The use of retroviral vectors for gene transfer and expression";
RL   Meth. Enzymol. 217:581-599(1993).
XX
CC   NM (LXSH)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (mouse)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Moloney murine leukemia virus)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. LXSN remove 800bp, neo gene/5100bp
FT                   2. E. coli 1142bp, hyg gene
FT                   -> LXSH 6259bp"
FT   LTR             175..763
FT                   /note="RPT Moloney murine leukemia virus (Mo-MuSV)
FT                   5' long terminal repeat"
FT   misc_signal     833..1642
FT                   /note="extended packaging signal"
FT   promoter        1655..2018
FT                   /note="PRO SV40 early gene"
FT   CDS             2042..3079
FT                   /note="ANT E. coli hygromycin phosphotransferase gene,
FT                   hygromycin resistance gene (hyg)"
FT   LTR             3305..3898
FT                   /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT                   3' long terminal repeat"
XX
SQ   Sequence 6259 BP; 1449 A; 1727 C; 1607 G; 1476 T; 0 other;
     gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
     tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
     ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
     agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
     atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
     ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
     acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
     gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
     gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
     atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
     cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
     tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
     tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
     tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
     ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
     ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
     acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccggctg tggaatgtgt
     gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     tttttggagg cctaggcttt tgcaaaaagc tgcccaagct gatccccggg ggcaatgaga
     tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtg atcagcttgg
     cactggttct catgtttgac agcttatcat cgcgaaatga ccgaccaagc gacgcccaac
     ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     gcccacccca acacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
     ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
     ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
     aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     gaggcccttt cgtcttcaa
//