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ID   M13BM21    preliminary; circular DNA; SYN; 7289 BP.
XX
AC   IG0306;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector M13BM21 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7249
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RC   pUC18 from pUC8
RC   pUC19 from pUC9
RA   Yanisch-Perron C., Vieira J., Messing J.;
RT   "Improved M13 phage cloning vectors and host strains: nucleotide
RT   sequences of the M13mp18 and pUC19 vectors";
RL   Gene 33:103-119(1985).
XX
CC   M13 is not lytic; the phage extrude through the cell wall.
CC   The replicative RF I form in the cell is double-stranded and circular,
CC   and acts like a plasmid.
CC   The phage form outside the cell is linear and single-stranded.
CC   NM (M13BM21)
CC   CM (yes)
CC   NA (filamentous ss-DNA)(ds-DNA)
CC   TP (linear)(circular)
CC   ST ()
CC   TY (phage)
CC   SP (Boehringer)
CC   HO (E.coli JM105)(E.coli JM109)
CC   CP ()
CC   FN (cloning oriented)(sequencing dideoxy)(in vitro mutagenesis)
CC   FN (labeled strand-specific DNA)
CC   SE (color blue/white)
CC   PA (M13mp19)
CC   BR (M13BM20)
CC   OF ()
CC   OR (E.coli JM103)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13mp19 remove EcoRI-HindIII 51bp
FT                   \ 6231..6282, MCS/7198bp
FT                   2. oligo EcoRI-HindIII 88bp, MCS
FT                   -> M13BM21 7289bp"
FT   CDS             join(6850..7181,1..831)
FT                   /note="GEN bacteriophage M13 gene II (rf replication
FT                   and nicking)"
FT   CDS             496..831
FT                   /note="GEN bacteriophage M13 gene X"
FT   CDS             843..1106
FT                   /note="GEN bacteriophage M13 gene V (single stranded
FT                   binding protein)"
FT   CDS             1108..1209
FT                   /note="GEN bacteriophage M13 gene VII (morphogenesis)"
FT   CDS             1206..1304
FT                   /note="GEN bacteriophage M13 gene IX (minor coat
FT                   protein)"
FT   misc_binding    1270..1270
FT                   /note="SIT unique SnaBI"
FT   CDS             1301..1522
FT                   /note="GEN bacteriophage M13 gene VIII (major coat
FT                   protein)"
FT   CDS             1579..2853
FT                   /note="GEN bacteriophage M13 gene III (adsorption
FT                   protein)"
FT   misc_binding    1752..1752
FT                   /note="SIT unique BsmI"
FT   CDS             2856..3194
FT                   /note="GEN bacteriophage M13 gene VI (morphogenesis)"
FT   CDS             3196..4242
FT                   /note="GEN bacteriophage M13 gene I (morphogenesis)"
FT   CDS             4220..5500
FT                   /note="GEN bacteriophage M13 gene IV (morphogenesis)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13"
FT   misc_binding    5615..5615
FT                   /note="SIT unique NaeI"
FT   misc_binding    5721..5721
FT                   /note="SIT unique DraIII"
FT   rep_origin      >5615..<5914
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT                   from pBR322 bases 2354..2373"
FT   misc_binding    5914..5914
FT                   /note="SIT unique AvaII"
FT   misc_binding    6002..6002
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli lac operator region"
FT   CDS             <6228..<6935
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 forward -40"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 universal"
FT   misc_binding    6228..6293
FT                   /note="MCS HindIII-NcoI-EcoRV-SphI-PstI-SacI-XbaI-
FT                   SalI-ApaI-KpnI-KspI-EclXI-NotI-BfrI-MluI-BamHI-SmaI-
FT                   EcoRI"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_binding    6408..6408
FT                   /note="SIT unique PvuI"
FT   misc_binding    6437..6437
FT                   /note="SIT unique BglI"
FT   misc_binding    6501..6501
FT                   /note="SIT unique SauI"
FT   misc_binding    0..0
FT                   /note="SIT unique MstII"
FT   misc_binding    6935..6935
FT                   /note="SIT unique BglII"
XX
SQ   Sequence 7289 BP; 1775 A; 1552 C; 1544 G; 2418 T; 0 other;
     aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
     atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
     cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
     gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
     tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
     ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
     tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
     cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
     tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
     aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
     ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
     aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
     atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
     tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
     caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
     ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
     aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
     tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
     ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
     tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
     caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa
     gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
     ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
     gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
     tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
     tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
     ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
     ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
     ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
     cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
     cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
     tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
     acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
     aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
     cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
     tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
     gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
     actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
     tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
     aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
     tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
     gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
     cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
     agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
     attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
     tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
     tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
     tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
     tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
     taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
     tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
     ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
     gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
     tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
     aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
     tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
     cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
     agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
     tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
     tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
     tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
     tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
     cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
     aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
     atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
     acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct
     ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttc
     catgggatat cgcatgcctg cagagctcta gagtcgacgg gcccggtacc gcggccgcac
     ttaagttacg cgtggatccc cgggaattca ctggccgtcg ttttacaacg tcgtgactgg
     gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
     gatcttcctg aggccgatac ggtcgtcgtc ccctcaaact ggcagatgca cggttacgat
     gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg
     gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
     ggccagacgc gaattatttt tgatggcgtt cctattggtt aaaaaatgag ctgatttaac
     aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaata tttgcttata
     caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtacatatg attgacatgc
     tagttttacg attaccgttc atcgattctc ttgtttgctc cagactctca ggcaatgacc
     tgatagcctt tgtagatctc tcaaaaatag ctaccctctc cggcattaat ttatcagcta
     gaacggttga atatcatatt gatggtgatt tgactgtctc cggcctttct cacccttttg
     aatctttacc tacacattac tcaggcattg catttaaaat atatgagggt tctaaaaatt
     tttatccttg cgttgaaata aaggcttctc ccgcaaaagt attacagggt cataatgttt
     ttggtacaac cgatttagct ttatgctctg aggctttatt gcttaatttt gctaattctt
     tgccttgcct gtatgattta ttggatgtt
//