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ID   M13MP18    preliminary; circular DNA; SYN; 7249 BP.
XX
AC   X02513; M77815; M11454; VB0018;
XX
DT   02-JUL-1986 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector M13mp18 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7249
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RC   pUC18 from pUC8
RC   pUC19 from pUC9
RA   Yanisch-Perron C., Vieira J., Messing J.;
RT   "Improved M13 phage cloning vectors and host strains: nucleotide
RT   sequences of the M13mp18 and pUC19 vectors";
RL   Gene 33:103-119(1985).
XX
RN   [2]
RC   M13mp1 from M13 & pMG1106
RC   M13mp2 from M13mp1
RC   M13mp5 from M13mp2
RC   M13mp6 from M13mp2
RC   [M13am1, M13am2 from M13]
RC   M13mp61 from M13mp6 & M13am1 & M13am2
RC   M13mp62 from M13mp61
RC   M13mp63 from M13mp62
RC   mWU43 from M13mp2
RC   mWJ22 from M13mp2
RC   M13mp71 from M13mWJ43
RC   M13mp7 from M13mp71 & M13mp63
RC   M13mp8 from M13mp7 & pUC8
RC   M13mp9 from M13mp7 & pUC9
RC   [M13mp8c from M13mp8]
RC   M13mp10 from M13mp8c & pUC12
RC   M13mp11 from M13mp8c & pUC13
RC   M13Ho176 from M13 & lambda h80dhis
RC   M13Gori1 from M13 & phage G4
RC   M13bla from M13 & Tn3
RC   M13blacat from M13 & Tn3 & pACYC184
RC   R199 from phage f1
RC   R208 from R199
RC   R209 from phage f1
RC   R229 from phage f1
RC   fd11 from phage fd & pKB252
RC   fd101 from phage fd & pACYC177
RC   fd103 from phage fd & pACYC177 & pACYC184
RC   fd104 from phage fd & pACYC177
RC   fd106 from phage fd & pACYC177 & pACYC184
RC   fd109 from phage fd & pBR322
RC   fd107 from phage fd & pBR322
RC   fd-tet from phage fd & Tn10
RC   fKN16 from fd-tet
RA   Messing J.;
RT   "New M13 Vectors for Cloning";
RL   Meth. Enzymol. 101:20-78(1983).
XX
RN   [3]
RC   from M13 series
RA   Sanger F., Nicklen S., Coulson A.R.;
RT   "DNA sequencing with chain-terminating inhibitors";
RL   Proc. Natl. Acad. Sci. U.S.A. 74:5463-5468(1977).
XX
RN   [4]
RC   from M13mp5
RC   from M13mp7
RC   from M13mp8
RC   from M13mp9
RA   Zoller M.J., Smith M.;
RT   "Oligonucleotide-directed mutagenesis of DNA fragments cloned into
RT   M13 vectors";
RL   Meth. Enzymol. 100:468-500(1983).
XX
RN   [5]
RC   pHM235 from pBR325 & primer
RA   Hu N.T., Messing J.;
RT   "The making of strand-specific M13 probes";
RL   Gene 17:271-277(1982).
XX
RN   [6]
RC   [M13mp2963 from M13mp2]
RC   pHM232 from pBR325 & M13mp2963
RA   Heidecker G., Messing J., Gronenborn B.;
RT   "A versatile primer for DNA sequencing in the M13mp2 cloning
RT   system";
RL   Gene 10:69-73(1980).
XX
RN   [7]
RC   from M13mp18, M13 gene III
RC   from M13mp19, M13 gene III
RA   Ebright R., Dong Q., Messing J.;
RT   "Corrected nucleotide sequence of M13mp18 gene III";
RL   Gene 114:81-83(1992).
XX
RN   [8]
RC   M13mp18 from M13mp10
RC   M13mp19 from M13mp11
RA   Norrander J., Kempe T., Messing J.;
RT   "Construction of improved M13 vectors using
RT   oligodeoxynucleotide-directed mutagenesis";
RL   Gene 26:101-106(1983).
