back Return to this vector's summary.
ID   P2BAC      preliminary; circular DNA; SYN; 7125 BP.
XX
AC   IG1020;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector p2Bac - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p2Bac from baculovirus
RC   p2Blue from p2Bac
RC   pBlueBac from AcNPV
RC   pBlueBac2 from AcNPV
RC   pBlueBacIII from AcNPV
RC   pBlueBacHisA from AcNPV
RC   pBlueBacHisB from AcNPV
RC   pBlueBacHisC from AcNPV
RC   pVL1392, pVL1393 from pAc series
RA   ;
RT   ;
RL   The Digest 5:2-2(1992).
XX
CC   NM (p2Bac)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (transfer)(expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF (p2Blue)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. AcMNPV, p10 promoter/polyhedrin gene/polyA
FT                   2. pBR322, ori/amp gene
FT                   3. BGH gene polyA
FT                   -> p2Bac 7125bp"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   polyA_signal    0..0
FT                   /note="PLA AcMNPV polyhedrin gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    226..226
FT                   /note="SIT SphI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    2342..2342
FT                   /note="SIT XbaI"
FT   polyA_signal    0..0
FT                   /note="PLA BGH gene"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   promoter        complement(0..0)
FT                   /note="PRO AcMNPV p10 gene"
XX
SQ   Sequence 7125 BP; 1913 A; 1554 C; 1645 G; 2013 T; 0 other;
     gcagttcgtt gacgccttcc tccgtgtggc cgaacacgtc gagcgggtgg tcgatgacca
     gcggcgtgcc gcacgcgacg cacaagtatc tgtacaccga atgatcgtcg ggcgaaggca
     cgtcggcctc caagtggcaa tattggcaaa ttcgaaaata tatacagttg ggttgtttgc
     gcatatctat cgtggcgttg ggcatgtacg tccgaacgtt gatttgcatg caagccgaaa
     ttaaatcatt gcgattagtg cgattaaaac gttgtacatc ctcgctttta atcatgccgt
     cgattaaatc gcgcaatcga gtcaagtgat caaagtgtgg aataatgttt tctttgtatt
     cccgagtcaa gcgcagcgcg tattttaaca aactagccat cttgtaagtt agtttcattt
     aatgcaactt tatccaataa tatattatgt atcgcacgtc aagaattaac aatgcgcccg
     ttgtcgcatc tcaacacgac tatgatagag atcaaataaa gcgcgaatta aatagcttgc
     gacgcaacgt gcacgatctg tgcacgcgtt ccggcacgag ctttgattgt aataagtttt
     tacgaagcga tgacatgacc cccgtagtga caacgatcac gcccaaaaga actgccgact
     acaaaattac cgagtatgtc ggtgacgtta aaactattaa gccatccaat cgaccgttag
     tcgaatcagg accgctggtg cgagaagccg cgaagtatgg cgaatgcatc gtataacgtg
     tggagtccgc tcattagagc gtcatgttta gacaagaaag ctacatattt aattgatccc
     gatgatttta ttgataaatt gaccctaact ccatacacgg tattctacaa tggcggggtt
     ttggtcaaaa tttccggact gcgattgtac atgctgttaa cggctccgcc cactattaat
     gaaattaaaa attccaattt taaaaaacgc agcaagagaa acatttgtat gaaagaatgc
     gtagaaggaa agaaaaatgt cgtcgacatg ctgaacaaca agattaatat gcctccgtgt
     ataaaaaaaa tattgaacga tttgaaagaa aacaatgtac cgcgcggcgg tatgtacagg
     aagaggttta tactaaactg ttacattgca aacgtggttt cgtgtgccaa gtgtgaaaac
     cgatgtttaa tcaaggctct gacgcatttc tacaaccacg actccaagtg tgtgggtgaa
     gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg ccaaaaaatg
     aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa tcctatttgt
     aattattgaa taataaaaca attataaatg ctaaatttgt tttttattaa cgatacaaac
     caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag ttataatcgc
     tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac aatataattt
     tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc tctttttcat
     ttttctcctc ataaaaatta acatagttat tatcgtatcc atatatgtat ctatcgtata
     gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt atagtaccgc
     tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct tcggagtgtg
     ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt ttgtacaata
     tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt agcagcaccg
     gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt tcacctccct
     tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc attgtaatga
     gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg ctgatatctc
     cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag tctagactag
     tataccgcgg gccctgcagg ccttaaggcg cgcccgggcg gccgcgtacg attgtaaata
     aaatgtaatt tacagtatag tattttaatt aatatacaaa tgatttgata ataattctta
     tttaactata atatattgtg ttgggttgaa ttaaaggtcc cggcatcctc aaatgcataa
     tttcatagtc ccccttgttg taagtgatgc gtatttctga atctttgtaa aatagcacac
     aagactccaa cgcgtttggc gttttatttt cttgctcgag gatatcatgg agataattaa
     aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata
     aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg tgctagcgga
     tccgagctcg agatctgcag ctggtaccat ggaattcgaa gcttgtcgtt ggatggaaag
     gaaaagagtt ctacagggaa acttggaccc gcttcatgga agacagcttc cccattgtta
     acgaccaaga agtgatggat gttttccttg ttgtcaacat gcgtcccact agacccaacc
     gttgttacaa attcctggcc caacacgctc tgcgttgcga ccccgactat gtacctcatg
     acgtgattag gatcgtcgag ccttcatggg tgggcagcaa caacgagtac cgcatcagcc
     tggctaagaa gggcggcggc tgcccaataa tgaaccttca ctctgagtac accaactcgt
     tcgaacagtt catcgatcgt gtcatctggg agaacttcta caagcccatc gtttacatcg
     gtaccgactc tgctgaagag gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa
     aggagtttgc accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt
     gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt
     gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa
     tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt
     aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat
     ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt
     tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat
     gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt
     taaataaagc tagcttggac atatttaaca tcgggcgtgt tagctttatt aggccgatta
     tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt tgccatagcc
     acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt agttgagctt
     tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac tgtgcccgat
     tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat ttcagacggc
     aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg tggaggcgca
     ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga cggcggttta
     ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact ggtttcgggc
     gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct aatagcttcc
     aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg cattggtgga
     gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg aatgttaggc
     acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt agtttgttcg
     cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg tcgtctgctt
     cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa atcgtaaaaa
     tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa caaccgctca
     atgtaagcaa ttgtattgta aagagattgt ctcaagctcc gcacgccgat aacaagcctt
     ttcattttta ctacagcatt gtagtggcga gacacttcgc tgtcgtcgac tcgagttcta
     tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     tctaacgaca atatgtccat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     tcaccgtcat caccgaaacg cgcgagagga aagggcctcg tgatacgcct atttttatag
     gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     aaaccgcctc tccccgcgcg ttggccgatt cattaatgca ggttaacctg gcttatcgaa
     attaatacga ctcactatag ggagaccggc agatcgatct gtcga
//