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ID   N2         preliminary; circular DNA; SYN; 3239 BP.
XX
AC   IG7002;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse Moloney leukemia retrovirus vector N2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   DE-NA/X from M-MuLV & human ADA gene & Tn5, neo gene
RC   DE-NA/S from M-MuLV & human ADA gene & Tn5, neo gene
RC   N4 from M-MuLV & Tn5, neo gene
RC   N1, N2, N3 from M-MuLV & Tn5, neo gene
RA   Armentano D., Yu S.F., Kantoff P.W., Von Ruden T., Anderson W.F.,
RA   Gilboa E.;
RT   "Effect of internal viral sequences on the utility of retroviral
RT   vectors";
RL   J. Virol. 61:1647-1650(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (N2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (mouse)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Moloney murine leukemia virus)(Tn5)
CC   BR ()
CC   OF (LNL6)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. MoMuLV remove 1039..7673, 1698bp
FT                   2. Tn5 BglII-BamHI 1541bp 1516..3057, neo gene
FT                   -> N2 3239bp [MoMuLV LTR]"
FT   -               1..1038
FT                   /note="MoMuLV 1..1038 1038bp
FT                   BglII = A^GATCT"
FT   -               1039..2579
FT                   /note="Tn5 1516..3056 1541bp
FT                   BamHI = G^GATCC"
FT   -               2580..3239
FT                   /note="MoMuLV 7673..8332 660bp"
FT   misc_recomb     0..0
FT                   /note="mouse DNA end/Moloney murine leukemia virus
FT                   (Mo-MuLV) DNA start"
FT   LTR             0..0
FT                   /note="Moloney murine leukemia virus (Mo-MuLV)
FT                   5' long terminal repeat"
FT   misc_signal     0..0
FT                   /note="extended packaging signal"
FT   misc_feature    0..0
FT                   /note="MoMLV gag gene CTG start codon"
FT   CDS             0..0
FT                   /note="MoMLV gag gene"
FT   misc_recomb     0..0
FT                   /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT                   end/transposon Tn5 DNA start"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)"
FT   misc_recomb     0..0
FT                   /note="transposon Tn5 DNA end/Moloney murine
FT                   leukemia virus (MoMuLV) DNA start"
FT   LTR             0..0
FT                   /note="Moloney murine leukemia virus (Mo-MuLV)
FT                   3' long terminal repeat"
XX
SQ   Sequence 3239 BP; 642 A; 946 C; 917 G; 734 T; 0 other;
     gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga
     ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct
     ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg
     gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg
     atcgttttgg actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag
     acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa
     gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     ttctgtattt gtctgagaat atgggccaga ctgttaccac tcccttaagt ttgaccttag
     gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     tcactccttc tctaggcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     cgggctcgat cccctcgcga gttggttcag ctgctgcctg aggctggacg acctcgcgga
     gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct atccgcgcat
     ccatgccccc gaactgcagg agtggggagg cacgatggcc gctttggtcg acccggacgg
     gacgctcctg cgcctgatac agaacgaatt gcttgcaggc atctcatgag tgtgtcttcc
     cgttttccgc ctgaggtcac tgcgtggatg gagcgctggc gcctgctgcg cgacggcgag
     ctgctcacca cccactcgag ctggatactt cccgtccgcc agggggacat gccggcgatg
     ctgaaggtcg cgcgcattcc cgatgaagag gccggttacc gcctgttgac ctggtgggac
     gggcagggcg ccgcccgagt cttcgcctcg gcggcgggcg ctctgctcat ggagcgcgcg
     tccggggccg gggaccttgc acagatagcg tggtccggcc aggacgacga ggcttgcaga
     atcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc
     aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa aagattttat
     ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat
     caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgca
//