XX
RN   [9]
RC   from M13mp18
RC   from M13mp19
RA   Hong G.F.;
RT   "A method for sequencing single-stranded cloned DNA in both
RT   directions";
RL   Biosci. Rep. 1:243-252(1981).
XX
RN   [10]
RC   from pBR322, primers
RC   from M13mp2, primers
RC   from M13mp7, primers
RC   from M13bla6, primers
RA   Wallace R.B., Johnson M.J., Suggs S.V., Miyoshi K., Bhatt R.,
RA   Itakura K.;
RT   "A set of synthetic oligodeoxyribonucleotide primers for DNA
RT   sequencing in the plasmid vector pBR322";
RL   Gene 16:21-26(1981).
XX
RN   [11]
RC   M13mp5 from M13mp2
RC   M13mp6 from M13mp2
RA   Messing J.;
RT   "Multipurpose cloning system based on the single-stranded DNA
RT   bacteriophage M13";
RL   Recombinant DNA Technical Bulletin NIH 2:43-48(1979).
XX
RN   [12]
RP   1-7250
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
CC   Size is 7250 for Boehringer.
CC   M13 is not lytic; the phage extrude through the cell wall.
CC   The replicative RF I form in the cell is double-stranded and circular,
CC   and acts like a plasmid.
CC   The phage form outside the cell is linear and single-stranded.
CC   NCBI gi: 58253
CC   NM (M13mp18)
CC   CM (yes)
CC   NA (filamentous ss-DNA)(ds-DNA)
CC   TP (linear)(circular)
CC   ST ()
CC   TY (phage)
CC   SP (Boehringer)(Bio-Rad)(BRL)(NEB)(Pharmacia)(Sigma)(USB)
CC   HO (E.coli JM101)(E.coli JM103)(E.coli JM105)(E.coli CJ236)
CC   HO (E.coli JM107)(E.coli JM109)(E.coli DH5alphaF')(E.coli NM522)
CC   CP ()
CC   FN (cloning oriented)(sequencing dideoxy)(in vitro mutagenesis)
CC   FN (labeled strand-specific DNA)
CC   SE (color blue/white)
CC   PA (M13mp8)
CC   BR (M13mp19)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13mp10 7244bp, MCS
FT                   2. oligo SphI-KpnI 12bp gcatgcggtacc
FT                   -> M13mp18 7249bp"
FT   CDS             join(6850..7181,1..831)
FT                   /note="GEN bacteriophage M13 gene II (rf replication
FT                   and nicking)"
FT   CDS             496..831
FT                   /note="GEN bacteriophage M13 gene X"
FT   CDS             843..1106
FT                   /note="GEN bacteriophage M13 gene V (single stranded
FT                   binding protein)"
FT   CDS             1108..1209
FT                   /note="GEN bacteriophage M13 gene VII (morphogenesis)"
FT   CDS             1206..1304
FT                   /note="GEN bacteriophage M13 gene IX (minor coat
FT                   protein)"
FT   misc_binding    1270..1270
FT                   /note="SIT unique SnaBI"
FT   CDS             1301..1522
FT                   /note="GEN bacteriophage M13 gene VIII (major coat
FT                   protein)"
FT   CDS             1579..2853
FT                   /note="GEN bacteriophage M13 gene III (adsorption
FT                   protein)"
FT   misc_binding    1752..1752
FT                   /note="SIT unique BsmI"
FT   CDS             2856..3194
FT                   /note="GEN bacteriophage M13 gene VI (morphogenesis)"
FT   CDS             3196..4242
FT                   /note="GEN bacteriophage M13 gene I (morphogenesis)"
FT   CDS             4220..5500
FT                   /note="GEN bacteriophage M13 gene IV (morphogenesis)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13 intergenic region
FT                   (M13IG)"
FT   misc_binding    5615..5615
FT                   /note="SIT unique NaeI"
FT   misc_binding    5721..5721
FT                   /note="SIT unique DraIII"
FT   rep_origin      >5615..<5914
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT                   from pBR322 bases 2354..2373"
FT   misc_binding    5914..5914
FT                   /note="SIT unique AvaII"
FT   misc_binding    6002..6002
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli lac operator region"
FT   CDS             <6228..<6935
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 forward -40"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 universal"
FT   misc_binding    6228..6293
FT                   /note="MCS unique EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-
