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ID   PA10       preliminary; circular DNA; SYN; 3980 BP.
XX
AC   IG9808;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E.coli plasmid vector pA10 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pA10 from pBR322
RC   pA10CAT2 from pA10 & linker & pSV2-cat
RC   pSrM11 or pSM11 from pA10CAT2 & linker & pm1sp, Mo-MSV LTR
RC   pSrM12 or pSM12 from pA10CAT2 & linker & pm1sp, Mo-MSV LTR
RC   pL3 from pML2 & pm1sp, Mo-MSV LTR
RC   pSRm15, pSRm16 from pL3 & linker & pA10CAT2
RC   pSrM13, pSrM14 from pL3 & linker & pA10CAT2
RC   pSrM36, pSrM37 from pA10CAT2 & linker & pm1sp, Mo-MSV LTR
RC   pSrM33 from pA10CAT2 & pL3
RC   pML2-poly from pML2 & linker
RC   pA10CAT3M or pCAT3M from pSV2-cat & pML2-poly
RC   pMS-LTR2 from pCAT3M & linker & pm1sp, Mo-MSV LTR
RC   pL1 from pL2
RC   pMS-LTR1 from pCAT3M & pL1
RC   pMS-LTR0 from pCAT3M & pm1sp, Mo-MSV LTR
RC   pMS-LTR15 from pMS-LTR0 & linker & pL2
RC   pMS-LTR10 from pCAT3M & pL2
RC   pMS-LTR16, pMS-LTR17 from pCAT3M & linker & pL2
RC   pMS-LTR38, pMS-LTR39 from pA10CAT2 & linker & pm1sp, Mo-MSV LTR
RC   pMS-SV from SV40 cs1097 & linker & pMS-LTR0
RA   Laimins L.A., Gruss P., Pozzetti R., Khoury G.;
RT   "Characterization of enhancer elements in the long terminal repeat
RT   of Moloney murine sarcoma virus";
RL   J. Virol. 49:183-189(1984).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pA10)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-BamHI 377bp
FT                   \ 4360..4361..376, 3984bp
FT                   blunt end:blunt end
FT                   -> pA10 3984bp [BamHI]"
FT   -               1..3980
FT                   /note="pBR322 380..4359 3980bp
FT                   EcoRI =      G^AATTC
FT                   BamHI = G^GATC C"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
XX
SQ   Sequence 3980 BP; 908 A; 1097 C; 1043 G; 932 T; 0 other;
     ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac
     ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     tcgctcgcgg ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt
     tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca
     tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca
     tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc
     ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc
     tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt
     accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     gaggcccttt cgtcttcaag
//