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ID   PAA37X     preliminary; circular DNA; SYN; 9583 BP.
XX
AC   L08817; VB0130;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pAA3.7X - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-9583
RC   pAA3.7X
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [2]
RP   2578-2808; 2938-3049; 7876-7889
RC   pAA3.7X from pAA3.7
RA   Ahmed A.;
RT   "A rapid procedure for DNA sequencing using transposon-promoted
RT   deletions in Escherichia coli";
RL   Gene 39:305-310(1985).
XX
RN   [3]
RP   30-2870
RC   pAA3.7X from pAA3.7
RA   Ahmed A.;
RT   "Sequencing with transposon-promoted deletions: Use of
RT   transposon-promoted deletions in DNA sequence analysis";
RL   Methods in Enzymology Recombinant DNA E:0-0(1993).
RL   Wu R., Grossman L., eds., Academic Press, New York.
XX
RN   [4]
RP   1-9583
RC   pAA3.7X
RA   Ahmed A.;
RT   ;
RL   Submitted (00-XXX-1993) to GenBank in computer-readable form by:
RL   Ahmed A., .
XX
RN   [5]
RC   lambda cam1 from lambda & Tn9
RC   pAA3Bcam from pAA3B101 & lambda cam1, Tn9
RC   pAA3.7 from pAA3Bcam & lambda cam1, Tn9
RA   Ahmed A.;
RT   "Use of transposon-promoted deletions in DNA sequence analysis";
RL   J. Mol. Biol. 178:941-948(1984).
XX
RN   [6]
RC   pAA3B101 from pBR322 & lambda gal+ & TRP1 gene
RA   Ahmed A.;
RT   "A deletion analysis of transposon Tn9";
RL   J. Mol. Biol. 173:523-529(1984).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Other pAA-vectors being precursors to pAA3.7X are in Pouwels A-I-i-1.
CC   [1] describes a new quick sequencing method, which involves the
CC   subdivision of the DNA fragment to be sequenced into many overlapping
CC   segments by a series of deletions which start from a fixed site.
CC   Since the different segments retain their natural connectedness, the
CC   complete sequence is easily assembled from overlaps that occur
CC   between the sequences of adjacent deletions. A simple and rapid
CC   method for the isolation of transposon-promoted deletions and their
CC   use in sequencing long DNA fragments was developed.
CC   NM (pAA3.7X)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (PLMCG)(pBR322)(pBR325)(EcoGalK)(M11151)(NNLRSIS)(INS1ECLAC)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII-EcoRI 4330bp 30..4360,
FT                   \ amp/tet genes
FT                   2. lambda gal+ HindIII-EcoRI 8940bp, gal/lambda cos
FT                   3. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS1/TRP1 gene
FT                   -> pAA3B101 14720bp
FT                   1. lambda 48502bp
FT                   transposition
FT                   2. Tn9 5818bp
FT                   -> lambda cam1
FT                   1. pAA3B101 BamHI 14720bp
FT                   2. lambda cam1 BamHI-BamHI 8300bp, Tn9
FT                   -> pAA3Bcam 23000bp
FT                   1. pAA3Bcam 8bp, pBR322 4271..4279
FT                   transposition of Tn9 between amp and TRP1
FT                   2. lambda cam1 17300bp, Tn9
FT                   -> pAA3.7 17300bp
FT                   1. pAA3.7 remove BglII-HpaI, lambda cos
FT                   remove PstI-PstI 360bp, Tn9 right end 1426..1786
FT                   -> pAA3.7X 9583bp"
FT   CDS             complement(30..1359)
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   CDS             1758..1905
FT                   /note="GEN E. coli galactokinase gene (galT) #M11151"
FT   misc_feature    2865..7121
FT                   /note="from pBR322"
FT   CDS             2927..4117
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             complement(6139..6927)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    complement(7122..7889)
FT                   /note="Insertion element Is1K #NNLRSIS
FT                   Insertion element Is1K #INS1ECLAC"
FT   misc_feature    7905..8991
FT                   /note="from pBR325"
FT   CDS             8113..8772
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (cmr/cml)"
FT   misc_feature    complement(8991..9583)
FT                   /note="Insertion element Is1K #NNLRSIS
FT                   Insertion element Is1K #INS1ECLAC"
XX
SQ   Sequence 9583 BP; 2152 A; 2579 C; 2522 G; 2330 T; 0 other;
     ggccttttga aaagcaagca taaaagatca acagtcggta cggctgacca tcgggtgcca
     gtgcgggagt ttcgttcagc actgtcctgc tccttgtgat ggtttacaaa cgtaaaaagt
     ctctttaata cctgtttttg cttcatattg ttcagcgaca cgttgctgta cggcaggcac
     cagctcttcc gggatcagcg cgacgataca gccgccaaat ccgccgccgg tcatgcgtac
     gccacctttg tcgccaatca cagctttgac gatttctacc agagtgtcaa tttgcggcac
     ggtgatttcg aaatcatcgc gcatagaggc atgagactcc gccatcaact cgcccatacg
     tttcaggtcg ccttgctcca gcgcgctggc agcttcaacg gtgcgggcgt tttcagtcag
     tatatgacgc acgcgttttg ccacgatcgg gtccagttca tgcgcaacag cgttgaactc
     ttcaatggtg acatcacgca gggctggctg ctggaagaaa cgcgcaccgg tttcgcactg
     ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt ttgaagttac tgttgatgat
     gacgacagcc acacctttgg gcatggaaac tgctttggtc cccagtgagc ggcaatcgat
     cagcaaggca tgatctttct tgccgagcgc ggaaattagc tgatccatga tcccgcagtt
     acagcctaca aactggtttt ctgcttcctg accgttaagc gcgatttgtg cgccgtccag
     cggcagatga taaagctgct gcaatacggt tccgaccgcg acttccagtg aagcggaaga
