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ID   PAA113M    preliminary; circular DNA; SYN; 9186 BP.
XX
AC   M20189;
XX
DT   25-APR-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector pAA113M - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-9186
RC   pAA113M from pAA113X & pZ152, M13 ori
RA   Ahmed A.;
RT   "A vector for sequencing long (40-kb) DNA fragments";
RL   Gene 75:315-321(1989).
XX
RN   [2]
RC   pAA113M
RA   Ahmed A.;
RT   ;
RL   Submitted (28-SEP-1988) to GenBank on computer-readable media by:
RL   Ahmed A., .
XX
RN   [3]
RC   delta113 from pAA3.7
RC   pAA113X from delta113
RA   Ahmed A.;
RT   "A simple and rapid procedure for sequencing long (40-kb) DNA
RT   fragments";
RL   Gene 61:363-372(1987).
XX
CC   The cos site is used to clone long segments.
CC   The IS1 is used to subdivide the cloned fragment by deletions.
CC   The galKT is used to select such deletions.
CC   The unique restriction sites are used to further shorten the fragment
CC   for supercoil or M13 sequencing.
CC   It contains a normal plasmid origin of replication and also
CC   the M13 ori of replication to obtain double- or single-stranded DNA.
CC   NM (pAA113M)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (sequencing up to 40000 bp)(supercoiling)
CC   SE (color red/white)
CC   PA (M13)(lambda)(E.coli)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAA3.7 remove IS1-L to cos site, galS gene
FT                   -> delta113
FT                   1. delta113 remove BglII-HpaI, cos/galK
FT                   fill in:
FT                   -> pAA113X 8749bp
FT                   1. pAA113X large ScaI-BamHI
FT                   2. pZ152 large ScaI-BamHI, M13 ori
FT                   -> pAA113M 9186bp"
FT   CDS             3787..4977
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase gene (galKT)"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos region"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13 intergenic region"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="IS1 region"
FT   misc_binding    0..0
FT                   /note="MCS"
XX
SQ   Sequence 9186 BP; 2051 A; 2446 C; 2453 G; 2236 T; 0 other;
     caattactgc aatgccctcg taattaagtg aatttacaat atcgtcctgt tcggagggaa
     gaacgcggga tgttcattct tcatcacttt taattgatgt atatgctctc ttttctgacg
     ttagtctccg acggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgct
     caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg
     ttctgcgggt tctgttcttc gttgacatga ggttgccccg tattcagtgt cgctgatttg
     tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt
     cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagatgat
     aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg cggcgacctc
     gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca
     taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa
     gcgaggcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg
     gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac
     tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat
     gactctgccg ccgtcataaa atggtatgcc gaaagggatg ctgaaattga gaacgaaaag
     ctgcgccggg aggttgaaga actgcggcag gccagcgagg cagatcaaca gtcggtacgg
     ctgaccatcg ggtgccagtg cgggagtttc gttcagcact gtcctgctcc ttgtgatggt
     ttacaaacgt aaaaagtctc tttaatacct gtttttgctt catattgttc agcgacacgt
     tgctgtacgg caggcaccag ctcttccggg atcagcgcga cgatacagcc gccaaatccg
     ccgccggtca tgcgtacgcc acctttgtcg ccaatcacag ctttgacgat ttctaccaga
     gtgtcaattt gcggcacggt gatttcgaaa tcatcgcgca tagaggcatg agactccgcc
     atcaactcgc ccatacgttt caggtcgcct tgctccagcg cgctggcagc ttcaacggtg
     cgggcgtttt cagtcagtat atgacgcacg cgttttgcca cgatcgggtc cagttcatgc
     gcaacagcgt tgaactcttc aatggtgaca tcacgcaggg ctggctgctg gaagaaacgc
     gcaccggttt cgcactgttc acgacgggtg ttgtattcgc tgccaaccag ggtacgtttg
     aagttactgt tgatgatgac gacagccaca cctttgggca tggaaactgc tttggtcccc
     agtgagcggc aatcgatcag caaggcatga tctttcttgc cgagcgcgga aattagctga
     tccatgatcc cgcagttaca gcctacaaac tggttttctg cttcctgacc gttaagcgcg
     atttgtgcgc cgtccagcgg cagatgataa agctgctgca atacggttcc gaccgcgact
     tccagtgaag cggaagaact taacccggca ccctgcggca cattgccgtg atcaaccatg
     tccacgccgc cgaagctgtt gttacgcagt tgcagatgtt tcaccacgcc acgaacgtag
