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ID PAA37X preliminary; circular DNA; SYN; 9583 BP.
XX
AC L08817; VB0130;
XX
DT 04-JUN-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pAA3.7X - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-9583
RC pAA3.7X
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
XX
RN [2]
RP 2578-2808; 2938-3049; 7876-7889
RC pAA3.7X from pAA3.7
RA Ahmed A.;
RT "A rapid procedure for DNA sequencing using transposon-promoted
RT deletions in Escherichia coli";
RL Gene 39:305-310(1985).
XX
RN [3]
RP 30-2870
RC pAA3.7X from pAA3.7
RA Ahmed A.;
RT "Sequencing with transposon-promoted deletions: Use of
RT transposon-promoted deletions in DNA sequence analysis";
RL Methods in Enzymology Recombinant DNA E:0-0(1993).
RL Wu R., Grossman L., eds., Academic Press, New York.
XX
RN [4]
RP 1-9583
RC pAA3.7X
RA Ahmed A.;
RT ;
RL Submitted (00-XXX-1993) to GenBank in computer-readable form by:
RL Ahmed A., .
XX
RN [5]
RC lambda cam1 from lambda & Tn9
RC pAA3Bcam from pAA3B101 & lambda cam1, Tn9
RC pAA3.7 from pAA3Bcam & lambda cam1, Tn9
RA Ahmed A.;
RT "Use of transposon-promoted deletions in DNA sequence analysis";
RL J. Mol. Biol. 178:941-948(1984).
XX
RN [6]
RC pAA3B101 from pBR322 & lambda gal+ & TRP1 gene
RA Ahmed A.;
RT "A deletion analysis of transposon Tn9";
RL J. Mol. Biol. 173:523-529(1984).
XX
CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Other pAA-vectors being precursors to pAA3.7X are in Pouwels A-I-i-1.
CC [1] describes a new quick sequencing method, which involves the
CC subdivision of the DNA fragment to be sequenced into many overlapping
CC segments by a series of deletions which start from a fixed site.
CC Since the different segments retain their natural connectedness, the
CC complete sequence is easily assembled from overlaps that occur
CC between the sequences of adjacent deletions. A simple and rapid
CC method for the isolation of transposon-promoted deletions and their
CC use in sequencing long DNA fragments was developed.
CC NM (pAA3.7X)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (PLMCG)(pBR322)(pBR325)(EcoGalK)(M11151)(NNLRSIS)(INS1ECLAC)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 HindIII-EcoRI 4330bp 30..4360,
FT \ amp/tet genes
FT 2. lambda gal+ HindIII-EcoRI 8940bp, gal/lambda cos
FT 3. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS1/TRP1 gene
FT -> pAA3B101 14720bp
FT 1. lambda 48502bp
FT transposition
FT 2. Tn9 5818bp
FT -> lambda cam1
FT 1. pAA3B101 BamHI 14720bp
FT 2. lambda cam1 BamHI-BamHI 8300bp, Tn9
FT -> pAA3Bcam 23000bp
FT 1. pAA3Bcam 8bp, pBR322 4271..4279
FT transposition of Tn9 between amp and TRP1
FT 2. lambda cam1 17300bp, Tn9
FT -> pAA3.7 17300bp
FT 1. pAA3.7 remove BglII-HpaI, lambda cos
FT remove PstI-PstI 360bp, Tn9 right end 1426..1786
FT -> pAA3.7X 9583bp"
FT CDS complement(30..1359)
FT /note="GEN E. coli galactokinase gene (galK)"
FT CDS 1758..1905
FT /note="GEN E. coli galactokinase gene (galT) #M11151"
FT misc_feature 2865..7121
FT /note="from pBR322"
FT CDS 2927..4117
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT CDS complement(6139..6927)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_feature complement(7122..7889)
FT /note="Insertion element Is1K #NNLRSIS
FT Insertion element Is1K #INS1ECLAC"
FT misc_feature 7905..8991
FT /note="from pBR325"
FT CDS 8113..8772
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (cmr/cml)"
FT misc_feature complement(8991..9583)
FT /note="Insertion element Is1K #NNLRSIS
FT Insertion element Is1K #INS1ECLAC"
XX
SQ Sequence 9583 BP; 2152 A; 2579 C; 2522 G; 2330 T; 0 other;
ggccttttga aaagcaagca taaaagatca acagtcggta cggctgacca tcgggtgcca
gtgcgggagt ttcgttcagc actgtcctgc tccttgtgat ggtttacaaa cgtaaaaagt
ctctttaata cctgtttttg cttcatattg ttcagcgaca cgttgctgta cggcaggcac
cagctcttcc gggatcagcg cgacgataca gccgccaaat ccgccgccgg tcatgcgtac
gccacctttg tcgccaatca cagctttgac gatttctacc agagtgtcaa tttgcggcac
ggtgatttcg aaatcatcgc gcatagaggc atgagactcc gccatcaact cgcccatacg
tttcaggtcg ccttgctcca gcgcgctggc agcttcaacg gtgcgggcgt tttcagtcag
tatatgacgc acgcgttttg ccacgatcgg gtccagttca tgcgcaacag cgttgaactc
ttcaatggtg acatcacgca gggctggctg ctggaagaaa cgcgcaccgg tttcgcactg
ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt ttgaagttac tgttgatgat
gacgacagcc acacctttgg gcatggaaac tgctttggtc cccagtgagc ggcaatcgat
cagcaaggca tgatctttct tgccgagcgc ggaaattagc tgatccatga tcccgcagtt