     acttaacccg gcaccctgcg gcacattgcc gctgatcacc atgtccacgc cgccgaagct
     gttgttacgc agttgcagat gtttcaccac gccacgaacg tagttagccc attgatagtt
     ttcatgtgcg acaatgggcg catcgaggga aaactcgtcg agctgatttt cataatcggc
     tgccatcacg cgaactttac ggtcatcgcg tggtgcacaa ctgatcacgg tttgataatc
     aatcgcgcag ggcagaacga aaccgtcgtt gtagtcggtg tgttcaccaa tcaaattcac
     gcggccaggc gcctgaatgg tgtgagtggc agggtagcca aatgcgttgg caaacagaga
     ttgtgttttt tctttcagac tcatttctta cactccggat tcgcgaaaat ggatatcgct
     gactgcgcgc aaacgctctg ctgcctgttc tgcggtcagg tctcgctggg tctctgccag
     catttcataa ccaaccataa atttacgtac ggtggcggac gcagcagagg cgataaaagt
     gcgcgtgcag ctgccagtgt tgattctctt cgccattaaa tgggcgccgt gccagcccat
     agagtagggg aaggagcact ggaagaggtt gtcataacga ctggtcagct ttttcaacgc
     cagcgccaga tcgctgcgct gggcgtcggt caaatcggtg atccgtaaaa cgtgggcttt
     gggcagcagt agcgtttcga acggccaggc agcccagtaa ggcacgacgg ctaaccagtg
     ttcggtttcg acaacggtac ggctaccgtc tgccagctcg cgctgaacat aatccaccag
     cattggtgat ttctgttcgg caaaatattc tttttgcagg cggtcttcgc gctcagcttc
     gttaggcagg aagctatttg cccaaatctg accgtgcgga tgcgggttag agcagcccat
     cgccgcgcct ttgttttcaa aaacctgcac ccatgggtac gttttcccca gttctgcggt
     ttgctcctgc caggttttga cgatttccgt caatgctgca acgctgagct ctggcagcgt
     tttactgtga tccggtgaaa agcagatcac ccggctggtg ccgcgcgcgc tctggcaacg
     catcagcgga tcgtgacttt ctggcgcatc tggcgtgtca gacatcaaag ccgcaaagtc
     attagtgaaa acgtaagtcc cggtgtaatc ggggttttta tcgcctgtca cccgcacatt
     acctgcgcag aggaagcaat ctggatcgtg cgcaggtaac acctgtttgg ctggcgtttc
     ctgcgccccc tgccagggcg ttagccgggt gcggtgaaac cagaatccat tgcccggtga
     gcggttgtag cggcgatgtg gatgatcaac gggattaaat tgcgtcatgg tcgttcctta
     atcgggatat ccctgtggat ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc
     gagtgtgcgc gttacgcagt tcagttcacg gtcggctttg ctggcgtccg ccagtaggcc
     ggaaggtcgc cctcgcgagc ggtgcaaaat gataattaac cggtttgccg cacgtttgct
     gaaggcatta accacgtcca gcacgctgtt gcctacgcca gcgccgaggt tgtagatgtg
     tacgcctggc ttgttcgcca gtttttccat cgccacgacg tgaccgtccg ccagatccat
     tacgtggatg taatcgcgta cgccagtacc atcttcggtc ggataatcgt taccaaaaat
     cgccagcgag tcgcgacggc ctacagcaac ctgggcgatg tatggcatca ggttattcgg
     aatgccttgc ggatcttcgc ccatatcgcc cgacggatgc gcgccaaccg ggttgaagta
     gcgcacgagg gcaatgctcc agtccggctg ggctttttgc agatcggtga ggatctgttc
     caccatcagc ttgcttttgc cgtaagggct ttgcggtgtg ccggtcggga agctataatg
     cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa
     tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
     gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta
     tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc
     actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat
     cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt
     ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
     caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
     cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
     aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
     gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
     gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
     atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
     ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaattccccc
     ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     taaacaaata ggggttccgc gcacatttcc ccgaaaagtg cggtaatgac tccaacttat
     tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc caccgatttt
     gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt caggttatgc
     cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt caggcgggat
     tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga cagcaggctc
     ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac aaccgtcttc
     cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc gacatagccc
     cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat gcgcgaggtt
     accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca acgcccataa
     tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt ttctggtgcg
     taccgggttg agaagcggtg taagtgaact gcagttgcca tgttttacgg cagtgagagc
     agagatagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg
     aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata
     cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
     gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg acccggtcgt
     cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta ccggtttatt
     gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt tggtaatgac
     tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc
     caccgatttt gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt
     caggttatgc cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt
     caggcgggat tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga
     cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac
     aaccgtcttc cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc
     gacatagccc cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat
     gcgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca
     acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt
     ttctggtgcg taccgggttg agaagcggtg taagtgaact gca
//