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ID   M13MC18    preliminary; circular DNA; SYN; 2514 BP.
XX
AC   IG5086;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector M13mc18 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   lambda SK4 from EMBL3 & oligo
RC   lambda SK6 from lambda 2001 & oligo
RC   lambda SK10 from lambda SK9 & oligo
RC   lambda SK20 from lambda SK10 & oligo
RC   lambda SK21 from lambda SK9 & lambda GEM11
RC   lambda SK22 from lambda SK9 & oligo
RC   lambda SR9-B from lambda SK18 & oligo
RC   lambda SK23 from lambda SK10 & EMBL3 & lambda SR9-B
RC   lambda SK24 from lambda SK22 & EMBL3 & lambda SR9-B
RC   lambda SK25 from lambda SK22 & oligo
RC   lambda SK27 from lambda SK22
RC   lambda SK40 from lambda SK10 & oligo
RC   lambda SK41 from lambda SK10 & oligo
RC   lambda SK16 from lambda SK15 & oligo
RC   lambda SK17 from lambda SK15 & oligo
RC   lambda SK28 from lambda SK17
RA   Zabarovsky E.R., Klein G., Winberg G.;
RT   "Lambda SK diphasmids: phage lambda vectors for genomic, jumping,
RT   linking and cDNA libraries";
RL   Gene 127:1-14(1993).
XX
RN   [2]
RC   placZ from pBR322 & Charon 4A, lacZ gene
RC   phage EL3/4 from EMBL3 & EMBL4 & placZ
RC   lambda SK3 from EMBL3 & phage EL3/4
RC   phage SK-P from EMBL4 & linker
RC   lambda SK5 from lambda SK3 & phage SK-P
RC   lambda SK1, lambda SK2, lambda SK6 from pHC79 & M13mp18
RC   lambda SK18, lambda SK18' from pHC79 & M13mp18
RC   lambda SK2A from lambda SK18
RC   lambda SK2deltaSR from lambda SK2
RC   lambda SK9 from EMBL3 & lambda SK2deltaSR
RC   lambda SK10 from lambda SK9 & oligo
RC   lambda SK11 from lambda gt11 & oligo
RC   lambda SK12 from lambda SK2A & phage EL3/4
RC   lambda SK15 from lambda SK9 & pUC4K
RC   lambda SK16 from lambda SK15 & oligo
RC   lambda SK17 from lambda SK15 & oligo
RC   lambda SK20 from lambda SK10 & oligo
RC   lambda SK21 from lambda SK9 & pGEM-11Zf+
RC   lambda SK22 from lambda SK9 & oligo
RC   pSR9 from lambda SK2
RC   lambda SK23 from lambda SK10 & oligo & pSR9
RC   lambda SK24 from lambda SK22 & lambda SK23
RC   M13mc18 from M13mp18 & pHC79
RC   M13mc19 from M13mp19 & pHC79
RA   Zabarovsky E.R., Winberg G.;
RT   "Improved methods and new vectors for creating the clone libraries
RT   of genes";
RL   Sov. Sci. Rev. D. Physiochem. Biol. 11:1-65(1992).
XX
RN   [3]
RC   lambda pMYF15 from piAN7 & lambda L47.1AB
RC   lambda pMYF17 from lambda pMYF15 & lambda gtWES.lambdaC
RC   lambda pMYF131 from lambda pMYF17 & pUC19
RC   lambda pSL5 from lambda pMYF131 & lambda L47.1
RC   lambda pMK2 from lambda pMYF131 & ptl gene
RA   Yankovsky N.K., Fonstein M.Y., Laskina S.Y., Bukanov N.O.,
RA   Yakubovich N.V., Ermakova L.M., Rebentish B.A., Janulaitis A.A.,
RA   Debabov V.G.;
RT   "Phasmids as effective and simple tools for construction and
RT   analysis of gene libraries";
RL   Gene 81:203-210(1989).
XX
RN   [4]
RC   SK18 from lambda
RA   Zabarovsky E.R., Winberg G., Boldog F., Turina O.T., Nurbekov M.K.,
RA   Marcsek Z., Erlandsson R., Grigorieva A.Y., Stanbridge E.J.,
RA   Sumegi J., Kisselev L.L., Klein G.;
RT   "New generation of vectors for cloning and mapping of the human
RT   genome";
RL   Adv. Mol. Gen. 4:281-293(1991).
XX
RN   [5]
RC   [lambda 47.1c+ from lambda]
RC   [lambda L47.1, lambda L47.1AB from lambda]
RC   lambda pMYF11 from pBR322 & lambda 47.1c+
RA   Yankovsky N.K., Fonstein M.Y., Debabov V.G.;
RT   "Monomer circular phasmid DNA molecules can be effectively packaged
RT   in vitro and used as vector DNA molecules";
RL   Biotech 85 Europe 85:709-714(1985).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   MCS. oligonucleotide linker.
CC   NM (M13mc18)
CC   CM (yes)
CC   NA (filamentous ss-DNA)(ds-DNA)
CC   TP (linear)(circular)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning oriented)(sequencing dideoxy)(in vitro mutagenesis)
CC   FN (labeled strand-specific DNA)
CC   SE ()
CC   PA (M13mp18)(pHC79)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHC79 EcoRI-SalI 653bp 6523..6524..652,
FT                   \ lambda cos
FT                   Klenow:Klenow
FT                   2. M13mp18 BalI-BglII 1853bp 5082..6935,
FT                   \ M13 ori/lacI/lacZ/MCS
FT                   Klenow:Klenow
FT                   -> M13mc18 2506bp"
FT   -               1..657
FT                   /note="pHC79 6523..6524..655 653bp
FT                   SalI = G^TCGA C
FT                   BalI =    TGG^CCA"
FT   -               658..2514
FT                   /note="M13mp18 5082..6938 1857bp
FT                   BglII = A^GATC T
FT                   EcoRI =      G^AATTC"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2514 BP; 542 A; 683 C; 618 G; 671 T; 0 other;
     aattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta
     aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
     ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
     ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta
     tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc
     cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg
     accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgacca
     gaatgtccct tttattactg gtcgtgtgac tggtgaatct gccaatgtaa ataatccatt
     tcagacgatt gagcgtcaaa atgtaggtat ttccatgagc gtttttcctg ttgcaatggc
     tggcggtaat attgttctgg atattaccag caaggccgat agtttgagtt cttctactca
     ggcaagtgat gttattacta atcaaagaag tattgctaca acggttaatt tgcgtgatgg
     acagactctt ttactcggtg gcctcactga ttataaaaac acttctcaag attctggcgt
     accgttcctg tctaaaatcc ctttaatcgg cctcctgttt agctcccgct ctgattccaa
     cgaggaaagc acgttatacg tgctcgtcaa agcaaccata gtacgcgccc tgtagcggcg
     cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     cggaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cgctggtgaa
     aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc
     ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatacggtc
     gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
     tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     ggcgttccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt
     tctgattatc aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg
     attctcttgt ttgctccaga ctctcaggca atgacctgat agcctttgta gatc
//