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ID   MMLV       standard; RNA; VRL; 8332 BP.
XX
AC   J02255; J02256; J02257;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse Moloney murine leukemia virus MMLV - complete.
XX
KW   cloning vector.
XX
OS   Moloney murine leukemia virus (MO-MuLV)
OC   Viridae; ss-RNA enveloped viruses; Positive strand RNA viruses;
OC   Retroviridae; Oncovirinae; Type C oncovirus group;
OC   Mammalian type C oncoviruses; Murine leukemia viruses.
XX
RN   [1]
RP   1-8332
RC   MMLV from mouse
RA   Shinnick T.M., Lerner R.A., Sutcliffe J.G.;
RT   "Nucleotide sequence of Moloney murine leukaemia virus";
RL   Nature 293:543-548(1981).
XX
RN   [2]
RP   5777-5890
RC   MMLV from mouse
RA   Van Beveren C., van Straaten F., Galleshaw J.A., Verma I.M.;
RT   "Nucleotide sequence of the genome of a murine sarcoma virus";
RL   Cell 27:97-108(1981).
XX
RN   [3]
RP   380-629
RC   MMLV from mouse
RA   Schwartzberg P., Colicelli J., Goff P.;
RT   "Deletion mutants of Moloney murine leukemia virus which lack
RT   glycosylated gag protein are replication competent";
RL   J. Virol. 46:538-546(1983).
XX
RN   [4]
RC   gag region deletion mutant analysis
RC   MMLV from mouse
RA   Crawford S., Goff S.P.;
RT   "Mutations in gag proteins P12 and P15 of Moloney murine leukemia
RT   virus block early stages of infection";
RL   J. Virol. 49:909-917(1984).
XX
RN   [5]
RC   gag-pol region deletion mutant analysis
RC   MMLV from mouse
RA   Schwartzberg P., Colicelli J., Gordon M.L., Goff S.;
RT   "Mutations in the gag gene of Moloney murine leukemia virus:
RT   Effects on production of virions and reverse transcriptase";
RL   J. Virol. 49:918-924(1984).
XX
RN   [6]
RP   1560-2559
RC   MMLV from mouse
RA   Miller A.D., Verma I.M.;
RT   "Two base changes restore infectivity to a noninfectious molecular
RT   clone of moloney murine leukemia virus (pMLV-1)";
RL   J. Virol. 49:214-222(1984).
XX
RN   [7]
RP   563-744
RC   MMLV from mouse
RA   Bender M.A., Palmer T.D., Gelinas R.E., Miller A.D.;
RT   "Evidence that the packaging signal of Moloney murine leukemia
RT   virus extends into the gag region";
RL   J. Virol. 61:1639-1646(1987).
XX
CC   Main features of genome: r(1-68) - u5(69-145) - gag - pol - env -
CC   u3(7816-8264) - r(8265-8332). 594 bp LTR of provirus is u3-r-u5.
CC   Three 10 bp direct repeats at 7923, 7998 and 8073 may be associated
CC   with a 75 bp duplication at 7933-8007 and 8008-8082, relative to a
CC   known provirus sequence. The mRNAs have not yet been mapped. The
CC   gag gene product is involved in the assembly and release of virion
CC   particles. The gag protein is cleaved into four products with
CC   distinct biochemical properties after release from the cell surface
CC   [5].
XX
CC   NM (MMLV)
CC   CM (yes)
CC   NA (RNA)
CC   TP (linear)
CC   ST ()
CC   TY (virus)
CC   SP ()
CC   HO (mouse)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (mouse)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. mouse
FT                   -> MMLV 8332bp [Moloney murine leukemia virus]"
FT   mRNA            1..8332
FT                   /note="Mo-Mulv mRNA"
FT   repeat_region   1..145
FT                   /note="5' LTR"
FT   CDS             621..2237
FT                   /note="gag polyprotein pr65"
FT   CDS             624..1013
FT                   /note="protein p15"
FT   CDS             1014..1265
FT                   /note="protein p12"
FT   CDS             1266..2054
FT                   /note="protein p30 (major structural protein)"
FT   CDS             2055..2222
FT                   /note="protein p10 (histone-like protein)"
FT   CDS             2238..5837
FT                   /note="gag/pol precursor"
FT   CDS             2238..5837
FT                   /note="pol polyprotein"
FT   CDS             5777..7774
FT                   /note="env polyprotein"
FT   CDS             5876..7183
FT                   /note="protein gp70"
FT   CDS             7184..7723
FT                   /note="protein p15e"
FT   CDS             7724..7771
FT                   /note="r protein"
FT   repeat_region   7816..8332
FT                   /note="3' LTR"
XX
SQ   Sequence 8332 BP; 2143 A; 2395 C; 2025 G; 1769 T; 0 other;
     gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga
     ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct
     ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg
     gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg
     atcgttttgg actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag
     acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa
     gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     ttctgtattt gtctgagaat atgggccaga ctgttaccac tcccttaagt ttgaccttag
     gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     tcactccttc tctaggcgcc aaacctaaac ctcaagttct ttctgacagt ggggggccgc
     tcatcgacct acttacagaa gaccccccgc cttataggga cccaagacca cccccttccg
     acagggacgg aaatggtgga gaagcgaccc ctgcgggaga ggcaccggac ccctccccaa
     tggcatctcg cctacgtggg agacgggagc cccctgtggc cgactccact acctcgcagg
     cattccccct ccgcgcagga ggaaacggac agcttcaata ctggccgttc tcctcttctg
     acctttacaa ctggaaaaat aataaccctt ctttttctga agatccaggt aaactgacag
     ctctgatcga gtctgttctc atcacccatc agcccacctg ggacgactgt cagcagctgt
     tggggactct gctgaccgga gaagaaaaac aacgggtgct cttagaggct agaaaggcgg
     tgcggggcga tgatgggcgc cccactcaac tgcccaatga agtcgatgcc gcttttcccc
     tcgagcgccc agactgggat tacaccaccc aggcaggtag gaaccaccta gtccactatc
     gccagttgct cctagcgggt ctccaaaacg cgggcagaag ccccaccaat ttggccaagg
     taaaaggaat aacacaaggg cccaatgagt ctccctcggc cttcctagag agacttaagg
     aagcctatcg caggtacact ccttatgacc ctgaggaccc agggcaagaa actaatgtgt
     ctatgtcttt catttggcag tctgccccag acattgggag aaagttagag aggttagaag
     atttaaaaaa caagacgctt ggagatttgg ttagagaggc agaaaagatc tttaataaac
     gagaaacccc ggaagaaaga gaggaacgta tcaggagaga aacagaggaa aaagaagaac
     gccgtaggac agaggatgag cagaaagaga aagaaagaga tcgtaggaga catagagaga
     tgagcaagct attggccact gtcgttagtg gacagaaaca ggatagacag ggaggagaac
     gaaggaggtc ccaactcgat cgcgaccagt gtgcctactg caaagaaaag gggcactggg
     ctaaagattg tcccaagaaa ccacgaggac ctcggggacc aagaccccag acctccctcc
     tgaccctaga tgactaggga ggtcagggtc aggagccccc ccctgaaccc aggataaccc
     tcaaagtcgg ggggcaaccc gtcaccttcc tggtagatac tggggcccaa cactccgtgc
     tgacccaaaa tcctggaccc ctaagtgata agtctgcctg ggtccaaggg gctactggag
     gaaagcggta tcgctggacc acggatcgca aagtacatct agctaccggt aaggtcaccc
     actctttcct ccatgtacca gactgtccct atcctctgtt aggaagagat ttgctgacta
     aactaaaagc ccaaatccac tttgagggat caggagctca ggttatggga ccaatggggc
     agcccctgca agtgttgacc ctaaatatag aagatgagca tcggctacat gagacctcaa
     aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag gcctgggcgg
     aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct ctgaaagcaa
     cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga ctggggatca
     agccccacat acagagactg ttggaccagg gaatactggt accctgccag tccccctgga
     acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct gtccaggatc
     tgagagaagt caacaagcgg gtggaagaca tccaccccac cgtgcccaac ccttacaacc
     tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta aaggatgcct
     ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag tggagagatc
     cagagatggg aatctcagga caattgacct ggaccagact cccacagggt ttcaaaaaca
     gtcccaccct gtttgatgag gcactgcaca gagacctagc agacttccgg atccagcacc
     cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact tctgagctag
     actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg tatcgggcct
     cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat cttctaaaag
     agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag cctactccga
     agacccctcg acaactaagg gagttcctag ggacggcagg cttctgtcgc ctctggatcc
     ctgggtttgc agaaatggca gcccccttgt accctctcac caaaacgggg actctgttta
     attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt ctaactgccc
     cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac gagaagcagg
     gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc
     tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg atggtagcag
     ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca ctagtcattc
     tggcccccca tgcagtagag gcactagtca aacaaccccc cgaccgctgg ctttccaacg
     cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag ttcggaccgg
     tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg caacacaact
     gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac cagccgctcc
     cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag ggacagcgta
     aggcgggagc tgcggtgacc accgagaccg aggtaatctg ggctaaagcc ctgccagccg
     ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag atggcagaag
     gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc catatccatg
     gagaaatata cagaaggcgt gggttgctca catcagaagg caaagagatc aaaaataaag
     acgagatctt ggccctacta aaagccctct ttctgcccaa aagacttagc ataatccatt
     gtccaggaca tcaaaaggga cacagcgccg aggctagagg caaccggatg gctgaccaag
     cggcccgaaa ggcagccatc acagagactc cagacacctc taccctcctc atagaaaatt
     catcacccta cacctcagaa cattttcatt acacagtgac tgatataaag gacctaacca
     agttgggggc catttatgat aaaacaaaga agtattgggt ctaccaagga aaacctgtga
     tgcctgacca gtttactttt gaattattag actttcttca tcagctgact cacctcagct
     tctcaaaaat gaaggctctc ctagagagaa gccacagtcc ctactacatg ctgaaccggg
     atcgaacact caaaaatatc actgagacct gcaaagcttg tgcacaagtc aacgccagca
     agtctgccgt taaacaggga actagggtcc gcgggcatcg gcccggcact cattgggaga
     tcgatttcac cgagataaag cccggattgt atggctataa atatcttcta gtttttatag
     ataccttttc tggctggata gaagccttcc caaccaagaa agaaaccgcc aaggtcgtaa
     ccaagaagct actagaggag atcttcccca ggttcggcat gcctcaggta ttgggaactg
     acaatgggcc tgccttcgtc tccaaggtga gtcagacagt ggccgatctg ttggggattg
     attggaaatt acattgtgca tacagacccc aaagctcagg ccaggtagaa agaatgaata
     gaaccatcaa ggagacttta actaaattaa cgcttgcaac tggctctaga gactgggtgc
     tcctactccc cttagccctg taccgagccc gcaacacgcc gggcccccat ggcctcaccc
     catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga
     caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc
     acgaagtctg gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac
     ctcaccctta ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag
     aacctcgctg gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag
     acggcatcgc agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac
     catcctctag actgacatgg cgcgttcaac gctctcaaaa ccccttaaaa ataaggttaa
     cccgcgaggc cccctaatcc ccttaattct tctgatgctc agaggggtca gtactgcttc
     gcccggctcc agtcctcatc aagtctataa tatcacctgg gaggtaacca atggagatcg
     ggagacggta tgggcaactt ctggcaacca ccctctgtgg acctggtggc ctgaccttac
     cccagattta tgtatgttag cccaccatgg accatcttat tgggggctag aatatcaatc
     ccctttttct tctcccccgg ggcccccttg ttgctcaggg ggcagcagcc caggctgttc
     cagagactgc gaagaacctt taacctccct cacccctcgg tgcaacactg cctggaacag
     actcaagcta gaccagacaa ctcataaatc aaatgaggga ttttatgttt gccccgggcc
     ccaccgcccc cgagaatcca agtcatgtgg gggtccagac tccttctact gtgcctattg
     gggctgtgag acaaccggta gagcttactg gaagccctcc tcatcatggg atttcatcac
     agtaaacaac aatctcacct ctgaccaggc tgtccaggta tgcaaagata ataagtggtg
     caacccctta gttattcggt ttacagacgc cgggagacgg gttacttcct ggaccacagg
     acattactgg ggcttacgtt tgtatgtctc cggacaagat ccagggctta catttgggat
     ccgactcaga taccaaaatc taggaccccg cgtcccaata gggccaaacc ccgttctggc
     agaccaacag ccactctcca agcccaaacc tgttaagtcg ccttcagtca ccaaaccacc
     cagtgggact cctctctccc ctacccaact tccaccggcg ggaacggaaa ataggctgct
     aaacttagta gacggagcct accaagccct caacctcacc agtcctgaca aaacccaaga
     gtgctggttg tgtctagtag cgggaccccc ctactacgaa ggggttgccg tcctgggtac
     ctactccaac catacctctg ctccagccaa ctgctccgtg gcctcccaac acaagttgac
     cctgtccgaa gtgaccggac agggactctg cataggagca gttcccaaaa cacatcaggc
     cctatgtaat accacccaga caagcagtcg agggtcctat tatctagttg cccctacagg
     taccatgtgg gcttgtagta ccgggcttac tccatgcatc tccaccacca tactgaacct
     taccactgat tattgtgttc ttgtcgaact ctggccaaga gtcacctatc attcccccag
     ctatgtttac ggcctgtttg agagatccaa ccgacacaaa agagaaccgg tgtcgttaac
     cctggcccta ttattgggtg gactaaccat ggggggaatt gccgctggaa taggaacagg
     gactactgct ctaatggcca ctcagcaatt ccagcagctc caagccgcag tacaggatga
     tctcagggag gttgaaaaat caatctctaa cctagaaaag tctctcactt ccctgtctga
     agttgtccta cagaatcgaa ggggcctaga cttgttattt ctaaaagaag gagggctgtg
     tgctgctcta aaagaagaat gttgcttcta tgcggaccac acaggactag tgagagacag
     catggccaaa ttgagagaga ggcttaatca gagacagaaa ctgtttgagt caactcaagg
     atggtttgag ggactgttta acagatcccc ttggtttacc accttgatat ctaccattat
     gggacccctc attgtactcc taatgatttt gctcttcgga ccctgcattc ttaatcgatt
     agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata
     tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct
     ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
     aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
     ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
     tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg ca
//