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ID   MUA3       preliminary; DNA; SYN; 4695 BP.
XX
AC   IG0012; ATCC37069;
XX
DT   20-NOV-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli cosmid vector MUA-3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   MUA-3, MUA-5, MUA-10 from pBR322 & lambda, cI857
RA   Meyerowitz E.M., Guild G.M., Prestidge L.S., Hogness D.S.;
RT   "A new high-capacity cosmid vector and its use";
RL   Gene 11:271-282(1980).
XX
CC   ATCC size is 4300 bp.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (MUA-3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning up to 48000 bp)
CC   SE ()
CC   PA (pBR322)(lambda)
CC   BR (MUA-10)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda EcoRI 48502bp 31748..31748, linearize
FT                   HindII-HindII 403bp 48299..48502..200, lambda cos
FT                   C-tail 3' end
FT                   2. pBR322 PstI 4361bp 3612..3612, amp
FT                   G-tail 5' end
FT                   -> MUA-3 4695bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI-EcoRI-BamHI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   rep_origin      2530..2530
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    3614..4015
FT                   /note="SIT bacteriophage lambda cos region,
FT                   in HindII-HindII fragment"
XX
SQ   Sequence 4695 BP; 1057 A; 1271 C; 1215 G; 1152 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc
     acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
     atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg
     cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac
     gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
     ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
     cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
     aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
     tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
     accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     tcattacccc catgaacaga aattccccct tacacggagg catcaagtga ccaaacagga
     aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     gcagacatga ggttgccccg tattcagtgt cgctgatttg tattgtctga agttgttttt
     acgttaagtt gatgcagatc aattaatacg atacctgcgt cataattgat tatttgacgt
     ggtttgatgg cctccacgca cgttgtgata tgtagatgat aatcattata actttacggg
     tactttccgg tgatccgaca ggttacgggg cggcgacctc gcgggttttc gctatttatg
     aaaattttcc ggtttaagcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa
     aataccctct gaaaagaaag gaaacgacag gtgctgaaag cgaggctttt tggcctctgt
     cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg aagtcggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     tttcgtcttc aagaa
//