     ttagcccatt gatagttttc atgtgcgaca atgggcgcat cgagggaaaa ctcgtcgagc
     tgattttcat aatcggctgc catcacgcga actttacggt catcgcgtgg tgcacaactg
     atcacggttt gataatcaat cgcgcagggc agaacgaaac cgtcgttgta gtcggtgtgt
     tcaccaatca aattcacgcg gccaggcgcc tgaatggtgt gagtggcagg gtagccaaat
     gcgttggcaa acagagattg tgttttttct ttcagactca tttcttacac tccggattcg
     cgaaaatgga tatcgctgac tgcgcgcaaa cgctctgctg cctgttctgc ggtcaggtct
     cgctgggtct ctgccagcat ttcataacca accataaatt tacgtacggt ggcggacggc
     agcagaggcg gataaaagtg cgcgtgcagc tgccagtgtt gattctcttc gccattaaat
     ggcgcgccgt gccagcccat agagtagggg aaggagcact ggaagaggtt gtcataacga
     ctggtcagct ttttcaacgc cagcgccaga tcgctgcgct gggcgtcggt caaatcggtg
     atccgtaaaa cgtgggcttt gggcagcagt agcgtttcga acggccaggc agcccagtaa
     ggcacgacgg ctaaccagtg ttcggtttcg acaacggtac ggctaccgtc tgccagctcg
     cgctgaacat aatccaccag cattggtgat ttctgttcgg caaaatattc tttttgcagg
     cggtcttcgc gctcagcttc gttaggcagg aagctatttg cccaaatctg accgtgcgga
     tgcgggttag agcagcccat cgccgcgcct ttgttttcaa aaacctgcac ccatgggtac
     gttttcccca gttctgcggt ttgctcctgc caggttttga cgatttccgt caatgctgca
     acgctgagct ctggcagcgt tttactgtga tccggtgaaa agcagatcac ccggctggtg
     ccgcgcgcgc tctggcaacg catcagcgga tcgtgacttt ctggcgcatc tggcgtgtca
     gacatcaaag ccgcaaagtc attagtgaaa acgtaagtcc cggtgtaatc ggggttttta
     tcgcctgtca cccgcacatt acctgcgcag aggaagcaat ctggatcgtg cgcaggtaac
     acctgtttgg ctggcgtttc ctgcgccccc tgccagggcg ttagccgggt gcggtgaaac
     cagaatccat tgcccggtga gcggttgtag cggcgatgtg gatgatcaac gggattaaat
     tgcgtcatgg tcgttcctta atcgggatat ccctgtggat ggcgtgactg ccagtgccag
     gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcagt tcagttcacg gtcggctttg
     ctggcgtccg ccagtaggcc ggaaggtcgc cctcgcgagc ggtgcaaaat gataattaac
     cggtttgccg cacgtttgct gaaggcatta accacgtcca gcacgctgtt gcctacgcca
     gcgccgaggt tgtagatgtg tacgcctggc ttgttcgcca gtttttccat cgccacgacg
     tgaccgtccg ccagatccat tacgtggatg taatcgcgta cgccagtacc atcttcggtc
     ggataatcgt taccaaaaat cgccagcgag tcgcgacggc ctacagcaac ctgggcgatg
     tatggcatca ggttattcgg aatgccttgc ggatcttcgc ccatatcgcc cgacggatgc
     gcgccaaccg ggttgaagta gcgcacgagg gcaatgctcc agtccggctg ggctttttgc
     agatcggtga ggatctgttc caccatcagc ttgcttttgc cgtaagggct ttgcggtgtg
     ccggtcggga agctataatg cggtagttta tcacagttaa attgctaacg cagtcaggca
     ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
     gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc
     gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
     tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
     tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc
     ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac
     ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
     tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca
     tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca
     tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
     ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt gtaaacgtta atattttgtt
     aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg ttccagtttg
     gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg ggtcgaggtg
     ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     acagggcgcg taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcgg taatgactcc aacttattga tagtgtttta tgttcagata
     atgcccgatg actttgtcat gcagctccac cgattttgag aacgacagcg acttccgtcc
     cagccgtgcc aggtgctgcc tcagattcag gttatgccgc tcaattcgct gcgtatatcg
     cttgctgatt acgtgcagct ttcccttcag gcgggattca tacagcggcc agccatccgt
     catccatatc accacgtcaa agggtgacag caggctcata agacgcccca gcgtcgccat
     agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat acgcgtaaaa
     cagccagcgc tggcgcgatt tagccccgac atagccccac tgttcgtcca tttccgcgca
     gacgatgacg tcactgcccg gctgtatgcg cgaggttacc gactgcggcc tgagtttttt
     aagtgacgta aaatcgtgtt gaggccaacg cccataatgc gggctgttgc ccggcatcca
     acgccattca tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa
     gtgaactgca gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg
     gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
     acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
     atcacc
//