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ID   LXSHD      preliminary; circular DNA; SYN; 6374 BP.
XX
AC   M64753;
XX
DT   09-MAY-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse Moloney leukemia retrovirus vector MMLV LXSHD - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6374
RC   LHDCX from MMLV
RC   LXSHD from MMLV
RA   Stockshlaeder M.A., Storb R., Osborne W.R., Miller A.D.;
RT   "L-histidinol provides effective selection of
RT   retrovirus-vector-transduced keratinocytes without impairing their
RT   proliferative potential";
RL   Hum. Gene Ther. 2:33-39(1991).
XX
CC   NM (LXSHD)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (mouse)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Moloney murine leukemia virus)(Moloney murine sarcoma virus)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. LXSN 5874bp
FT                   2. E. coli 500bp, hisD gene
FT                   -> LXSHD 6374bp"
FT   misc_recomb     174..175
FT                   /note="mouse DNA end/Moloney murine sarcoma virus
FT                   (Mo-MuSV) DNA start"
FT   LTR             175..763
FT                   /note="RPT Moloney murine sarcoma virus (Mo-MuSV)
FT                   5' long terminal repeat"
FT   misc_signal     833..1642
FT                   /note="extended packaging signal"
FT   misc_recomb     1163..1164
FT                   /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT                   end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT   mutation        1223..1225
FT                   /note="GAG atg start codon to TAG stop codon"
FT   misc_recomb     1654..1655
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/SV40 DNA start"
FT   promoter        1655..1994
FT                   /note="PRO SV40 early gene"
FT   misc_recomb     1994..1995
FT                   /note="SV40 DNA end/E. coli hisD DNA start"
FT   CDS             2004..3308
FT                   /note="ANT E. coli histidinol dehydrogenase gene
FT                   histidinol resistance gene (his)"
FT   misc_recomb     3348..3349
FT                   /note="E. coli hisD DNA end/Moloney murine leukemia
FT                   virus (Mo-MuLV) DNA start"
FT   LTR             3420..4013
FT                   /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT                   3' long terminal repeat"
FT   misc_recomb     4082..4083
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/plasmid pBR322 DNA start"
XX
SQ   Sequence 6374 BP; 1482 A; 1780 C; 1628 G; 1484 T; 0 other;
     gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
     tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
     ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
     agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
     atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
     ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
     acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
     gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
     gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
     atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
     cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
     tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
     tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
     tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
     ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
     ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
     acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccggctg tggaatgtgt
     gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     tttttggagg cagatcccgg accatgagct tcaataccct gattgactgg aacagctgta
     gccctgaaca gcagcgtgcg ctgctgacgc gtccggcgat ttccgcctct gacagtatta
     cccggacggt cagcgatatt ctggataatg taaaaacgcg cggtgacgat gccctgcgtg
     aatacagcgc taaatttgat aaaacagaag tgacagcgct acgcgtcacc cctgaagaga
     tcgccgccgc cggcgcgcgt ctgagcgacg aattaaaaca ggcgatgacc gctgccgtca
     aaaatattga aacgttccat tccgcgcaga cgctaccgcc tgtagatgtg gaaacccagc
     caggcgtgcg ttgccagcag gttacgcgtc ccgtctcgtc tgtcggtctg tatattcccg
     gcggctcggc tccgctcttc tcaacggtgc tgatgctggc gacgccggcg cgcattgcgg
     gatgccagaa ggtggttctg tgctcgccgc cgcccatcgc tgatgaaatc ctctatgcgg
     cgcaactgtg tggcgtgcag gaaatcttta acgtcggcgg cgcgcaggcg attgccgctc
     tggccttcgg cagcgagtcc gtaccgaaag tggataaaat ttttggcccc ggcaacgcct
     ttgtaaccga agccaaacgt caggtcagcc agcgtctcga cggcgcggct atcgatatgc
     cagccgggcc gtctgaagta ctggtgatcg cagacagcgg cgcaacaccg gatttcgtcg
     cttctgacct gctctcccag gctgagcacg gcccggattc ccaggtgatc ctgctgacgc
     ctgatgctga cattgcccgc aaggtggcgg aggcggtaga acgtcaactg gcggaactgc
     cgcgcgcgga caccgcccgg caggccctga gcgccagtcg tctgattgtg accaaagatt
     tagcgcagtg cgtcgccatc tctaatcagt atgggccgga acacttaatc atccagacgc
     gcaatgcgcg cgatttggtg gatgcgatta ccagcgcagg ctcggtattt ctcggcgact
     ggtcgccgga atccgccggt gattacgctt ccggaaccaa ccatgtttta ccgacctatg
     gctatactgc tacctgttcc agccttgggt tagcggattt ccagaaacgg atgaccgttc
     aggaactgtc gaaagcgggc ttttccgctc tggcatcaac cattgaaaca ttggcggcgg
     cagaacgtct gaccgcccat aaaaatgccg tgaccctgcg cgtaaacgcc ctcaaggagc
     aagcatgagc actgaaaaca ctctcagcgt cgctgactta gcccgggatc tgcctaggct
     tttgcaaaaa gcttatcgat aaaataaaag attttattta gtctccagaa aaagggggga
     atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc
     tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct
     ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     cctttcgtct tcaa
//