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ID   P83        preliminary; circular DNA; SYN; 5376 BP.
XX
AC   A00783;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid p8/3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5376
RC   p8/3 from pBR322
RA   Certa U.;
RT   "Antimalaria vaccines";
RL   Unpublished (1986).
RL   European Patent No. 0309746-A 12 dated 05-APR-1989.
RL   F. HOFFMANN-LA ROCHE AG.
XX
CC   NCBI gi: 14617
CC   NM (p8/3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> p8/3 5376bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5376 BP; 1592 A; 1152 C; 1213 G; 1419 T; 0 other;
     ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
     attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
     ggatcgcatc accatcacca tcacggatcc gagcttgcat gccaatatat gaatgcccca
     aaaaaattac cagcagatgt tgccgaagaa ttagcaacca ccgcccaaaa gcttgttcaa
     gctggaaagg gaattttagc tgctgatgaa tcaacacaaa ccattaagaa aagattcgac
     aacatcaaat tagagaacac aatagaaaac agagctagct acagagattt attatttgga
     actaaaggat taggaaaatt catttcagga gcaattttat ttgaagaaac attatttcaa
     aagaatgaag ccggtgtacc acaggttaat ttattacaca atgaaaatat aattccaggt
     attaaggttg ataaaggttt ggttaacatt ccatgcacag atgaagaaaa atcaactcaa
     tgtttagatg gattagcaga aagatgcaaa gagtattata aagctggtgc aaggtttgct
     aaatggagaa cagttttagt tattgacaca gccaaaggaa aaccaactga tttatcaaat
     cacgaaactg catggggatt ggctagatat gcatctattt gtcaacaaaa tagattagtt
     ccaattgttg aacctgaaat tttagctgat ggaccacact caattgaagt ttgcgcagtt
     gtaactcaaa aagttttatc atgtgtattt aaagctttac aagaaaatgg tgtattatta
     gaaggtgcat tgttaaaacc aaacatggtt actgctggtt atgaatgtac tgctaaaacc
     actactcaag atgttggttt cttaactgtc agaaccttaa ggagaactgt accaccagcc
     ttaccaggtg ttgtattttt atctggagga caatcagaag aagaggcttc tgttaattta
     aattcaatca atgctttggg tccacaccca tgggctttaa ccttctctta cggtagagct
     ttacaagctt cagtattgaa cacatggcaa ggaaagaaag aaaatgttgc aaaggcaaga
     gaagttttat tacaaagagc tgaagccaac tccttagcaa cttatggtaa atacaaagga
     ggtgcaggtg gtgaaaatgc aggtgcttca ttatatgaaa agaaatatgt ctattaaaaa
     cttcaccaac caaaaatgaa taataataat aataaataaa ttactaaatg aatggtacta
     tatttttaaa aataagggta atatattttc tgtatgtata tatatatata tatatacaaa
     atatgtgaaa ttataaaaaa aaaaaaaaaa aaaaaaagga attccggatc ctccggcatc
     atggttcgac cattgaactg catcgtcgcc gtgtcccaaa atatggggat tggcaagaac
     ggagacctac cctggcctcc gctcaggaac gagttcaagt acttccaaag aatgaccaca
     acctcttcag tggaaggtaa acagaatctg gtgattatgg gtaggaaaac ctggttctcc
     attcctgaga agaatcgacc tttaaaggac agaattaata tagttctcag tagagaactc
     aaagaaccac cacgaggagc tcattttctt gccaaaagtt tggatgatgc cttaagactt
     attgaacaac cggaattggc aagtaaagta gacatggttt ggatagtcgg aggcagttct
     gtttaccagg aagccatgaa tcaaccaggc caccttagac tctttgtgac aaggatcatg
     caggaatttg aaagtgacac gtttttccca gaaattgatt tggggaaata taaacttctc
     ccagaatacc caggcgtcct ctctgaggtc caggaggaaa aaggcatcaa gtataagttt
     gaagtctacg agaagaaagg ttccagatct gttaacctag tttaacagga agatgctttc
     aagttctctg ctcccctcct aaagctatgc atttttataa gaccatggga cttttgctgg
     ctttagatcc ggccaagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
     gctagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
     gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa
     ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg
     tgcccttaaa cgcctggggt aatgactctc tagcttgagg catcaaataa aacgaaaggc
     tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     taggacaaat ccgccgctct agagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtctg tcggctgcgg
     cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agctgcctga
     ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     aaaaataggc gtatcacgag gccctttcgt cttcac
//