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ID   PA10CAT    preliminary; circular DNA; SYN; 6915 BP.
XX
AC   IG7004;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pA10CAT - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pA10 from pBR322
RC   pA10CAT2 from pA10 & linker & pSV2-cat
RC   pSrM11 or pSM11 from pA10CAT2 & linker & pm1sp, Mo-MSV LTR
RC   pSrM12 or pSM12 from pA10CAT2 & linker & pm1sp, Mo-MSV LTR
RC   pL3 from pML2 & pm1sp, Mo-MSV LTR
RC   pSRm15, pSRm16 from pL3 & linker & pA10CAT2
RC   pSrM13, pSrM14 from pL3 & linker & pA10CAT2
RC   pSrM36, pSrM37 from pA10CAT2 & linker & pm1sp, Mo-MSV LTR
RC   pSrM33 from pA10CAT2 & pL3
RC   pML2-poly from pML2 & linker
RC   pA10CAT3M or pCAT3M from pSV2-cat & pML2-poly
RC   pMS-LTR2 from pCAT3M & linker & pm1sp, Mo-MSV LTR
RC   pL1 from pL2
RC   pMS-LTR1 from pCAT3M & pL1
RC   pMS-LTR0 from pCAT3M & pm1sp, Mo-MSV LTR
RC   pMS-LTR15 from pMS-LTR0 & linker & pL2
RC   pMS-LTR10 from pCAT3M & pL2
RC   pMS-LTR16, pMS-LTR17 from pCAT3M & linker & pL2
RC   pMS-LTR38, pMS-LTR39 from pA10CAT2 & linker & pm1sp, Mo-MSV LTR
RC   pMS-SV from SV40 cs1097 & linker & pMS-LTR0
RA   Laimins L.A., Gruss P., Pozzetti R., Khoury G.;
RT   "Characterization of enhancer elements in the long terminal repeat
RT   of Moloney murine sarcoma virus";
RL   J. Virol. 49:183-189(1984).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pA10CAT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pSV2-cat)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-BamHI 377bp
FT                   \ 4360..4361..376, 3984bp
FT                   blunt end:blunt end
FT                   -> pA10 3984bp [BamHI]
FT                   1. pA10 remove BamHI-SphI 191bp 0..191,
FT                   \ 3793bp pBR322 376..567
FT                   :BglII linker 6bp agatct
FT                   2. pSV2-cat SphI-BamHI 3112bp 258..3370,
FT                   \ SV40 enh/repeat/RBS/CAT/polyA/pSV2cat 5003bp
FT                   BglII linker 6bp agatct:
FT                   -> pA10CAT2 6917bp"
FT   -               1..3793
FT                   /note="pBR322 567..4359 3793bp
FT                   BamHI = G^GATCC
FT                   BglII = A^GATCT"
FT   -               3794..3798
FT                   /note="gatct 5bp
FT                   BglII = A^GATCT
FT                   SphI =    GCATG^C"
FT   -               3799..6910
FT                   /note="pSV2-cat 258..3369 3112bp
FT                   BamHI = G^GATCC
FT                   BglII = A^GATCT"
FT   -               6911..6915
FT                   /note="gatct 5bp
FT                   BglII = A^GATCT
FT                   SphI =    GCATG^C"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
XX
SQ   Sequence 6915 BP; 1697 A; 1784 C; 1743 G; 1691 T; 0 other;
     caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca
     gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg
     cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
     gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
     aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
     cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
     gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
     atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
     aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
     gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
     acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg
     ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
     atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
     cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat
     ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
     ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
     ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     tttcgtcttc aaggatctct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     caccctaact gacacacatt ccacagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcctttgc ctaatttaaa
     tgaggactta acctgtggaa atattttgat gtgggaagct gttactgtta aaactgaggt
     tattggggta actgctatgt taaacttgca ttcagggaca caaaaaactc atgaaaatgg
     tgctggaaaa cccattcaag ggtcaaattt tcattttttt gctgttggtg gggaaccttt
     ggagctgcag ggtgtgttag caaactacag gaccaaatat cctgctcaaa ctgtaacccc
     aaaaaatgct acagttgaca gtcagcagat gaacactgac cacaaggctg ttttggataa
     ggataatgct tatccagtgg agtgctgggt tcctgatcca agtaaaaatg aaaacactag
     atattttgga acctacacag gtggggaaaa tgtgcctcct gttttgcaca ttactaacac
     agcaaccaca gtgcttcttg atgagcaggg tgttgggccc ttgtgcaaag ctgacagctt
     gtatgtttct gctgttgaca tttgtgggct gtttaccaac acttctggaa cacagcagtg
     gaagggactt cccagatatt ttaaaattac ccttagaaag cggtctgtga aaaaccccta
     cccaatttcc tttttgttaa gtgacctaat taacaggagg acacagaggg tggatgggca
     gcctatgatt ggaatgtcct ctcaagtaga ggaggttagg gtttatgagg acacagagga
     gcttcctggg gatct
//