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ID   PAC360     preliminary; circular DNA; SYN; 9828 BP.
XX
AC   IG1022; A13228; A13229; A13230;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pAc360 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pAc101, pAc311, pAc360 from AcMNPV
RC   pAc373 from AcMNPV
RC   pAc373-CAT from pAc373
RC   pAc401, pAc409, pAc436, pAc461 from pAc311
RC   pAc461-RI-, pAc461-RI/PstI, pAc461-PstI from pAc461
RC   pAc510 from AcMNPV
RC   pAc610, pAc611 from pAc461
RC   pAc700, pAc701, pAc702 from pAc373
RC   pAcRP6, pAcRP18 from AcMNPV
RC   pAcYM1 from AcMNPV
RC   pVL77 from pUC19
RC   pVL101 from pAc401
RC   pVL106 from pAc436
RC   pAcC4, pAcC5 from AcMNPV
RA   Luckow V.A., Summers M.D.;
RT   "Trends in the development of baculovirus expression vectors";
RL   Biotechnology 6:47-55(1988).
XX
RN   [2]
RC   pI8 from pUC8 & AcNPV EcoRI-1
RC   pAc101 from pI8
RC   pB' from pUC8 & AcNPV EcoRI-1
RC   pB'-mutant from pB' & pUC8
RC   pAc311 from pAc101 & pB'-mutant
RC   pAc360 from pAc101 & pB'-mutant
RC   pAc380 from pAc101 & pB'-mutant
RA   Smith G.E., Summers M.D., Fraser M.J.;
RT   "Production of human beta interferon in insect cells infected
RT   with a baculovirus expression vector";
RL   Mol. Cell. Biol. 3:2156-2165(1983).
XX
RN   [3]
RC   pIL-2-2b from interleukin 2 gene
RC   from pAc373 & IL-2 gene
RC   from pAc380 & IL-2 gene
RA   Smith G.E., Ju G., Ericson B.L., Moschera J., Lahm H., Chizzonite R.,
RA   Summers M.D.;
RT   "Modification and secretion of human interleukin 2 produced in
RT   insect cells by a baculovirus expression vector";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:8404-8408(1985).
XX
RN   [4]
RC   39-50, 81-268, 83-174, 86-139 from pBR322 & LCMV WE S
RC   B-13 from 39-50 & 81-268
RC   86-139-1 from pBR322 & 86-139
RC   A-3 from 83-174 & 86-139-1
RC   Y-1 from B-13 & A-3
RC   Y-1-A from pBR322 & Y-1
RC   pAcRP1-LCM WE G, pAcRP1-LCM WE N from pAcRP1 & Y-1-A
RC   pAcRP5-LCM WE G, pAcRP5-LCM WE N from pAcRP5 & Y-1-A
RC   pAcRP6-LCM WE G, pAcRP6-LCM WE N from pAcRP6 & Y-1-A
RC   pAcRP5-LCM WE S from pAcRP5-LCM WE G & pAcRP5-LCM WE N
RC   YN6, YN12, YN13 from pAcRP5-LCM WE S
RC   YG5 from pAcRP5-LCM WE G
RC   A251 from pAcRP1-LCM WE N
RA   Matsuura Y., Possee R.D., Bishop D.H.L.;
RT   "Expression of the S-coded genes of lymphocytic choriomeningitis
RT   arenavirus using a baculovirus vector";
RL   J. Gen. Virol. 67:1515-1529(1986).
XX
RN   [5]
RC   pEVIV from pUC8 & AcMNPV, polyhedrin gene
RC   pEV/55 from pEVIV
RC   pEV/cc from pEV/55
RA   Miller D.W., Safer P., Miller L.K.;
RT   "An insect baculovirus host-vector system for high-level
RT   expression of foreign genes";
RL   Genet. Eng. 8:277-298(1986).