FT                   SalI-PstI-SphI-HindIII"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_binding    6408..6408
FT                   /note="SIT unique PvuI"
FT   misc_binding    6437..6437
FT                   /note="SIT unique BglI"
FT   misc_binding    6501..6501
FT                   /note="SIT unique SauI"
FT   misc_binding    0..0
FT                   /note="SIT unique MstII"
FT   misc_binding    6935..6935
FT                   /note="SIT unique BglII"
XX
SQ   Sequence 7249 BP; 1768 A; 1538 C; 1533 G; 2410 T; 0 other;
     aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
     atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
     cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
     gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
     tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
     ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
     tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
     cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
     tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
     aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
     ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
     aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
     atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
     tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
     caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttc
     tcgtcagggc aagccttatt cactgaatga gcagctttgt tacgttgatt tgggtaatga
     atatccggtt cttgtcaaga ttactcttga tgaaggtcag ccagcctatg cgcctggtct
     gtacaccgtt catctgtcct ctttcaaagt tggtcagttc ggttccctta tgattgaccg
     tctgcgcctc gttccggcta agtaacatgg agcaggtcgc ggatttcgac acaatttatc
     aggcgatgat acaaatctcc gttgtacttt gtttcgcgct tggtataatc gctgggggtc
     aaagatgagt gttttagtgt attctttcgc ctctttcgtt ttaggttggt gccttcgtag
     tggcattacg tattttaccc gtttaatgga aacttcctca tgaaaaagtc tttagtcctc
     aaagcctctg tagccgttgc taccctcgtt ccgatgctgt ctttcgctgc tgagggtgac
     gatcccgcaa aagcggcctt taactccctg caagcctcag cgaccgaata tatcggttat
     gcgtgggcga tggttgttgt cattgtcggc gcaactatcg gtatcaagct gtttaagaaa
     ttcacctcga aagcaagctg ataaaccgat acaattaaag gctccttttg gagccttttt
     ttttggagat tttcaacgtg aaaaaattat tattcgcaat tcctttagtt gttcctttct
     attctcactc cgctgaaact gttgaaagtt gtttagcaaa accccataca gaaaattcat
     ttactaacgt ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggttgtc
     tgtggaatgc tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat
     gggttcctat tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt
     ctgagggtgg cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta
     ttccgggcta tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa
     accccgctaa tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc
     agaataatag gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc
     aaggcactga ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt
     atgacgctta ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgaag
     atccattcgt ttgtgaatat caaggccaat cgtctgacct gcctcaacct cctgtcaatg
     ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc tctgagggtg
     gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg
     attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg
     aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg
     ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg
     gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt
     taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt
     ttgtctttag cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat
     tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt
     ttgctaacat actgcgtaat aaggagtctt aatcatgcca gttcttttgg gtattccgtt
     attattgcgt ttcctcggtt tccttctggt aactttgttc ggctatctgc ttacttttct
     taaaaagggc ttcggtaaga tagctattgc tatttcattg tttcttgctc ttattattgg
     gcttaactca attcttgtgg gttatctctc tgatattagc gctcaattac cctctgactt
     tgttcagggt gttcagttaa ttctcccgtc taatgcgctt ccctgttttt atgttattct
