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ID   PACUW31    preliminary; circular DNA; SYN; 8532 BP.
XX
AC   IG1023;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pAcUW31 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pAcRP19 from AcMNPV; [similar to pAcRP18]
RC   pAcRP19.HA from pAcRP19 & A/PR/8/34 HA gene
RC   pAcRP25-BglII from pAcRP25 & linker
RC   pAcRP25-BglII-p10 from pAcRP25-BglII & pAcp10+1
RC   pAcUW3 from pAcRP25-BglII-p10 & pAcUW2
RA   Weyer U., Possee R.D.;
RT   "A baculovirus dual expression vector derived from the Autographa
RT   californica nuclear polyhedrosis virus polyhedrin and p10
RT   promoters: co-expression of two influenza virus genes in insect
RT   cells";
RL   J. Gen. Virol. 72:2967-2974(1991).
XX
RN   [2]
RC   pBR322.BTV-10.7, pBR322.BTV-17.3 from pBR322 & BTV
RC   pUC4.BTV-10.7 from pBR322.BTV-10.7 & pUC4K
RC   pUC4.BTV-17.3 from pBR322.BTV-17.3 & pUC4K
RC   pAcVC3.BTV-10.7 from pUC4.BTV-10.7 & pAcVC3
RC   pAcVC3.BTV-10.7.BTV-17.3 from pUC4.BTV-17.3 & pAcVC3.BTV-10.7
RA   French T.J., Roy P.;
RT   "Synthesis of bluetongue virus (BTV) corelike particles by a
RT   recombinant baculovirus expressing the two major structural core
RT   proteins of BTV";
RL   J. Virol. 64:1530-1536(1990).
XX
RN   [3]
RC   pAcUW31 from pAcUW3
RA   Lopez-Ferber M., Possee R.D.;
RT   ;
RL   Unpublished (1992).
XX
RN   [4]
RC   pAcEcoRI from pUC8 & AcNPV
RC   plasmid from pAcYM1 & pAcEcoRI
RC   pAcVC2 from plasmid & LCMV N protein gene
RC   [pAcVC3 from pAcVC2]
RA   Emery V.C., Bishop D.H.;
RT   "The development of multiple expression vectors for the high level
RT   synthesis of eukaryotic proteins: expression of LCMV-N and AcNPV
RT   polyhedrin protein by a recombinant baculovirus";
RL   Protein Engineering 1:359-366(1987).
XX
CC   NM (pAcUW31)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (CLONTECH)
CC   HO (Spodoptera frugiperda IPLB-SF21)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE ()
CC   PA (pAcUW3)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAcRP25 EcoRV
FT                   BglII linker 6bp agatct
FT                   -> pAcRP25-BglII
FT                   1. pAcRP25-BglII BglII
FT                   2. pAcp10+1 BglII-BamHI 230bp, p10 promoter
FT                   -> pAcRP25-BglII-p10
FT                   1. pAcRP25-BglII-p10 BglII-SnaBI 427bp, p10/polyh prom
FT                   2. pAcUW2 remove BglII-SnaBI 1170bp, polyh
FT                   -> pAcUW3 10040bp [BamHI in polyh prom, BglII in p10]
FT                   1. pAcUW3
FT                   -> pAcUW31 8532bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    235..235
FT                   /note="SIT SphI"
FT   misc_binding    1256..1256
FT                   /note="SIT BstXI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    2552..2552
FT                   /note="SIT EcoRI"
FT   misc_binding    2558..2558
FT                   /note="SIT BglII"
FT   promoter        0..0
FT                   /note="PRO AcMNPV p10 gene"
FT   misc_binding    2797..2797
FT                   /note="SIT XbaI"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    2918..2918
FT                   /note="SIT BamHI"
FT   polyA_signal    0..0
FT                   /note="PLA AcMNPV polyhedrin gene"
FT   misc_binding    3352..3352
FT                   /note="SIT HindIII"
FT   misc_binding    4398..4398
FT                   /note="SIT HindIII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   misc_binding    5889..5889
FT                   /note="SIT DraIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    6804..6804
FT                   /note="SIT ScaI"
FT   misc_binding    7764..7764
FT                   /note="SIT AlwNI"
XX
SQ   Sequence 8532 BP; 2370 A; 1823 C; 1899 G; 2440 T; 0 other;
     tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
     accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
     ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
     ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
     gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
     ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
     tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
     atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
     cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
     ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
     tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
     gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
     ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
     cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
     tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
     ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
     taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc
     agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     ctgcaataaa caagttccga gtttgtcaga aagcagacca aacagcggtt ggaataatag
     cgagaacaga gaaatagcgg caaaaataat acccgtatca cttttgctga tatggttgat
     gtcatgtagc caaatcggga aaaacgggaa gtaggctccc atgataaaaa agtaaaagaa
     aaagaataaa ccgaacatcc aaaagtttgt gttttttaaa tagtacataa tggatttcct
     tacgcgaaat acgggcagac atggcctgcc cggttattat tatttttgac accagaccaa
     ctggtaatgg tagcgaccgg cgctcagctg gaattcagat ctgtgattgt aaataaaatg
     taatttacag tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa
     ctataatata ttgtgttggg ttgaattaaa ggtcccggca tcctcaaatg cataatttca
     tagtccccct tgttgtaagt gatgcgtatt tctgaatctt tgtaaaatag cacacaagac
     tccaacgcgt ttggcgtttt attttcttgc tcgactctag aggatcgatc ccccggatct
     gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt
     cgtaacagtt ttgtaataaa aaaacctata aatatcggat ccgggttatt agtacattta
     ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
     ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
     gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
     agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
     caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
     tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
     ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata aagcttggac
     atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
     gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
     gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaatta tttctgattg
     cgggcgtttt tgggcgggtt tcaatctaac tgtgcccgat tttaattcag acaacacgtt
     agaaagcgat ggtgcaggcg gtggtaacat ttcagacggc aaatctacta atggcggcgg
     tggtggagct gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg
     cggaggcgga ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg
     caacacagtc ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg
     tctgagacga gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc
     taaaggtgca gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat
     cgatggtggt ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg
     cggtgccgcc ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg
     cgcaggcgcc gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg
     tggcaattca atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat
     ttcgctatcg tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta
     aagagattgt ctcaagctcg gatcgatccc gcacgccgat aacaagcctt ttcattttta
     ctacagcatt gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt
     caaaaacgtc gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg
     tgttgtcgta aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa
     attgatgcgc atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat
     atctacgatt cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg
     aaaactgcaa aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat
     ctattttatc gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca
     attttaacgc tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa
     aaatctattt taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact
     gtcccgattt atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca
     atattattag acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca
     acgacataaa actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg
     attacggatt gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt
     ttagtccgga aaaaattcga cacacaacta tgcacgtttc gtttgactgg tacgcggcgt
     gttaacatac aagttgctaa ccggcggccg acacccattt gaaaaaagcg aagacgaaat
     gttggacttg aatagcatga agcgtcgtca gcaatacaat gacattggcg ttttaaaaca
     cgttcgtaac gttaacgctc gtgactttgt gtactgccta acaagataca acatagattg
     tagactcaca aattacaaac aaattataaa acatgagttt ttgtcgtaaa aatgccactt
     gttttacgag tagaattagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     tgattacgaa tt
//