XX
CC   NM (pAc360)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF (pVL1392)(pVL1393)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 EcoRI 2665bp 231..231, MCS
FT                   2. AcNPV EcoRI-EcoRI, polyhedrin gene EcoRI fragment 1
FT                   -> pI8
FT                   1. pI8 PstI-SmaI, in pUC8 part
FT                   S1 nuclease
FT                   BamHI, 3' to polyhedrin gene
FT                   S1 nuclease
FT                   -> pAc101 [unique BamHI in polyhedrin]
FT                   1. pUC8 remove EcoRI-BamHI 10bp 231.241, MCS/2655bp
FT                   2. AcNPV EcoRI-BamHI, EcoRI fragment 1 0..4210
FT                   -> pB'
FT                   1. pB' BamHI
FT                   BAL31 nuclease
FT                   Klenow:Klenow
FT                   BamHI linker 8bp cggatccg:BamHI linker 8bp cggatccg
FT                   BamHI-EcoRI
FT                   2. pUC8 remove EcoRI-BamHI 10bp 231.241, MCS/2655bp
FT                   -> pB'-mutant
FT                   1. pAc101 XhoI-BamHI 2310bp, EcoRI frag 1 1900..4210
FT                   2. pB'-mutant XhoI-BamHI
FT                   -> pAc360 9828bp"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    868..868
FT                   /note="SIT SacII"
FT   misc_binding    1395..1395
FT                   /note="SIT ApaI"
FT   misc_binding    1901..1901
FT                   /note="SIT XhoI"
FT   misc_binding    3998..3998
FT                   /note="SIT EcoRV"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    4128..4128
FT                   /note="SIT BamHI"
FT   misc_binding    4588..4588
FT                   /note="SIT KpnI"
FT   misc_binding    7194..7194
FT                   /note="SIT EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 9828 BP; 2679 A; 2153 C; 2210 G; 2786 T; 0 other;
     aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
     gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
     ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
     gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
     ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
     gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
     gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
     ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
     cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
     aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
     tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
     tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
     gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
     ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
     cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
     ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
     tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     aaacctataa atatgccgga ttattcatac cgtcccacca tcgggccgga tcctttcctg
     ggacccggca agaaccaaaa actcactctc ttcaaggaaa tccgtaatgt taaacccgac
     acgatgaagc ttgtcgttgg atggaaagga aaagagttct acagggaaac ttggacccgc
     ttcatggaag acagcttccc cattgttaac gaccaagaag tgatggatgt tttccttgtt
     gtcaacatgc gtcccactag acccaaccgt tgttacaaat tcctggccca acacgctctg
     cgttgcgacc ccgactatgt acctcatgac gtgattagga tcgtcgagcc ttcatgggtg
     ggcagcaaca acgagtaccg catcagcctg gctaagaagg gcggcggctg cccaataatg
     aaccttcact ctgagtacac caactcgttc gaacagttca tcgatcgtgt catctgggag
     aacttctaca agcccatcgt ttacatcggt accgactctg ctgaagagga ggaaattctc
     cttgaagttt ccctggtgtt caaagtaaag gagtttgcac cagacgcacc tctgttcact
     ggtccggcgt attaaaacac gatacattgt tattagtaca tttattaagc gctagattct
     gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
     tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
     ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
     tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt
     gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat
     aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta
     gtgtacaatt gactcgacgt aaacacgtta aataaagctt ggacatattt aacatcgggc
     gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct
     tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc
     gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg tttttgggcg
     ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag cgatggtgca
     ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg agctgatgat
     aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg cggaggtggt
     ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac agtcggcacc
     tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag acgagtgcga
     tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt
     tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg tggtggtggt
     ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc cgccggtata
     atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc
     tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta
     taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct atcgtttacc
     gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag
     ctcgccgcac gccgataaca agccttttca tttttactac agcattgtag tggcgagaca
     cttcgctgtc gtcgacgtac atgtatgctt tgttgtcaaa aacgtcgttg gcaagcttta
     aaatatttaa aagaacatct ctgttcagca ccactgtgtt gtcgtaaatg ttgtttttga
     taatttgcgc ttccgcagta tcgacacgtt caaaaaattg atgcgcatca attttgttgt
     tcctattatt gaataaataa gattgtacag attcatatct acgattcgtc atggccacca
     caaatgctac gctgcaaacg ctggtacaat tttacgaaaa ctgcaaaaac gtcaaaactc
     ggtataaaat aatcaacggg cgctttggca aaatatctat tttatcgcac aagcccacta
     gcaaattgta tttgcagaaa acaatttcgg cgcacaattt taacgctgac gaaataaaag
     ttcaccagtt aatgagcgac cacccaaatt ttataaaaat ctattttaat cacggttcca
     tcaacaacca agtgatcgtg atggactaca ttgactgtcc cgatttattt gaaacactac
     aaattaaagg cgagctttcg taccaacttg ttagcaatat tattagacag ctgtgtgaag
     cgctcaacga tttgcacaag cacaatttca tacacaacga cataaaactc gaaaatgtct
     tatatttcga agcacttgat cgcgtgtatg tttgcgatta cggattgtgc aaacacgaaa
     actcacttag cgtgcacgac ggcacgttgg agtattttag tccggaaaaa attcgacaca
     caactatgca cgtttcgttt gactggtacg cggcgtgtta acatacaagt tgctaaccgg
     cggccgacac ccatttgaaa aaagcgaaga cgaaatgttg gacttgaata gcatgaagcg
     tcgtcagcaa tacaatgaca ttggcgtttt aaaacacgtt cgtaacgtta acgctcgtga
     ctttgtgtac tgcctaacaa gatacaacat agattgtaga ctcacaaatt acaaacaaat
     tataaaacat gagtttttgt cgtaaaaatg ccacttgttt tacgagtaga attgaattcg
     taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgcc
//