     ctctgtaaag gctgctattt tcatttttga cgttaaacaa aaaatcgttt cttatttgga
     ttgggataaa taatatggct gtttattttg taactggcaa attaggctct ggaaagacgc
     tcgttagcgt tggtaagatt caggataaaa ttgtagctgg gtgcaaaata gcaactaatc
     ttgatttaag gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg cctcgcgttc
     ttagaatacc ggataagcct tctatatctg atttgcttgc tattgggcgc ggtaatgatt
     cctacgatga aaataaaaac ggcttgcttg ttctcgatga gtgcggtact tggtttaata
     cccgttcttg gaatgataag gaaagacagc cgattattga ttggtttcta catgctcgta
     aattaggatg ggatattatt tttcttgttc aggacttatc tattgttgat aaacaggcgc
     gttctgcatt agctgaacat gttgtttatt gtcgtcgtct ggacagaatt actttacctt
     ttgtcggtac tttatattct cttattactg gctcgaaaat gcctctgcct aaattacatg
     ttggcgttgt taaatatggc gattctcaat taagccctac tgttgagcgt tggctttata
     ctggtaagaa tttgtataac gcatatgata ctaaacaggc tttttctagt aattatgatt
     ccggtgttta ttcttattta acgccttatt tatcacacgg tcggtatttc aaaccattaa
     atttaggtca gaagatgaaa ttaactaaaa tatatttgaa aaagttttct cgcgttcttt
     gtcttgcgat tggatttgca tcagcattta catatagtta tataacccaa cctaagccgg
     aggttaaaaa ggtagtctct cagacctatg attttgataa attcactatt gactcttctc
     agcgtcttaa tctaagctat cgctatgttt tcaaggattc taagggaaaa ttaattaata
     gcgacgattt acagaagcaa ggttattcac tcacatatat tgatttatgt actgtttcca
     ttaaaaaagg taattcaaat gaaattgtta aatgtaatta attttgtttt cttgatgttt
     gtttcatcat cttcttttgc tcaggtaatt gaaatgaata attcgcctct gcgcgatttt
     gtaacttggt attcaaagca atcaggcgaa tccgttattg tttctcccga tgtaaaaggt
     actgttactg tatattcatc tgacgttaaa cctgaaaatc tacgcaattt ctttatttct
     gttttacgtg ctaataattt tgatatggtt ggttcaattc cttccataat tcagaagtat
     aatccaaaca atcaggatta tattgatgaa ttgccatcat ctgataatca ggaatatgat
     gataattccg ctccttctgg tggtttcttt gttccgcaaa atgataatgt tactcaaact
     tttaaaatta ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt gtttgtaaag
     tctaatactt ctaaatcctc aaatgtatta tctattgacg gctctaatct attagttgtt
     agtgcaccta aagatatttt agataacctt cctcaattcc tttctactgt tgatttgcca
     actgaccaga tattgattga gggtttgata tttgaggttc agcaaggtga tgctttagat
     ttttcatttg ctgctggctc tcagcgtggc actgttgcag gcggtgttaa tactgaccgc
     ctcacctctg ttttatcttc tgctggtggt tcgttcggta tttttaatgg cgatgtttta
     gggctatcag ttcgcgcatt aaagactaat agccattcaa aaatattgtc tgtgccacgt
     attcttacgc tttcaggtca gaagggttct atctctgttg gccagaatgt cccttttatt
     actggtcgtg tgactggtga atctgccaat gtaaataatc catttcagac gattgagcgt
     caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt
     ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt
     actaatcaaa gaagtattgc tacaacggtt aatttgcgtg atggacagac tcttttactc
     ggtggcctca ctgattataa aaacacttct caagattctg gcgtaccgtt cctgtctaaa
     atccctttaa tcggcctcct gtttagctcc cgctctgatt ccaacgagga aagcacgtta
     tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
     caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     caggcggtga agggcaatca gctgttgccc gtctcgctgg tgaaaagaaa aaccaccctg
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
     cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg
     ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
     cggaaagctg gctggagtgc gatcttcctg aggccgatac ggtcgtcgtc ccctcaaact
     ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca
     atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
     atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt cctattggtt
     aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     aatttaaata tttgcttata caatcttcct gtttttgggg cttttctgat tatcaaccgg
     ggtacatatg attgacatgc tagttttacg attaccgttc atcgattctc ttgtttgctc
     cagactctca ggcaatgacc tgatagcctt tgtagatctc tcaaaaatag ctaccctctc
     cggcattaat ttatcagcta gaacggttga atatcatatt gatggtgatt tgactgtctc
     cggcctttct cacccttttg aatctttacc tacacattac tcaggcattg catttaaaat
     atatgagggt tctaaaaatt tttatccttg cgttgaaata aaggcttctc ccgcaaaagt
     attacagggt cataatgttt ttggtacaac cgatttagct ttatgctctg aggctttatt
     gcttaatttt gctaattctt tgccttgcct gtatgattta ttggatgtt
//