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ID   M13MP19    preliminary; circular DNA; SYN; 7250 BP.
XX
AC   L08821; VB0019; IG0006;
XX
DT   20-NOV-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector M13mp19 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7249
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RC   pUC18 from pUC8
RC   pUC19 from pUC9
RA   Yanisch-Perron C., Vieira J., Messing J.;
RT   "Improved M13 phage cloning vectors and host strains: nucleotide
RT   sequences of the M13mp18 and pUC19 vectors";
RL   Gene 33:103-119(1985).
XX
RN   [2]
RP   1-7250
RC   M13mp18 from M13mp8
RC   M13mp19 from M13mp9
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [3]
RC   M13mp18 from M13mp10
RC   M13mp19 from M13mp11
RA   Norrander J., Kempe T., Messing J.;
RT   "Construction of improved M13 vectors using
RT   oligodeoxynucleotide-directed mutagenesis";
RL   Gene 26:101-106(1983).
XX
CC   M13 is not lytic; the phage extrude through the cell wall.
CC   The replicative RF I form in the cell is double-stranded and
CC   circular, and acts like a plasmid.
CC   The phage form outside the cell is linear and single-stranded.
CC   NM (M13mp19)
CC   CM (yes)
CC   NA (filamentous ss-DNA)(ds-DNA)
CC   TP (linear)(circular)
CC   ST ()
CC   TY (phage)
CC   SP (Boehringer)(Bio-Rad)(BRL)(IBI)(NEB)(Pharmacia)(Sigma)
CC   HO (E.coli JM101)(E.coli JM103)(E.coli JM105)(E.coli CJ236)
CC   HO (E.coli JM107)(E.coli JM109)(E.coli DH5alphaF')(E.coli NM522)
CC   CP ()
CC   FN (cloning oriented)(sequencing dideoxy)(in vitro mutagenesis)
CC   FN (labeled strand-specific DNA)
CC   SE (color blue/white)
CC   PA (M13mp9)(M13mp11)
CC   BR (M13mp18)
CC   OF (pUC19)(pUC19c)(PhageScript SK)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13mp11 7244bp, MCS
FT                   2. oligo SphI-KpnI 12bp gcatgcggtacc
FT                   -> M13mp19 7250bp"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene II (rf replication
FT                   and nicking)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene X"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene V (single stranded
FT                   binding protein)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene VII (morphogenesis)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene IX (minor coat
FT                   protein)"
FT   misc_binding    0..0
FT                   /note="SIT unique SnaBI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene VIII (major coat
FT                   protein)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene III (adsorption
FT                   protein)"
FT   misc_binding    0..0
FT                   /note="SIT unique BsmI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene VI (morphogenesis)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene I (morphogenesis)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage M13 gene IV (morphogenesis)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13 intergenic region
FT                   (M13IG)"
FT   misc_binding    0..0
FT                   /note="SIT unique NaeI"
FT   misc_binding    0..0
FT                   /note="SIT unique DraIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT                   from pBR322 bases 2354..2373"
FT   misc_binding    0..0
FT                   /note="SIT unique AvaII"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli lac operator region"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 forward -40"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 universal"
FT   misc_binding    6234..6290
FT                   /note="MCS unique HindIII-SphI-PstI-SalI-XbaI-BamHI-
FT                   SmaI-KpnI-SacI-EcoRI"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_binding    0..0
FT                   /note="SIT unique BglI"
FT   misc_binding    0..0
FT                   /note="SIT unique SauI"
FT   misc_binding    0..0
FT                   /note="SIT unique MstII"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   misc_feature    1..5868
FT                   /note="from bacteriophage M13"
FT   misc_feature    5869..6231
FT                   /note="from E. coli lac operon"
FT   misc_feature    6234..6290
FT                   /note="MCS M13MP19/pUC19 polylinker"
FT   misc_feature    6291..6711
FT                   /note="from E. coli lac operon"
FT   misc_feature    6712..7250
FT                   /note="from bacteriophage M13"
XX
SQ   Sequence 7250 BP; 1768 A; 1540 C; 1531 G; 2411 T; 0 other;
     aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
     atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
     cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
     gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
     tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
     ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
     tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
     cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
     tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
     aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
     ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
     aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
     atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
     tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
     caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
     ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
     aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
     tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
     ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
     tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
     caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa
     gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
     ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
     gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
     tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
     tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
     ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
     ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
     ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
     cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
     cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
     tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
     acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
     aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
     cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
     tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
     gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
     actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
     tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
     aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
     tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
     gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
     cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
     agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
     attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
     tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
     tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
     tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
     tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
     taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
     tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
     ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
     gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
     tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
     aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
     tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
     cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
     agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
     tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
     tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
     tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
     tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
     cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
     aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
     atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
     acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct
     ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
     catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc
     gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
     ccggaaagct ggctggagtg cgatcttcct gaggccgata cggtcgtcgt cccctcaaac
     tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc
     aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
     gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt tcctattggt
     taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga ttatcaaccg
     gggtacatat gattgacatg ctagttttac gattaccgtt catcgattct cttgtttgct
     ccagactctc aggcaatgac ctgatagcct ttgtagatct ctcaaaaata gctaccctct
     ccggcattaa tttatcagct agaacggttg aatatcatat tgatggtgat ttgactgtct
     ccggcctttc tcaccctttt gaatctttac ctacacatta ctcaggcatt gcatttaaaa
     tatatgaggg ttctaaaaat ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag
     tattacaggg tcataatgtt tttggtacaa ccgatttagc tttatgctct gaggctttat
     tgcttaattt tgctaattct ttgccttgcc tgtatgattt attggatgtt
//