acagcctaca aactggtttt ctgcttcctg accgttaagc gcgatttgtg cgccgtccag
cggcagatga taaagctgct gcaatacggt tccgaccgcg acttccagtg aagcggaaga
acttaacccg gcaccctgcg gcacattgcc gctgatcacc atgtccacgc cgccgaagct
gttgttacgc agttgcagat gtttcaccac gccacgaacg tagttagccc attgatagtt
ttcatgtgcg acaatgggcg catcgaggga aaactcgtcg agctgatttt cataatcggc
tgccatcacg cgaactttac ggtcatcgcg tggtgcacaa ctgatcacgg tttgataatc
aatcgcgcag ggcagaacga aaccgtcgtt gtagtcggtg tgttcaccaa tcaaattcac
gcggccaggc gcctgaatgg tgtgagtggc agggtagcca aatgcgttgg caaacagaga
ttgtgttttt tctttcagac tcatttctta cactccggat tcgcgaaaat ggatatcgct
gactgcgcgc aaacgctctg ctgcctgttc tgcggtcagg tctcgctggg tctctgccag
catttcataa ccaaccataa atttacgtac ggtggcggac gcagcagagg cgataaaagt
gcgcgtgcag ctgccagtgt tgattctctt cgccattaaa tgggcgccgt gccagcccat
agagtagggg aaggagcact ggaagaggtt gtcataacga ctggtcagct ttttcaacgc
cagcgccaga tcgctgcgct gggcgtcggt caaatcggtg atccgtaaaa cgtgggcttt
gggcagcagt agcgtttcga acggccaggc agcccagtaa ggcacgacgg ctaaccagtg
ttcggtttcg acaacggtac ggctaccgtc tgccagctcg cgctgaacat aatccaccag
cattggtgat ttctgttcgg caaaatattc tttttgcagg cggtcttcgc gctcagcttc
gttaggcagg aagctatttg cccaaatctg accgtgcgga tgcgggttag agcagcccat
cgccgcgcct ttgttttcaa aaacctgcac ccatgggtac gttttcccca gttctgcggt
ttgctcctgc caggttttga cgatttccgt caatgctgca acgctgagct ctggcagcgt
tttactgtga tccggtgaaa agcagatcac ccggctggtg ccgcgcgcgc tctggcaacg
catcagcgga tcgtgacttt ctggcgcatc tggcgtgtca gacatcaaag ccgcaaagtc
attagtgaaa acgtaagtcc cggtgtaatc ggggttttta tcgcctgtca cccgcacatt
acctgcgcag aggaagcaat ctggatcgtg cgcaggtaac acctgtttgg ctggcgtttc
ctgcgccccc tgccagggcg ttagccgggt gcggtgaaac cagaatccat tgcccggtga
gcggttgtag cggcgatgtg gatgatcaac gggattaaat tgcgtcatgg tcgttcctta
atcgggatat ccctgtggat ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc
gagtgtgcgc gttacgcagt tcagttcacg gtcggctttg ctggcgtccg ccagtaggcc
ggaaggtcgc cctcgcgagc ggtgcaaaat gataattaac cggtttgccg cacgtttgct
gaaggcatta accacgtcca gcacgctgtt gcctacgcca gcgccgaggt tgtagatgtg
tacgcctggc ttgttcgcca gtttttccat cgccacgacg tgaccgtccg ccagatccat
tacgtggatg taatcgcgta cgccagtacc atcttcggtc ggataatcgt taccaaaaat
cgccagcgag tcgcgacggc ctacagcaac ctgggcgatg tatggcatca ggttattcgg
aatgccttgc ggatcttcgc ccatatcgcc cgacggatgc gcgccaaccg ggttgaagta
gcgcacgagg gcaatgctcc agtccggctg ggctttttgc agatcggtga ggatctgttc
caccatcagc ttgcttttgc cgtaagggct ttgcggtgtg ccggtcggga agctataatg
cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa
tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta
tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc
actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat
cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt
ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaattccccc
ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
taaacaaata ggggttccgc gcacatttcc ccgaaaagtg cggtaatgac tccaacttat
tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc caccgatttt
gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt caggttatgc
cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt caggcgggat
tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga cagcaggctc
ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac aaccgtcttc
cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc gacatagccc
cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat gcgcgaggtt
accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca acgcccataa
tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt ttctggtgcg
taccgggttg agaagcggtg taagtgaact gcagttgcca tgttttacgg cagtgagagc
agagatagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg
aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata
cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg acccggtcgt
cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta ccggtttatt
gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt tggtaatgac
tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc
caccgatttt gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt
caggttatgc cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt
caggcgggat tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga
cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac
aaccgtcttc cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc
gacatagccc cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat
gcgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca
acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt
ttctggtgcg taccgggttg agaagcggtg taagtgaact gca
//