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ID   PACYC184   preliminary; circular DNA; SYN; 4245 BP.
XX
AC   X06403; ATCC37033;
XX
DT   02-APR-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pACYC184 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4244
RC   pACYC184
RA   Rose R.E.;
RT   ;
RL   Submitted (15-DEC-1987) on tape to the EMBL Data Library by:
RL   Rose R.E., Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA.
XX
RN   [2]
RP   1-944, 946-4245
RC   pACYC184 from pACYC175
RA   Rose R.E.;
RT   "The nucleotide sequence of pACYC184";
RL   Nucleic Acids Res. 16:355-355(1988).
XX
RN   [3]
RC   pACYC139 from p15A & pSC105
RC   pACYC140 from p15A & pSC105
RC   pACYC142 from pACYC140 & pSC204, Tn3
RC   pACYC175 from pACYC144
RC   pACYC177 from pACYC175
RC   pACYC160 from p15A & pSC101
RC   pACYC161 from pACYC160 & pSC212
RC   pACYC175 from pACYC161 & pKT002
RC   pACYC184 from pACYC175
RA   Chang A.C., Cohen S.N.;
RT   "Construction and characterization of amplifiable multicopy DNA
RT   cloning vehicles derived from the P15A cryptic miniplasmid";
RL   J. Bacteriol. 134:1141-1156(1978).
XX
RN   [4]
RC   from lambda
RC   from pBR328
RC   from pACYC177
RC   from pACYC184
RA   Li J.K., Tu J.;
RT   "Ssp RFI, a novel class-II restriction endonucleasse from
RT   Synechococcus RF-1 recognizing 5'TT/CGAA-3'";
RL   Nucleic Acids Res. 19:4770-4770(1991).
XX
CC   The sequence is circular; however, the sequence begins with a unique
CC   EcoRI site.
CC   Bases 426-706, 1153-1583, and 2918 to 3735 were determined
CC   while the rest of the sequence was taken from the
CC   published sequence of pSC101, Tn9, and p15A.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--4.3; EcoRI/BamHI--2.4, 1.9. (ATCC staff)
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pACYC184)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (NEB)(ATCC)
CC   HO (E.coli PM191)(E.coli C600 chi1776)(E.coli)
CC   CP (low)
CC   FN (cloning)
CC   SE ()
CC   PA (Tn9)(pSC101)(p15A)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. p15A HincII-HincII 1100bp
FT                   reverse transcriptase fill in:reverse transcriptase
FT                   \ fill in
FT                   2. pSC101 EcoRI 9263bp 9262..9262
FT                   -> pACYC160 10400bp
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   -> pSC105 16200bp
FT                   1. R1
FT                   -> R1-19
FT                   1. R1-19, Tn3/Tn4 amp gene
FT                   -> pSC50
FT                   1. pSC105 16200bp
FT                   2. pSC50, Tn3 amp gene
FT                   -> pSC211
FT                   1. pSC211
FT                   -> pSC212 [no tet]
FT                   1. pACYC160
FT                   transposition
FT                   2. pSC212
FT                   -> pACYC161 3000bp
FT                   1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. pVH51 EcoRI 3847bp 3846..3846
FT                   -> pML21 10747bp
FT                   1. pML21 10747bp
FT                   2. Tn9 HhaI-HhaI 1364bp, cat gene/#V00622
FT                   \ pBR328 3170..4534
FT                   -> pKT002
FT                   1. pACYC161 HindIII 3000bp, tet gene/pSC101 30..30
FT                   2. pKT002 HindIII-PstI 9000bp, cat gene
FT                   -> pACYC175 12000bp
FT                   1. pACYC175 remove small AluI-HaeIII
FT                   -> pACYC184 4245bp"
FT   CDS             complement(3804..4244)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   CDS             complement(1..219))
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_binding    1..1
FT                   /note="SIT unique EcoRI"
FT   misc_feature    443..583
FT                   /note="TRN part of IS1 from Tn9"
FT   rep_origin      581..1492
FT                   /note="ORI E. coli miniplasmid p15A"
FT   misc_feature    complement(3530..4245, 1..585)
FT                   /note="TRN Tn9 HaeII fragment 1300bp"
FT   misc_binding    594..594
FT                   /note="SIT unique Bst1107I"
FT   misc_binding    635..635
FT                   /note="SIT unique XmnI"
FT   misc_binding    832..832
FT                   /note="SIT unique SacII"
FT   conflict        944..946
FT                   /note="replace(944..946,tc); ref. [1] & [2]"
FT   misc_binding    959..959
FT                   /note="SIT unique BsiI"
FT   misc_binding    1405..1405
FT                   /note="SIT unique AseI"
FT   misc_binding    1424..1424
FT                   /note="SIT unique XbaI"
FT   misc_binding    1446..1446
FT                   /note="SIT unique EarI"
FT   misc_feature    1494..3275
FT                   /note="pSC101 fragment"
FT   misc_binding    1517..1517
FT                   /note="SIT unique ClaI"
FT   misc_binding    1523..1523
FT                   /note="SIT unique HindIII"
FT   CDS             1580..2770
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    1632..1632
FT                   /note="SIT unique SfcI"
FT   misc_binding    1679..1679
FT                   /note="SIT unique EcoRV"
FT   misc_binding    1869..1869
FT                   /note="SIT unique BamHI"
FT   misc_binding    1983..1983
FT                   /note="SIT unique BspHI"
FT   misc_binding    2056..2056
FT                   /note="SIT unique SphI"
FT   misc_binding    2116..2116
FT                   /note="SIT unique EcoNI"
FT   misc_binding    2145..2145
FT                   /note="SIT unique SalI"
FT   misc_binding    2433..2433
FT                   /note="SIT unique EagI"
FT   misc_binding    2466..2466
FT                   /note="SIT unique NruI"
FT   misc_binding    2539..2539
FT                   /note="SIT unique ApaBI"
FT   misc_binding    2557..2557
FT                   /note="SIT unique BspMI"
FT   misc_binding    2919..2919
FT                   /note="SIT unique AvaI"
FT   misc_binding    3175..3175
FT                   /note="SIT unique DrdII"
FT   misc_binding    3203..3203
FT                   /note="SIT unique Eam1105I"
FT   misc_binding    3441..3441
FT                   /note="SIT unique BsaBI"
FT   misc_binding    3541..3541
FT                   /note="SIT unique BclI"
FT   misc_binding    3591..3591
FT                   /note="SIT unique Bsu36I"
FT   misc_binding    3695..3695
FT                   /note="SIT unique DrdI"
FT   misc_binding    3698..3698
FT                   /note="SIT unique Tth111I"
FT   misc_binding    3830..3830
FT                   /note="SIT unique ScaI"
FT   misc_binding    3944..3944
FT                   /note="SIT unique NcoI"
FT   misc_binding    4233..4233
FT                   /note="SIT unique BsrDI"
XX
SQ   Sequence 4245 BP; 956 A; 1167 C; 1094 G; 1028 T; 0 other;
     gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
     gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
     ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct
     tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
     tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
     tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
     tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
     tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga
     tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
     gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
     cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
     agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
     catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg
     gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg
     ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg
     caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
     tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc
     aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta
     ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat
     gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
     acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa
     atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
     tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
     acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
     aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg
     gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
     taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat
     cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     aacactatcc catatcacca gctcaccgtc tttcattgcc atacg
//